Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
HiFi Gibson Assembly (Protocol for the NEBuilder® HiFi DNA Assembly Master Mix) Resource Report Resource Website |
Alba Balletbó | 10.17504/protocols.io.7kchksw | iGEM Wageningen 2019 | This is the protocol for DNA Assembly using the NEBuilder® HiFi DNA Assembly Master Mix. | Wageningen University | 1 | 2019 | Alba Balletbó 2019. HiFi Gibson Assembly (Protocol for the NEBuilder® HiFi DNA Assembly Master Mix). protocols.io dx.doi.org/10.17504/protocols.io.7kchksw | 2021-03-29 03:11:00 | |||
|
Crosslinking Immunoprecipitation Beads Resource Report Resource Website |
Bryon Drown, Caroline DeHart, Kelleher Research Group | 10.17504/protocols.io.bdhki34w | Immunoprecipitation with non-covalent capture of antibodies is a rapid and effective method for enriching samples with desired antigens. Interference by the antibody in down-stream analysis of intact proteins by mass spectrometry can be eliminated by covalent linkage of antibodies to magnetic beads. While several amine-reactive bifunctional linkers have been used for this application, dimethyl pimelimidate (DMP) are particularly advantageous due to its water-solubility, stability of resulting amidines at low pH, and retention of positive charge at reaction sites. By cross-linking to Protein A/G magnetic beads, a greater portion of antibodies are correctly oriented and able to engage their antigen. Cross-linking antibodies to Protein A/G beads with DMP is a quick and effective method that mitigates interference from heavy and light chain IgG in down-stream mass spectrometry applications. | Northwestern University, Northwestern University, Northwestern University | 1 | 2021 | Bryon Drown, Caroline DeHart, Kelleher Research Group 2021. Crosslinking Immunoprecipitation Beads. protocols.io dx.doi.org/10.17504/protocols.io.bdhki34w | 2021-03-29 03:11:00 | ||||
|
Immunohistochemical Expression of FAK and PCNA in Odontogenic Keratocyst, Orthokeratinized Odontogenic Cyst, Dentigerous Cyst and Glandular Odontogenic Cyst (In-vitro study) Resource Report Resource Website |
Seham Hazem El-Ayouti, Heba Mahmoud Dahmoush, Hatem Wael Amer | 10.17504/protocols.io.t5teq6n | Assistant Lecturer at Oral and Maxillofacial Pathology Department, Professor of Oral and Maxillofacial Pathology, Faculty of Dentistry, Cairo University, Lecturer of Oral and Maxillofacial Pathology, Faculty of Dentistry, Cairo University | 1 | 2018 | Seham Hazem El-Ayouti, Heba Mahmoud Dahmoush, Hatem Wael Amer 2018. Immunohistochemical Expression of FAK and PCNA in Odontogenic Keratocyst, Orthokeratinized Odontogenic Cyst, Dentigerous Cyst and Glandular Odontogenic Cyst (In-vitro study). protocols.io dx.doi.org/10.17504/protocols.io.t5teq6n | 2021-03-29 03:11:00 | |||||
|
PCSK9 gRNA amplification template PCR Resource Report Resource Website |
Noreen Wauford | 10.17504/protocols.io.e86bhze | protocols.io | 2 | 2016 | Noreen Wauford 2016. PCSK9 gRNA amplification template PCR. protocols.io dx.doi.org/10.17504/protocols.io.e86bhze | 2021-03-29 03:11:00 | |||||
|
Nipah virus real-time RT-PCR (NiV-TM2018) Resource Report Resource Website |
Ian Mackay, Judy Northill | 10.17504/protocols.io.rs5d6g6 | Public Health Virology, Forensic and Scientific Services | The protocol aims to specifically amplify Nipah viruses (NiV) and not other viruses.This is a modified version of a published assay. Modifications were to account for mismatches underneath oligo target sites.The assay targets the nucleoprotein (N) gene region and is designed as a qualitative test for investigating NiV infection of humans. | Public Health Virology, Forensic and Scientific Services, Public Health Virology, Forensic and Scientific Services | https://doi.org/10.3201/eid1802.111492 | 1 | 2019 | Ian Mackay, Judy Northill 2019. Nipah virus real-time RT-PCR (NiV-TM2018). protocols.io dx.doi.org/10.17504/protocols.io.rs5d6g6 | 2021-03-29 03:11:03 | ||
|
Nuclei-seq Resource Report Resource Website |
