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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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U Mass - Glucose Tolerance Test Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xxafpie | Mouse Metabolic Phenotyping Centers | Glucose tolerance test measures systemic clearance of glucose following an intraperitoneal bolus injection of 20% dextrose. This experiment measures insulin sensitivity in awake mice assuming that there are no alterations in the animal’s pancreatic -cell function and insulin secretion. Insulin sensitivity is altered in obese mice. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=141&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Glucose Tolerance Test. protocols.io https://dx.doi.org/10.17504/protocols.io.xxafpie | 2021-04-15 09:16:55 | ||
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U Mass - Cholesterol (HDL) Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xt6fnre | Mouse Metabolic Phenotyping Centers | Summary:This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of HDL-cholesterol reflect cholesterol metabolism and are associated with cardiovascular disease. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=170&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Cholesterol (HDL). protocols.io https://dx.doi.org/10.17504/protocols.io.xt6fnre | 2021-04-15 09:16:39 | ||
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U Mass - Glucagon Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xw3fpgn | Mouse Metabolic Phenotyping Centers | This experiment provides the quantification of multiple hormones using multiplexed-Luminex technology based on beads containing specific antibodies. Sample matrices include plasma, serum, tissue culture supernatants, and tissue or cell lysates. Serum glucagon levels reflect glucagon secretion and pancreatic α-cell function. Serum glucagon levels regulate hepatic glucose production and glucose homeostasis. Serum glucagon levels are altered in obesity, insulin resistance, and counterregulatory stress response. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=173&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Glucagon. protocols.io https://dx.doi.org/10.17504/protocols.io.xw3fpgn | 2021-04-15 09:16:45 | ||
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U Mass - Basal glucose metabolism Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xr8fm9w | Mouse Metabolic Phenotyping Centers | Summary: Whole body glucose turnover and hepatic glucose production rates are measured at basal state using an intravenous infusion of labeled glucose in awake mice. Whole body glucose turnover and hepatic glucose production regulate basal glucose levels and are altered in obesity. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=137&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Basal glucose metabolism. protocols.io https://dx.doi.org/10.17504/protocols.io.xr8fm9w | 2021-04-15 09:17:07 | ||
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U Mass - Chronic/acute phloridzin treatment Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xujfnun | Mouse Metabolic Phenotyping Centers | Summary:Phloridzin is a potent inhibitor of renal glucose reabsorption and may be used to lower serum glucose levels. Phloridzin may be administered chronically to induce glycosuria and lower hyperglycemia in diabetic mice. Phloridzin may be administered acutely to reduce serum glucose levels for experiments in diabetic mice. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=154&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Chronic/acute phloridzin treatment. protocols.io https://dx.doi.org/10.17504/protocols.io.xujfnun | 2021-04-15 09:14:23 | ||
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U Mass - Chronic drug delivery Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xuefnte | Mouse Metabolic Phenotyping Centers | A subcutaneous or intraperitoneal implantation of Alzet osmotic pump is used to chronically administer selected drug in mice. Chronic drug delivery may be used to examine intermediate to long-term effects of selected drug on obesity, insulin resistance, and metabolism. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=151&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Chronic drug delivery. protocols.io https://dx.doi.org/10.17504/protocols.io.xuefnte | 2021-04-15 09:14:27 | ||
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U Mass - Glucose Tolerance Test with insulin secretion Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xxbfpin | Mouse Metabolic Phenotyping Centers | Summary:Glucose tolerance test with insulin secretion measures systemic clearance of glucose and systemic appearance of insulin following an intraperitoneal bolus injection of 20% dextrose. This experiment measures insulin sensitivity and insulin secretion (pancreatic -cell function) in awake mice assuming that there are no alterations in systemic insulin clearance. Insulin sensitivity and pancreatic -cell function are altered in obese mice. | RRID:AB_2792981 | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=142&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Glucose Tolerance Test with insulin secretion. protocols.io https://dx.doi.org/10.17504/protocols.io.xxbfpin | 2021-04-15 09:14:37 | |
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U Mass - Non-esterified fatty acids Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.x3hfqj6 | Mouse Metabolic Phenotyping Centers | This experiment measures serum and plasma concentrations of non-esterified fatty acids using a 96-well kit. The experiment involves a coupled reaction to measure non-esterified fatty acids (NEFA) which ultimately forms a purple product that absorbs light at 550nm. This allows the concentration of NEFA to be determined from the optical density measured at 540~550nm. Serum fatty acids levels reflect systemic lipid metabolism, lipid digestion/absorption, and lipid clearance. Serum fatty acids levels are altered in obesity, insulin resistance, and type 2 diabetes. | University of Massachusetts | http://mmpc.org/shared/document.aspx?id=168&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Non-esterified fatty acids. protocols.io https://dx.doi.org/10.17504/protocols.io.x3hfqj6 | 2021-04-15 09:14:36 | ||
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U Mass - Total protein Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.x5gfq3w | Mouse Metabolic Phenotyping Centers | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of total protein reflect systemic protein metabolism. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=186&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Total protein. protocols.io https://dx.doi.org/10.17504/protocols.io.x5gfq3w | 2021-04-15 09:14:37 | ||
