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Authors: DAVID DUNIGAN AND IRINA AGARKOVA
Group: VERVE Net
Summary: For use in CviRI Purification From XZ-6E Virus Infected NC64A Chlorella.
Proper citation: DAVID DUNIGAN AND IRINA AGARKOVA 2016. 1X CviRI Assay Buffer. protocols.io dx.doi.org/10.17504/protocols.io.es2bege Copy
Authors: David Dunigan and Irina Agarkova
Group: VERVE Net
Proper citation: David Dunigan and Irina Agarkova 2016. Viral DNA Miniprep Procedure. protocols.io dx.doi.org/10.17504/protocols.io.eq6bdze Copy
Authors: DAVID DUNIGAN AND IRINA AGARKOVA
Group: VERVE Net
Summary: For use in CviRI Purification From XZ-6E Virus Infected NC64A Chlorella.
Proper citation: DAVID DUNIGAN AND IRINA AGARKOVA 2016. CviRI Storage Buffer. protocols.io dx.doi.org/10.17504/protocols.io.eszbef6 Copy
Authors: David Dunigan and Irina Agarkova
Group: VERVE Net
Proper citation: David Dunigan and Irina Agarkova 2016. Gradient Recipes. protocols.io dx.doi.org/10.17504/protocols.io.eufbetn Copy
Authors: DAVID DUNIGAN AND IRINA AGARKOVA
Group: VERVE Net
Summary: For use in Polyacrylamide Gel System For Electrophoresis Of Proteins.
Proper citation: DAVID DUNIGAN AND IRINA AGARKOVA 2016. Running gel buffer (10X). protocols.io dx.doi.org/10.17504/protocols.io.ercbd2w Copy
Authors: David Dunigan and Irina Agarkova
Group: VERVE Net
Proper citation: David Dunigan and Irina Agarkova 2016. CviJI Purification From IL-3A Virus Infected NC64A Chlorella. protocols.io dx.doi.org/10.17504/protocols.io.er3bd8n Copy
Authors: DAVID DUNIGAN AND IRINA AGARKOVA
Group: VERVE Net
Summary: For use in CviRI Purification From XZ-6E Virus Infected NC64A Chlorella.
Proper citation: DAVID DUNIGAN AND IRINA AGARKOVA 2016. CviRI Buffer B. protocols.io dx.doi.org/10.17504/protocols.io.esxbefn Copy
Authors: David Dunigan and Irina Agarkova
Group: VERVE Net
Summary: PBCV-1 is a large plaque virus (approximately 2-3 mm in diameter). The plaque size of the viruses reflects the length of the virus replication cycle. PBCV-1 has a life cycle of about 6-8 hours. When titering you should use plates that were poured no more than 1 day before the time of use. Variable plaque formation will result if fresh plates are not used. (This is similar to a phage phenomenon.) Pyrex petri dishes do give more uniform plaque formation versus the plastic, however this is not so critical as with slower growing NC64A viruses.
Proper citation: David Dunigan and Irina Agarkova 2016. PBCV-1 Virus Plaque Assay. protocols.io dx.doi.org/10.17504/protocols.io.estbeen Copy
Authors: David Dunigan and Irina Agarkova
Group: VERVE Net
Proper citation: David Dunigan and Irina Agarkova 2016. Formulation of FES Medium. protocols.io dx.doi.org/10.17504/protocols.io.eucbesw Copy
Authors: David Dunigan and Irina Agarkova
Group: VERVE Net
Proper citation: David Dunigan and Irina Agarkova 2016. DTT solution. protocols.io dx.doi.org/10.17504/protocols.io.erabd2e Copy
Authors: DAVID DUNIGAN AND IRINA AGARKOVA
Group: VERVE Net
Summary: For use in CviRI Purification From XZ-6E Virus Infected NC64A Chlorella.
Proper citation: DAVID DUNIGAN AND IRINA AGARKOVA 2016. CviRI Assay Conditions. protocols.io dx.doi.org/10.17504/protocols.io.es3begn Copy
Authors: David Dunigan and Irina Agarkova
Group: VERVE Net
Summary: Western blotting uses specific antibodies to identify proteins that have been separated based on size by gel electrophoresis. The immunoassay uses a membrane made of nitrocellulose or PVDF (polyvinylidene fluoride). The gel is placed next to the membrane and application of an electrical current induces the proteins to migrate from the gel to the membrane. The membrane can then be further processed with antibodies specific for the target of interest, and visualized using secondary antibodies and detection reagents.
Proper citation: David Dunigan and Irina Agarkova 2016. Definitive guide to western blot. protocols.io dx.doi.org/10.17504/protocols.io.eqwbdxe Copy
Authors: David Dunigan and Irina Agarkova
Group: VERVE Net
Proper citation: David Dunigan and Irina Agarkova 2016. Chlorella NC64A and Mictractinium Pbi virus Purification. protocols.io dx.doi.org/10.17504/protocols.io.etfbejn Copy
Authors: DAVID DUNIGAN AND IRINA AGARKOVA
Group: VERVE Net
Summary: For use in CviJI Purification From IL-3A Virus Infected NC64A Chlorella.
Proper citation: DAVID DUNIGAN AND IRINA AGARKOVA 2016. CviJI Storage Buffer. protocols.io dx.doi.org/10.17504/protocols.io.esnbede Copy
Authors: David Dunigan and Irina Agarkova
Group: VERVE Net
Proper citation: David Dunigan and Irina Agarkova 2016. Methyltransferase Assay. protocols.io dx.doi.org/10.17504/protocols.io.etjbekn Copy
Authors: DAVID DUNIGAN AND IRINA AGARKOVA
Group: VERVE Net
Summary: For use in Polyacrylamide Gel System For Electrophoresis Of Proteins.
Proper citation: DAVID DUNIGAN AND IRINA AGARKOVA 2016. Cracking buffer, 4X (10 mL). protocols.io dx.doi.org/10.17504/protocols.io.erdbd26 Copy
Authors: David Dunigan and Irina Agarkova
Group: VERVE Net
Summary: This medium is for growing Chlorella variabilis NC64A, Chlorella heliozoae SAG 3.83, and Coccomyxa C-169.
Proper citation: David Dunigan and Irina Agarkova 2016. Formulation of MBBM (Modified Bold's Basal Medium). protocols.io dx.doi.org/10.17504/protocols.io.etwbepe Copy
Authors: David Dunigan and Irina Agarkova
Group: VERVE Net
Proper citation: David Dunigan and Irina Agarkova 2016. Extraction of DNA from Virus. protocols.io dx.doi.org/10.17504/protocols.io.et4beqw Copy
Authors: David Dunigan and Irina Agarkova
Group: VERVE Net
Proper citation: David Dunigan and Irina Agarkova 2016. RNA Isolation from Viral-infected Chlorella NC64A. protocols.io dx.doi.org/10.17504/protocols.io.erqbd5w Copy
Authors: DAVID DUNIGAN AND IRINA AGARKOVA
Group: VERVE Net
Summary: For use in Preparation of Denaturing Agarose Gel for RNA Analysis.
Proper citation: DAVID DUNIGAN AND IRINA AGARKOVA 2016. 10X MOPS Buffer. protocols.io dx.doi.org/10.17504/protocols.io.etgbejw Copy
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