Mandy Xu, Troy Ketela | 10.17504/protocols.io.8b8hsrw | Human Cell Atlas Method Development Community, CZI START Project | This protocol has been developed by the Princess Margaret Genomics Centre specifically for Nuc-Seq, both at the bulk and single-cell levels. | Princess Margaret Genomic Centre, Princess Margaret Genomic Centre | 1 | 2019 | Mandy Xu, Troy Ketela 2019. Nuclei-seq. protocols.io dx.doi.org/10.17504/protocols.io.8b8hsrw | 2021-03-29 03:11:00 | |||
|
Determination of Glomerular Filtration Rate in Conscious Mice using FITC-inulin Resource Report Resource Website |
Zhonghua Qi, Matthew D. Breyer | 10.17504/protocols.io.3fngjme | Diabetic Complications Consortium | This protocol describes the procedures for estimating glomerular filtration rate in conscious mice based on Fluorescein Isothiocyanate-inulin (FITC-inulin) clearance following a single bolus intravenous injection. Diabetic Complication:Reference:1. Hem, A., Smith, A.J., and Solberg, P. 1998. Saphenous vein puncture for blood sampling of the mouse, rat, hamster, gerbil, guinea pig, ferret and mink. Lab Anim 32:364-368. 2. Sturgeon, C., Sam, A.D., 2nd, and Law, W.R. 1998. Rapid determination of glomerular filtration rate by single-bolus inulin: a comparison of estimation analyses. J Appl Physiol 84:2154-2162. 3. Qi, Z., Whitt, I., Mehta, A., Jin, J., Zhao, M., Harris, R.C., Fogo, A.B., and Breyer, M.D. 2003. Serial Determination of Glomerular Filtration Rate in Conscious Mice Using FITC-Inulin Clearance. Am J Physiol Renal Physiol. | Vanderbilt University, Eli Lilly Company | https://www.diacomp.org/shared/document.aspx?id=28&docType=Protocol | 1 | 2019 | Zhonghua Qi, Matthew D. Breyer 2019. Determination of Glomerular Filtration Rate in Conscious Mice using FITC-inulin. protocols.io dx.doi.org/10.17504/protocols.io.3fngjme | 2021-03-29 03:11:00 | ||
|
The method to measure one side surface area of the seaweed Resource Report Resource Website |
Xu Min | 10.17504/protocols.io.m6yc9fw | Institute of Oceanology, Chinese Academy of Sciences, Qingdao | 1 | 2018 | Xu Min 2018. The method to measure one side surface area of the seaweed. protocols.io dx.doi.org/10.17504/protocols.io.m6yc9fw | 2021-03-29 03:11:01 | |||||
|
Quick Fungal DNA extraction from colonies on plates Resource Report Resource Website |
Johannes Debler | 10.17504/protocols.io.snzedf6 | Adapted from Chi, M. H., Park, S. Y., & Lee, Y. H. (2009).Colony PCR on fungal colonies grown on plates does not work as well as it does for bacteria (it usually doesn't work at all). DNA therefore needs to be extracted first. As this DNA will only be used as a PCR template to check for presence / absence of individual genes I am not too concerend about high molecular weight or purity.This protocol is quick and can be used to process several samples at the same time. | Curtin University | 1 | 2018 | Johannes Debler 2018. Quick Fungal DNA extraction from colonies on plates. protocols.io dx.doi.org/10.17504/protocols.io.snzedf6 | 2021-03-29 03:11:06 | ||||
|
During data acquisition Resource Report Resource Website |
Lukas Snoek, Tinka Beemsterboer | 10.17504/protocols.io.nz2df8e | Spinoza Centre, REC-L | This protocol lists all the steps necessary to run your MRI experiment/data acquisition safely and in a way that yields high-quality data. Moreover, if you use the centre's QC/preprocessing service, it lists the steps necessary to make sure we can convert the data into BIDS and run them through the QC/preprocessing pipelines. | Spinoza Centre, REC-L, Spinoza Centre, REC-L | 4 | 2018 | Lukas Snoek, Tinka Beemsterboer 2018. During data acquisition. protocols.io dx.doi.org/10.17504/protocols.io.nz2df8e | 2021-03-29 03:11:01 | |||
|
Toehold switch assembly Resource Report Resource Website |
Junyan Qian | 10.17504/protocols.io.72bhqan | iGEM EPFL | General toehold assemble method using NEB Q5 High-Fidelity 2x Master Mix. Here we took Boir Noir toeholds as an example, it has four candidates we wanted to test. In order to have a higher yield, run 5 reactions for each candidates. | EPFL | 1 | 2019 | Junyan Qian 2019. Toehold switch assembly. protocols.io dx.doi.org/10.17504/protocols.io.72bhqan | 2021-03-29 03:11:00 | |||