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U Mass - Triglyceride Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.x5pfq5n | Mouse Metabolic Phenotyping Centers, Metabolomics Protocols & Workflows | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Triglyceride levels may be measured in serum, plasma, and tissue samples. Serum and tissue triglyceride levels are affected by alterations in systemic lipid metabolism, lipid digestion/absorption, and lipid clearance. Serum and tissue triglyceride levels are altered in obesity, insulin resistance, type 2 diabetes, alcoholic steatohepatitis, non-alcoholic fatty liver disease, and non-alcoholic steatohepatitis. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=167&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Triglyceride. protocols.io https://dx.doi.org/10.17504/protocols.io.x5pfq5n | 2021-04-15 09:14:37 | ||
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U Mass - Amylase Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xq4fmyw | Mouse Metabolic Phenotyping Centers | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=175&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Amylase. protocols.io https://dx.doi.org/10.17504/protocols.io.xq4fmyw | 2021-04-15 09:14:43 | ||
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U Mass - Uric Acid Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.x5sfq6e | Mouse Metabolic Phenotyping Centers | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum uric acid levels are affected by alterations in systemic protein and nitrogen metabolism. Serum uric acid levels are altered in kidney failure and renal complications of diabetes. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=188&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Uric Acid. protocols.io https://dx.doi.org/10.17504/protocols.io.x5sfq6e | 2021-04-15 09:14:45 | ||
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U Mass - Alanine Transferase Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xpmfmk6 | Mouse Metabolic Phenotyping Centers | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum alanine transferase levels reflect liver function. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=180&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Alanine Transferase. protocols.io https://dx.doi.org/10.17504/protocols.io.xpmfmk6 | 2021-04-15 09:15:13 | ||
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U Mass - Leptin Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.x23fqgn | Mouse Metabolic Phenotyping Centers | This experiment provides the quantification of multiple hormones using multiplexed-Luminex technology based on beads containing specific antibodies. Sample matrices include plasma, serum, tissue culture supernatants, and tissue or cell lysates. Leptin is an adipocyte-derived hormone (adipokine) that regulates feeding behavior (hunger/satiety). Serum leptin levels are altered in obesity, insulin resistance, and type 2 diabetes. | University of Massachusetts | http://mmpc.org/shared/document.aspx?id=164&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Leptin. protocols.io https://dx.doi.org/10.17504/protocols.io.x23fqgn | 2021-04-15 09:15:13 | ||
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U Mass - Organ-specific glucose uptake Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.x4cfqsw | Mouse Metabolic Phenotyping Centers | Glucose uptake in individual organs can be measured using a bolus injection of 2-deoxy-D-[1-14C] glucose, a non-metabolizable glucose analog, and by determining labeled metabolite levels in select tissues. Insulin resistance is characterized by reduced glucose metabolism and develops in obese mice. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=138&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Organ-specific glucose uptake. protocols.io https://dx.doi.org/10.17504/protocols.io.x4cfqsw | 2021-04-15 09:15:18 | ||
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U Mass - Surgery – jugular vein cannulation Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.x5cfq2w | Mouse Metabolic Phenotyping Centers | Summary:Chronic indwelling catheter is placed in the jugular vein for intravenous infusion and injection of drug, hormones, and metabolites. Surival surgery is performed in anesthetized mice, and mice recover from surgery after 4~5 days. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=156&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Surgery – jugular vein cannulation. protocols.io https://dx.doi.org/10.17504/protocols.io.x5cfq2w | 2021-04-15 09:15:21 | ||
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U Mass - C-reactive Peptide Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xt5fnq6 | Mouse Metabolic Phenotyping Centers | Summary:This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of C-reactive peptide reflect systemic inflammation and may be altered in obesity. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=185&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - C-reactive Peptide. protocols.io https://dx.doi.org/10.17504/protocols.io.xt5fnq6 | 2021-04-15 09:15:29 | ||
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U Mass - Lipase Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.x3bfqin | Mouse Metabolic Phenotyping Centers | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Lipase is a gastrointestinal enzyme that processes fat/lipid digestion. | University of Massachusetts | http://mmpc.org/shared/document.aspx?id=174&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Lipase. protocols.io https://dx.doi.org/10.17504/protocols.io.x3bfqin | 2021-04-15 09:15:53 | ||
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U Mass - Hyperglycemic clamp Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xzafp2e | Mouse Metabolic Phenotyping Centers | Summary:Hyperglycemic clamp measures insulin secretion and pancreatic -cell function in awake mice. Islet function is affected by obesity and insulin resistance, and abnormal islet function predisposes to the development of type 2 diabetes. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=139&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Hyperglycemic clamp. protocols.io https://dx.doi.org/10.17504/protocols.io.xzafp2e | 2021-04-15 09:15:51 | ||
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U Mass - Lipid metabolism Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.x3gfqjw | Mouse Metabolic Phenotyping Centers | Lipid metabolism is estimated by measuring systemic clearance of [1-14C] palmitate following a bolus injection in awake mice. Lipid metabolism is altered in obese mice. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=145&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Lipid metabolism. protocols.io https://dx.doi.org/10.17504/protocols.io.x3gfqjw | 2021-04-15 09:15:44 |
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