Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
MojoSort™ Selection Kits Column Protocol - 3 Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7tvhnn6 | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody than with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-selection-kits-column-protocol-3/5006/ | 1 | 2019 | Sam Li 2019. MojoSort™ Selection Kits Column Protocol - 3. protocols.io https://dx.doi.org/10.17504/protocols.io.7tvhnn6 | 2021-04-15 09:15:36 | ||
|
MojoSort™ Human CD14+ Monocytes Isolation Kit Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7x9hpr6 | BioLegend | Product description and procedure summary: This kit is designed for the isolation of untouched CD14+CD16- monocytes from peripheral blood mononuclear cells (PBMCs). Target cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched cells are collected. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water.Scale up volumes if using 14mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-cd14--monocytes-isolation-kit-protocol/4351/ | 1 | 2019 | Sam Li 2019. MojoSort™ Human CD14+ Monocytes Isolation Kit Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7x9hpr6 | 2021-04-15 09:15:37 | ||
|
Propidium Iodide Cell Cycle Staining Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.bacyiaxw | BioLegend | BioLegend | https://www.biolegend.com/protocols/propidium-iodide-cell-cycle-staining-protocol/4303/ | 3 | 2019 | Sam Li 2019. Propidium Iodide Cell Cycle Staining Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bacyiaxw | 2021-04-15 09:15:09 | |||
|
MojoSort™ Mouse CD4 Nanobeads Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7wthpen | BioLegend | Product description and procedure summary:The cells targeted by the Nanobeads are either selected or depleted by incubating your sample with the directly conjugated magnetic particles. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. Scale up volumes if using 14mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-mouse-cd4-nanobeads-protocol/4314/ | 1 | 2019 | Sam Li 2019. MojoSort™ Mouse CD4 Nanobeads Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7wthpen | 2021-04-15 09:15:44 | ||
|
Anti-c-Myc Tag (9E10) Affinity Gel Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.97ih9ke | BioLegend | Catalog Number: 658502Storage Temperature: 2°C-8°CProduct Description: Anti-c-Myc Tag (9E10) Affinity Gel consists of anti-c-myc monoclonal antibody (clone 9E10), covalently immobilized onto 6% high density glyoxal agarose beads. The affinity resin can be used in affinity purifying and immunoprecipitation of c-Myc-tagged fusion protein.Binding Specificity: Mouse monoclonal antibody 9E10 recognizes the c-myc epitope N-EQKLSEEDL-C and is purified through Protein G chromatography. The antibody conjugated affinity resin can bind to epitope at N-terminal, C-terminal, and internal locations of a fusion protein. The binding capacity is greater than 0.5mg per ml of c-myc agarose resin.Reagent: Anti-c-Myc Agarose Affinity Gel is supplied as a 50% suspension in phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide.Precautions and Disclaimer: This product is for research use only. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.Storage/Stability: Anti-c-Myc Agarose Affinity Gel should be stored between 2°C and 8°C for maximum stability. The unopened product is stable for one year when stored as indicated. After use, the resin should be regenerated and stored in TBS or PBS buffer (pH 7.2) containing 0.09% sodium azide. | BioLegend | https://www.biolegend.com/protocols/anti-c-myc-tag-9e10-affinity-gel-protocol/4297/ | 2 | 2019 | Sam Li 2019. Anti-c-Myc Tag (9E10) Affinity Gel Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.97ih9ke | 2021-04-15 09:15:15 | ||
|
MojoSort™ Mouse Ig light chain κ Nanobeads Column Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.bbueinte | BioLegend | BioLegend MojoSort™ Nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing in academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads than with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/en-us/protocols/mojosort-mouse-ig-light-chain-k-nanobeads-column-protocol | 1 | 2020 | Sam Li 2020. MojoSort™ Mouse Ig light chain κ Nanobeads Column Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bbueinte | 2021-04-15 09:15:17 | ||
|
Cell Surface Flow Cytometry Staining of Whole Blood Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.babbiain | BioLegend | BioLegend | https://www.biolegend.com/protocols/cell-surface-flow-cytometry-staining-of-whole-blood/4240/ | 4 | 2019 | Sam Li 2019. Cell Surface Flow Cytometry Staining of Whole Blood. protocols.io https://dx.doi.org/10.17504/protocols.io.babbiain | 2021-04-15 09:15:17 | |||
|
MojoSort™ Selection Kits Column Protocol - 5 Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7u3hnyn | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-selection-kits-column-protocol-5/4769/ | 1 | 2019 | Sam Li 2019. MojoSort™ Selection Kits Column Protocol - 5. protocols.io https://dx.doi.org/10.17504/protocols.io.7u3hnyn | 2021-04-15 09:15:17 | ||
