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Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the protocol for Protein Expression Using BL21(DE3) Competent E. coli (C2527). For large scale, please follow this protocol variant.
Proper citation: New England Biolabs 2014. Protein Expression Using BL21(DE3) (C2527). protocols.io dx.doi.org/10.17504/protocols.io.cjqumv Copy
Authors: New England Biolabs
Group: Grupo de Estudos de Leveduras Cervejeiras
Proper citation: New England Biolabs 2018. SOB Media. protocols.io dx.doi.org/10.17504/protocols.io.pnfdmbn Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The BioBrick® Assembly Kit was developed in partnership with Ginkgo BioWorks. What follows is an abbreviated set of protocols for the use of the BioBrick® Assembly Kit (to assemble an Upstream Part with a Downstream Part into a Destination Plasmid). For more details and for technical questions, please see here.
Proper citation: New England Biolabs 2015. Digestion for BioBrick Assembly Kit (E0546). protocols.io dx.doi.org/10.17504/protocols.io.cphvj5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is a protocol for a typical DNase I Reaction, using the M0303 RNase-free DNase I.
Proper citation: New England Biolabs 2015. A Typical DNase I Reaction (M0303). protocols.io dx.doi.org/10.17504/protocols.io.cqevtd Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Digestion Mixture for M0302. protocols.io dx.doi.org/10.17504/protocols.io.cqrvv5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Protocol for Dephosphorylation of 5´-ends of DNA using rSAP (M0371)
Proper citation: New England Biolabs 2015. Dephosphorylation of 5´-ends of DNA using rSAP (M0371). protocols.io dx.doi.org/10.17504/protocols.io.crkv4v Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina contains the enzymes and buffers required to convert a broad range of input amounts of RNA into high quality directional (strand-specific) libraries for next-generation sequencing on the Illumina platform. The fast, user-friendly workflow has minimal hands-on time and is compatible with poly(A) mRNA enrichment and rRNA depletion methods.
Proper citation: New England Biolabs 2018. Protocol for use with rRNA Depleted FFPE RNA and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765). protocols.io dx.doi.org/10.17504/protocols.io.ubaesie Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the PCR protocol for Phusion® High-Fidelity DNA Polymerase (M0530)
Proper citation: New England Biolabs 2015. PCR with Phusion® Polymerase (M0530). protocols.io dx.doi.org/10.17504/protocols.io.cdjs4m Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The following is a "typical" restriction endonuclease reaction. Please see the "guidelines" tab below for the NEB tips on optimizing restriction digests.
Proper citation: New England Biolabs 2014. Restriction Digest. protocols.io dx.doi.org/10.17504/protocols.io.chkt4v Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the protocol for the Q5® Site-Directed Mutagenesis Kit (E0554)
Proper citation: New England Biolabs 2014. Q5® Site-Directed Mutagenesis (E0554). protocols.io dx.doi.org/10.17504/protocols.io.cjpumm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. NEB Immunoprecip. Buffer. protocols.io dx.doi.org/10.17504/protocols.io.crbv2m Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is a generic PNGase F protocol with denaturing reaction conditions. It is appropriate for both P0704 and P0708.
Proper citation: New England Biolabs 2015. PNGase F Protocol, Denaturing Conditions. protocols.io dx.doi.org/10.17504/protocols.io.cqfvtm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the quick protocol for the Q5® Site-Directed Mutagenesis Kit (E0554)
Proper citation: New England Biolabs 2015. Q5® Site-Directed Mutagenesis Kit Quick Protocol (E0554). protocols.io dx.doi.org/10.17504/protocols.io.cpyvpv Copy
Authors: New England Biolabs, Menna Teffera
Summary: This module is part of the Ultra™ II workflow, and is optimized for use with the NEBNext®Ultra II End Repair/dA-Tailing Module (NEB #E7546), for Illumina®-compatible library construction.The NEBNext Ultra II Ligation Module is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546) or the NEBNext Ultra II FS DNA Module (NEB #E7810).
Proper citation: New England Biolabs, Menna Teffera 2019. NEBNext Ultra II Ligation Module (NEB # E7595) for NEBNext Ultra II End Repair/dA Tailing Module (NEB #E7546). protocols.io dx.doi.org/10.17504/protocols.io.4nqgvdw Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Q5 PCR Mixture for M0302. protocols.io dx.doi.org/10.17504/protocols.io.cqqvvv Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This PCR Protocol is for Taq DNA Polymerase with Standard Taq Buffer (M0273)
Proper citation: New England Biolabs 2015. PCR with Taq DNA Polymerase (M0273). protocols.io dx.doi.org/10.17504/protocols.io.ch7t9m Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. BioBrick E0546 Upstream Reaction. protocols.io dx.doi.org/10.17504/protocols.io.cpivkd Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. T4 PNK Reaction. protocols.io dx.doi.org/10.17504/protocols.io.cpevjd Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Ultra II FS DNA Module contains the enzymes and buffers required to convert a broad range of input amounts of intact DNA into fragmented DNA with 5´ phosphorylated 3´ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595) and with the NEBNext Ultra II Q5 Master Mix (NEB #M0544) if amplification is required. The fast, user-friendly workflow has minimal hands on time.Note: The Ultra II FS Module is not compatible with bisulfite conversion workflows
Proper citation: New England Biolabs 2018. NEBNext Ultra II FS DNA Module E7810. protocols.io dx.doi.org/10.17504/protocols.io.n34dgqw Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Mixture for M0491 Q5 PCR. protocols.io dx.doi.org/10.17504/protocols.io.cieubd Copy
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