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Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. PNGase F Mixture 1. protocols.io dx.doi.org/10.17504/protocols.io.cqgvtv Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Protocol for blunting ends by 3' overhang removal and fill-in of 3' recessed (5' overhang) ends using T4 DNA Polymerase.
Proper citation: New England Biolabs 2015. Blunting Protocol (M0203). protocols.io dx.doi.org/10.17504/protocols.io.cgwtxd Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the protocol for Protein Expression Using BL21(DE3) Competent E. coli (C2527). For large scale, please follow this protocol variant.
Proper citation: New England Biolabs 2014. Protein Expression Using BL21(DE3) (C2527). protocols.io dx.doi.org/10.17504/protocols.io.cjqumv Copy
Authors: NEB
Group: New England Biolabs (NEB)
Proper citation: NEB 2016. Digestion Mixture for M0302 (v2). protocols.io dx.doi.org/10.17504/protocols.io.erhbd36 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The BioBrick® Assembly Kit was developed in partnership with Ginkgo BioWorks. What follows is an abbreviated set of protocols for the use of the BioBrick® Assembly Kit (to assemble an Upstream Part with a Downstream Part into a Destination Plasmid). For more details and for technical questions, please see here.
Proper citation: New England Biolabs 2015. Digestion for BioBrick Assembly Kit (E0546). protocols.io dx.doi.org/10.17504/protocols.io.cphvj5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is a protocol for a typical DNase I Reaction, using the M0303 RNase-free DNase I.
Proper citation: New England Biolabs 2015. A Typical DNase I Reaction (M0303). protocols.io dx.doi.org/10.17504/protocols.io.cqevtd Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Digestion Mixture for M0302. protocols.io dx.doi.org/10.17504/protocols.io.cqrvv5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Protocol for Dephosphorylation of 5´-ends of DNA using rSAP (M0371)
Proper citation: New England Biolabs 2015. Dephosphorylation of 5´-ends of DNA using rSAP (M0371). protocols.io dx.doi.org/10.17504/protocols.io.crkv4v Copy
Authors: Isabel Gautreau
Group: New England Biolabs (NEB)
Summary: Protocol for digestion with NEBNext dsDNA Fragmentase (M0348)
Proper citation: Isabel Gautreau 2015. Digestion with NEBNext dsDNA Fragmentase (M0348). protocols.io dx.doi.org/10.17504/protocols.io.cr4v8v Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina contains the enzymes and buffers required to convert a broad range of input amounts of RNA into high quality directional (strand-specific) libraries for next-generation sequencing on the Illumina platform. The fast, user-friendly workflow has minimal hands-on time and is compatible with poly(A) mRNA enrichment and rRNA depletion methods.
Proper citation: New England Biolabs 2018. Protocol for use with rRNA Depleted FFPE RNA and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765). protocols.io dx.doi.org/10.17504/protocols.io.ubaesie Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the PCR protocol for Phusion® High-Fidelity DNA Polymerase (M0530)
Proper citation: New England Biolabs 2015. PCR with Phusion® Polymerase (M0530). protocols.io dx.doi.org/10.17504/protocols.io.cdjs4m Copy
Authors: Isabel Gautreau
Group: New England Biolabs (NEB)
Proper citation: Isabel Gautreau 2020. NEBNext Ultra End Prep Mixture E7442. protocols.io dx.doi.org/10.17504/protocols.io.bpiumkew Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The following is a "typical" restriction endonuclease reaction. Please see the "guidelines" tab below for the NEB tips on optimizing restriction digests.
Proper citation: New England Biolabs 2014. Restriction Digest. protocols.io dx.doi.org/10.17504/protocols.io.chkt4v Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the protocol for the Q5® Site-Directed Mutagenesis Kit (E0554)
Proper citation: New England Biolabs 2014. Q5® Site-Directed Mutagenesis (E0554). protocols.io dx.doi.org/10.17504/protocols.io.cjpumm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. NEB Immunoprecip. Buffer. protocols.io dx.doi.org/10.17504/protocols.io.crbv2m Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is a generic PNGase F protocol with denaturing reaction conditions. It is appropriate for both P0704 and P0708.
Proper citation: New England Biolabs 2015. PNGase F Protocol, Denaturing Conditions. protocols.io dx.doi.org/10.17504/protocols.io.cqfvtm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the quick protocol for the Q5® Site-Directed Mutagenesis Kit (E0554)
Proper citation: New England Biolabs 2015. Q5® Site-Directed Mutagenesis Kit Quick Protocol (E0554). protocols.io dx.doi.org/10.17504/protocols.io.cpyvpv Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Q5 PCR Mixture for M0302. protocols.io dx.doi.org/10.17504/protocols.io.cqqvvv Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This PCR Protocol is for Taq DNA Polymerase with Standard Taq Buffer (M0273)
Proper citation: New England Biolabs 2015. PCR with Taq DNA Polymerase (M0273). protocols.io dx.doi.org/10.17504/protocols.io.ch7t9m Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the correct protocol if you are using the C2987H cells. If you are using the C2987I cells, please refer to this protocol.
Proper citation: New England Biolabs 2018. High Efficiency Transformation Protocol (C2987H). protocols.io dx.doi.org/10.17504/protocols.io.isxcefn Copy
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