|
True-Nuclear™ Transcription Factor Staining Protocol for 5 mL Tubes Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.bacsiawe | BioLegend | BioLegend | https://www.biolegend.com/protocols/true-nuclear-transcription-factor-staining-protocol-for-5ml-tubes/4267/ | 4 | 2019 | Sam Li 2019. True-Nuclear™ Transcription Factor Staining Protocol for 5 mL Tubes. protocols.io https://dx.doi.org/10.17504/protocols.io.bacsiawe | 2021-04-15 09:15:15 | |||
|
Flex-T™ Tetramer and Cell Staining Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.babeiaje | BioLegend | Using UV-induced peptide exchange, MHC/peptide monomers can be generated with conditional Flex-T™ monomers that harbor peptides of interest in their binding grooves. These new MHC monomers are subsequently multimerized using streptavidin-fluorophore conjugates. The resulting Flex-T™ reagents can be used for staining antigen-specific T cells and flow cytometric analysis. In humans, the MHC molecules are called HLA (Human Leukocyte Antigen). | BioLegend | https://www.biolegend.com/protocols/flex-t-tetramer-preparation-and-flow-cytometry-staining-protocol/4251/ | 3 | 2019 | Sam Li 2019. Flex-T™ Tetramer and Cell Staining Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.babeiaje | 2021-04-15 09:15:19 | ||
|
Cell Surface Flow Cytometry Staining Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.baa9iah6 | BioLegend | BioLegend | https://www.biolegend.com/protocols/cell-surface-flow-cytometry-staining-protocol/4283/ | 4 | 2019 | Sam Li 2019. Cell Surface Flow Cytometry Staining Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.baa9iah6 | 2021-04-15 09:15:18 | |||
|
Immunofluorescent Staining of Foxp3 in Frozen Sections Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.97ch9iw | BioLegend | T regulatory cells (also known as Tregs or Regulatory T cells) are essential cells in the immune system that suppress immune responses of other cells, designed to limit excessive reactions and prevent autoimmunity. Tregs are characterized by the expression of CD4, CD25, and FOXP3, while lacking CD127. CD4+FOXP3+ regulatory T cells have been referred to as "naturally-occurring" regulatory T cells to distinguish them from "suppressor" T cell populations that are generated in vitro. While other variants of suppressive T cells do exist, such as CD8 suppressor cells, Th3 and Tr-1 cells, Tregs are classically defined as CD4+CD25+FOXP3+ cells. Here, we provide a protocol for immunofluorescent staining of FOXP3 in frozen sections, adapted from a protocol from Dr. Matthias Hardtke-Wolenski, Medizinische Hochschule Hannover. This protocol has been successfully used on mouse spleen, liver, and skin sections. | BioLegend | https://www.biolegend.com/protocols/immunofluorescent-staining-of-foxp3-in-frozen-sections/4254/ | 3 | 2019 | Sam Li 2019. Immunofluorescent Staining of Foxp3 in Frozen Sections. protocols.io https://dx.doi.org/10.17504/protocols.io.97ch9iw | 2021-04-15 09:15:22 | ||
|
MojoSort™ Human anti-PE Nanobeads Column Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7aghibw | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-anti-pe-nanobeads-column-protocol/4761/ | 1 | 2019 | Sam Li 2019. MojoSort™ Human anti-PE Nanobeads Column Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7aghibw | 2021-04-15 09:15:23 | ||
|
Anti-Neu5Gc Antibody Kit Protocol - Flow Cytometry Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.baaqiadw | BioLegend | Anti-Neu5Gc may be used for staining cells prior to analysis by Flow Cytometry. This kit contains all the essential components needed to identify Neu5Gc on the surface of cells by flow cytometry.Use of the blocking agent (Neu5Gc Assay Blocking Solution) provided in the kit is essential, as commonly used blocking agents invariably contain serum, or serum components, that can either inhibit detection or introduce Neu5Gc contamination.Tissue culture-grown CHO-K1 cells can be used as a positive control, and human peripheral blood mononuclear cells serve as a negative control. Adherent tissue culture-grown cells should be released from the culture flasks by using 5-10mM EDTA for 10 minutes at room temperature. Other non-enzymatic methods, such as Accutase®, may be used. Immediately wash cells in blocking buffer that contains a lower concentration of EDTA, and resuspend cells in blocking buffer to determine cell numbers and viability.It is assumed that the user is familiar with the general principles and practices of Flow Cytometry. | BioLegend | https://www.biolegend.com/protocols/anti-neu5gc-antibody-kit-protocol-flow-cytometry/4264/ | 3 | 2019 | Sam Li 2019. Anti-Neu5Gc Antibody Kit Protocol - Flow Cytometry. protocols.io https://dx.doi.org/10.17504/protocols.io.baaqiadw | 2021-04-15 09:15:49 | ||
|