|
30% Sucrose for Cryoprotection of brains after Perfusions Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.bg5ujy6w | BICCN, Allen Institute for Brain Science | This protocol is used to prepare 30% Sucrose Solution in 0.1M phosphate buffer. 30% Sucrose in 0.1M phosphate buffer is used for the cryoprotection of mice brains post perfusion.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 2 | 2020 | Allen Institute for Brain Science 2020. 30% Sucrose for Cryoprotection of brains after Perfusions. protocols.io dx.doi.org/10.17504/protocols.io.bg5ujy6w | 2021-03-29 03:11:06 | |||
|
CUT&Tag with Drosophila tissues Resource Report Resource Website |
Kami Ahmad, Steven Henikoff | 10.17504/protocols.io.bnx5mfq6 | This is a modification of the Benchtop CUT&Tag method (dx.doi.org/10.17504/protocols.io.bcuhiwt6) for epigenomic profiling of histone modifications and chromatin proteins in dissected Drosophila tissues. Intact unfixed tissues are permeabilized and incubated under conditions where a factor-specific antibody can infiltrate and bind a chromatin protein in situ. This antibody is then decorated with a secondary antibody, and this is used to tether a protein AG-Tn5 fusion protein loaded with sequencing adapters. Upon transposome activation by adding Mg2+ ions, DNA is tagmented around the chromatin binding site. Here, dissected imaginal discs are coated with magnetic beads for handling, and about 5 imaginal discs provides high-quality data for histone modifications or for chromatin factor for which high-quality antibodies are available. The protocol is scaled for 8 samples. Performing CUT&Tag takes 2 days from larvae to sequencing-ready libraries. | Fred Hutchinson Cancer Research Center, Fred Hutchinson Cancer Research Center | 1 | 2020 | Kami Ahmad, Steven Henikoff 2020. CUT&Tag with Drosophila tissues. protocols.io dx.doi.org/10.17504/protocols.io.bnx5mfq6 | 2021-03-29 03:11:01 | ||||
|
Soil extract for algal media Resource Report Resource Website |
Roscoff Culture Collection | 10.17504/protocols.io.s28eghw | Roscoff Culture Collection | How to prepare soil extract | Station Biologique, CNRS-Sorbonne Université, Roscoff France | 1 | 2019 | Roscoff Culture Collection 2019. Soil extract for algal media. protocols.io dx.doi.org/10.17504/protocols.io.s28eghw | 2021-03-29 03:11:02 | |||
|
Molecular Phenotype Distribution of Single Rat ICN Neurons - Heart B Resource Report Resource Website |
Shaina Robbins, Alison Moss, Sean Nieves | 10.17504/protocols.io.w56fg9e | SPARC | This collection of protocols were used to obtain the current data for the Blackfynn Dataset Molecular Phenotype Distribution of Single Rate ICN Neurons, which we refer to as Rat Heart B. | Thomas Jefferson University, Thomas Jefferson University, Thomas Jefferson University | 1 | 2020 | Shaina Robbins, Alison Moss, Sean Nieves 2020. Molecular Phenotype Distribution of Single Rat ICN Neurons - Heart B. protocols.io dx.doi.org/10.17504/protocols.io.w56fg9e | 2021-03-29 03:11:03 | |||
|
Direct ELISA for investigating the binding of recombinant Protein-LA to immunoglobulins. Resource Report Resource Website |
Angel Justiz-Vaillant, Monica F. Smikle | 10.17504/protocols.io.bjxukpnw | University of the West Indies, [email protected] | Referencesst protein LA (SpLA) is an immunoglobulin-binding protein that interacts with the Fab and Fc regions of many mammalian immunoglobulins [1].References1.Vaillant AJ, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. (2013) Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 4: 175. doi:10.4172/2155-9872.1000175 | University of the West Indies St. Augustine, University of the West Indies. Mona Campus | 1 | 2020 | Angel Justiz-Vaillant, Monica F. Smikle 2020. Direct ELISA for investigating the binding of recombinant Protein-LA to immunoglobulins.. protocols.io dx.doi.org/10.17504/protocols.io.bjxukpnw | 2021-03-29 03:11:03 | |||
