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Name Authors DOI Group Summary Associated Publications RRIDs used Affiliations External URL Version Publication Date Proper Citation Record Last Update
COVID Blood Processing for scRNAseq
 
Resource Report
Resource Website
Peter Szabo, Steven Wells, Peter A. Sims, Donna Farber DOI:10.17504/protocols.io.bjm6kk9e Human Cell Atlas Method Development Community, Coronavirus Method Development Community This protocol describes the isolation of lymphocytes and pan-mononuclear cells from human whole blood for scRNAseq analysis. Columbia University, Columbia University, Columbia University, Columbia University 1 2020 Peter Szabo, Steven Wells, Peter A. Sims, Donna Farber 2020. COVID Blood Processing for scRNAseq. protocols.io https://dx.doi.org/10.17504/protocols.io.bjm6kk9e 2021-04-15 09:17:02
Amplicon clean-up using SPRI beads
 
Resource Report
Resource Website
Muhammad Faisal, Olin Silander, Nikki Freed DOI:10.17504/protocols.io.bfwpjpdn Coronavirus Method Development Community Massey University, Massey University, Massey University 1 2020 Muhammad Faisal, Olin Silander, Nikki Freed 2020. Amplicon clean-up using SPRI beads. protocols.io https://dx.doi.org/10.17504/protocols.io.bfwpjpdn 2021-04-15 09:17:04
Pooled sample testing (VTM/UTM) for SARS-CoV-2 using Magnetic Nanotrap® particles for direct RNA extraction.
 
Resource Report
Resource Website
Anurag Patnaik, Ben Lepene, Robert Barclay Coronavirus Method Development Community, Nanotrap Applications Ceres Nanosciences, inc., Ceres Nanosciences, inc., Ceres Nanosciences, inc. 1 2020 Anurag Patnaik, Ben Lepene, Robert Barclay 2020. Pooled sample testing (VTM/UTM) for SARS-CoV-2 using Magnetic Nanotrap® particles for direct RNA extraction.. protocols.io https:// 2021-04-15 09:17:06
Study Design (Part 3 of Phase 3 study of Vaccine Candidate for COVID-19)
 
Resource Report
Resource Website
Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores DOI:10.17504/protocols.io.bj5wkq7e Coronavirus Method Development Community This is Part 3 of "Phase 3 randomized, double-blinded, placebo-controlled trial to evaluate the safety, immunogenicity, and efficacy of Vaccine Candidate against COVID-19 in adults > 18 years of age"This generic Phase 3 protocol was developed by the PATH team with support of the Bill and Melinda Gates Foundation. The aim of the collection is to share recommended best practices in designing and implementing a Phase 3 study of a COVID-19 vaccine candidate. As Phase 3 trials of different Vaccine Candidates proceed around the world, following the same protocols will ensure consistency and comparability of the Phase 3 trial results.Please note that this is an evolving document, to be versioned and updated, based on community feedback and new data. Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington), Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington), Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington), Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington) 1 2020 Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores 2020. Study Design (Part 3 of Phase 3 study of Vaccine Candidate for COVID-19). protocols.io https://dx.doi.org/10.17504/protocols.io.bj5wkq7e 2021-04-15 09:17:07
Safety Assessment and Reporting (Part 7 of Phase 3 study of Vaccine Candidate for COVID-19)
 
Resource Report
Resource Website
Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores DOI:10.17504/protocols.io.bj52kq8e Coronavirus Method Development Community, PATH This is Part 7 of "Phase 3 randomized, double-blinded, placebo-controlled trial to evaluate the safety, immunogenicity, and efficacy of Vaccine Candidate against COVID-19 in adults > 18 years of age"This generic Phase 3 protocol was developed by the PATH team with support of the Bill and Melinda Gates Foundation. The aim of the collection is to share recommended best practices in designing and implementing a Phase 3 study of a COVID-19 vaccine candidate. As Phase 3 trials of different Vaccine Candidates proceed around the world, following the same protocols will ensure consistency and comparability of the Phase 3 trial results.Please note that this is an evolving document, to be versioned and updated, based on community feedback and new data. Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington), Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington), Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington), Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington) 1 2020 Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores 2020. Safety Assessment and Reporting (Part 7 of Phase 3 study of Vaccine Candidate for COVID-19). protocols.io https://dx.doi.org/10.17504/protocols.io.bj52kq8e 2021-04-15 09:17:10
Viral Sequencing, from Gunk to Graph (Two-step, strand-switching)
 
Resource Report
Resource Website
David Eccles DOI:10.17504/protocols.io.bebujanw Coronavirus Method Development Community This is a fast "gunk to graph" protocol for analysing viral RNA from nasopharyngeal swabs. The approach involves swab lysis and inactivation at the point of sampling, uses a cellulose binding / wash protocol to reduce extraction cost, incorporates sample-specific barcodes during first-strand synthesis, nanopore rapid-attachment primers during PCR amplification, and nanopore sequencing with parallel RAMPART analysis for fast assembly and phylogenetics.An alternative protocol using both forward and reverse primers can be found here. Malaghan Institute of Medical Research (NZ) 3 2020 David Eccles 2020. Viral Sequencing, from Gunk to Graph (Two-step, strand-switching). protocols.io https://dx.doi.org/10.17504/protocols.io.bebujanw 2021-04-15 09:17:08

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