MojoSort™ Human CD14+ Monocyte Isolation Kit Column Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.bbwaipae | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody cocktail that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:2 to 1:10 for the antibody cocktail can be used. Dilutions ranging from 1:5 to 1:20 for the Streptavidin Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-cd14--monocyte-isolation-kit-column-protocol/4764/ | 2 | 2020 | Sam Li 2020. MojoSort™ Human CD14+ Monocyte Isolation Kit Column Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bbwaipae | 2021-04-15 09:15:25 | ||
|
MojoSort™ Human anti-APC Nanobeads Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7x2hpqe | BioLegend | Product description and procedure summary:Target cells are positively selected or depleted by incubating the sample with an anti-human APC conjugated antibody, followed by incubation with magnetic anti-APC Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. These are the APC+ cells, do not discard them if those are the cells of interest. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water.Scale up volumes if using 14 mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-anti-apc-nanobeads-protocol/4755/ | 1 | 2019 | Sam Li 2019. MojoSort™ Human anti-APC Nanobeads Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7x2hpqe | 2021-04-15 09:15:32 | ||
|
Cell Fixation and Permeabilization Protocol using 70% Ethanol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.bacziax6 | BioLegend | BioLegend | https://www.biolegend.com/protocols/cell-fixation-and-permeabilization-protocol-using-70-ethanol/4252/ | 3 | 2019 | Sam Li 2019. Cell Fixation and Permeabilization Protocol using 70% Ethanol. protocols.io https://dx.doi.org/10.17504/protocols.io.bacziax6 | 2021-04-15 09:15:50 | |||
|
MojoSort™ Streptavidin Nanobeads Column Protocol - Negative Selection Resource Report Resource Website |
Kelsey Miller, Sam Li | DOI:10.17504/protocols.io.693hh8n | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody cocktail that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:2 to 1:10 for the antibody cocktail can be used. Dilutions ranging from 1:5 to 1:20 for the Streptavidin Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend, BioLegend | https://www.biolegend.com/protocols/mojosort-streptavidin-nanobeads-column-protocol-negative-selection/4772/ | 2 | 2019 | Kelsey Miller, Sam Li 2019. MojoSort™ Streptavidin Nanobeads Column Protocol - Negative Selection. protocols.io https://dx.doi.org/10.17504/protocols.io.693hh8n | 2021-04-15 09:15:51 | ||
|
MojoSort™ Nanobeads Protocol - 1 Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7u5hny6 | BioLegend | Product description and procedure summary:The cells targeted by the Nanobeads are either selected or depleted by incubating your sample with the directly conjugated magnetic particles. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. Scale up volumes if using 14 mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-nanobeads-protocol-1/4603/ | 1 | 2019 | Sam Li 2019. MojoSort™ Nanobeads Protocol - 1. protocols.io https://dx.doi.org/10.17504/protocols.io.7u5hny6 | 2021-04-15 09:15:40 | ||
|
TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3' Reagent Kit v3 3.1 Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.8aahsae | BioLegend | Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. TotalSeq™-B antibodies are designed to be used with 10x Single Cell 3’ Reagent Kit v3 with Feature Barcoding. The adjusted protocol below is for customers who are utilizing TotalSeq™-A antibodies with the v3 kit instead. Note: Step 4 from the 10x Genomics user guide document number CG000185, Rev B only applies to the cDNA library and NOT to the TotalSeq™-A library. Please read the entire protocol below and the 10x Genomics user guide for the Chromium Single Cell 3ʹ Reagent Kits v3 with Feature Barcoding technology for Cell Surface Protein before starting the experiments. 10x Genomics user guide document number CG000185, Rev B. Commonly used abbreviations:ADT: Antibody derived tags, the oligo sequence conjugated to regular TotalSeq™-A antibodiesHTO: Hashtag oligonucleotides, the oligo sequence conjugated to TotalSeq™-A Hashtag antibodies | BioLegend | https://www.biolegend.com/protocols/totalseq-a-antibodies-and-cell-hashing-with-10x-single-cell-3-reagent-kit-v3-31-protocol/5010/ | 1 | 2019 | Sam Li 2019. TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3' Reagent Kit v3 3.1 Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.8aahsae | 2021-04-15 09:15:40 |
Can't find your Protocol?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific protocol and you know the DOI of the protocol already, it's easier to enter a DOI to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your protocol in the search results, please help us by adding it into the system — it's easy. Create and publish your protocols at Protocols.io.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