|
Gel Extraction Resource Report Resource Website |
Nus Igem | 10.17504/protocols.io.7pthmnn | National University of Singapore | 1 | 2019 | Nus Igem 2019. Gel Extraction. protocols.io dx.doi.org/10.17504/protocols.io.7pthmnn | 2021-03-29 03:11:02 | |||||
|
Direct ELISA for investigating the binding of Protein-A to immunoglobulins. Resource Report Resource Website |
Angel Justiz-Vaillant, Monica F. Smikle | 10.17504/protocols.io.bjxqkpmw | University of the West Indies, [email protected] | University of the West Indies St. Augustine, University of the West Indies. Mona Campus | 1 | 2020 | Angel Justiz-Vaillant, Monica F. Smikle 2020. Direct ELISA for investigating the binding of Protein-A to immunoglobulins. . protocols.io dx.doi.org/10.17504/protocols.io.bjxqkpmw | 2021-03-29 03:11:02 | ||||
|
Predation assessment on fake caterpillars and leaf sampling: Protocol for partner schools Resource Report Resource Website |
Bastien Castagneyrol, Elena Valdés-Correcher, Michèle Kaennel Dobbertin, Martin Gossner | 10.17504/protocols.io.42pgydn | The 'Oak bodyguards' citizen science project aimed to assess the effects of climate on two key biotic interactions occurring widely in natural and anthropized ecosystems, i.e., the top-down and bottom-up forces controlling insect herbivory on leaves of the English oak (Quercus robur) trees. We chose the English oak as a model species as it is one of the most common and most emblematic forest trees in Europe, with a geographic range spanning more than 19 degrees of latitude. It is also widespread in both rural, suburban and urban environments. We exposed dummy plasticine caterpillars in trees to estimate predation rates. We additionally estimated leaf insect herbivory as the percentage of leaf area removed or damaged by insect herbivores. The Oak bodyguards project involved professional scientists and school children throughout Europe. We designed a simple protocol that was applied by both partner types. The protocol was written by scientists in collaboration with science instructors and communication officers. It was available in five languages: French, English, German, Spanish and Portuguese. The present document is the English version of the protocol. It describes how to estimate insect herbivory on oak leaves as well as predation rate on artificial prey made with green modelling clay. | BIOGECO - INRA - Univ. Bordeaux - 33610 Cestas - France, BIOGECO - INRA - Univ. Bordeaux - 33610 Cestas - France, Swiss Federal Institute for Forest - Snow and Landscape Research WSL, Forest Entomology - Swiss Federal Research Institute WSL Birmensdorf Switzerland | 1 | 2019 | Bastien Castagneyrol, Elena Valdés-Correcher, Michèle Kaennel Dobbertin, Martin Gossner 2019. Predation assessment on fake caterpillars and leaf sampling: Protocol for partner schools. protocols.io dx.doi.org/10.17504/protocols.io.42pgydn | 2021-03-29 03:11:02 | ||||
|
Phenzine Oxidizer Enrichment and Isolation Resource Report Resource Website |
Lev Tsypin, Yinon Bar-On, Scott Saunders, Jared R Leadbetter, Dianne K Newman | 10.17504/protocols.io.bh4tj8wn | This is a protocol for the enrichment of phenazine-1-carboxylic acid oxidizing microbes from a soil sample. | California Institute of Technology, Weizmann Institute of Science, California Institute of Technology, California Institute of Technology, California Institute of Technology | 1 | 2020 | Lev Tsypin, Yinon Bar-On, Scott Saunders, Jared R Leadbetter, Dianne K Newman 2020. Phenzine Oxidizer Enrichment and Isolation. protocols.io dx.doi.org/10.17504/protocols.io.bh4tj8wn | 2021-03-29 03:11:04 |
Can't find your Protocol?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific protocol and you know the DOI of the protocol already, it's easier to enter a DOI to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your protocol in the search results, please help us by adding it into the system — it's easy. Create and publish your protocols at Protocols.io.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
