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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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Flex-T™ Fixed Peptide Tetramer Preparation and Flow Cytometry Staining Protocol Resource Report Resource Website |
Sam Li | BioLegend | BioLegend | https://www.biolegend.com/en-us/protocols/flex-t-fixed-peptide-tetramer-preparation-and-flow-cytometry-staining-protocol | 1 | 2020 | Sam Li 2020. Flex-T™ Fixed Peptide Tetramer Preparation and Flow Cytometry Staining Protocol. protocols.io https:// | 2021-04-15 09:16:27 | ||||
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MojoSort™ Mouse anti-APC Nanobeads Column Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.699hh96 | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads than with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-mouse-anti-apc-nanobeads-column-protocol/4759/ | 1 | 2019 | Sam Li 2019. MojoSort™ Mouse anti-APC Nanobeads Column Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.699hh96 | 2021-04-15 09:16:16 | ||
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MojoSort™ Human NK Cell Isolation Kit Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7bihike | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody cocktail that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:2 to 1:10 for the antibody cocktail can be used. Dilutions ranging from 1:5 to 1:20 for the Streptavidin Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-nk-cell-isolation-kit-column-protocol/4622/ | 1 | 2019 | Sam Li 2019. MojoSort™ Human NK Cell Isolation Kit Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7bihike | 2021-04-15 09:16:33 | ||
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T Cell Activation with anti-CD3? Antibodies Resource Report Resource Website |
BioLegend, Inc. | DOI:10.17504/protocols.io.ezybf7w | BioLegend | This is a collection of BioLegend T Cell Activation with anti-CD3ε Antibodies protocols, for human and mouse. | , | http://www.biolegend.com/media_assets/support_protocol/BioLegend_T_cell_Activation_Protocol_012715.pdf | 1 | 2016 | BioLegend, Inc. 2016. T Cell Activation with anti-CD3? Antibodies. protocols.io https://dx.doi.org/10.17504/protocols.io.ezybf7w | 2021-04-15 09:16:19 | ||
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Western Blotting Protocol Resource Report Resource Website |
BioLegend, Inc. | DOI:10.17504/protocols.io.e2abgae | BioLegend | , | http://www.biolegend.com/media_assets/support_protocol/WB_Analysis_BetaAmyloid_12-04-14.pdf | 1 | 2016 | BioLegend, Inc. 2016. Western Blotting Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.e2abgae | 2021-04-15 09:16:20 | |||
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Propidium Iodide Cell Cycle Staining Protocol Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.tm8ek9w | BioLegend | BioLegend | https://www.biolegend.com/protocols/propidium-iodide-cell-cycle-staining-protocol/4303/ | 2 | 2018 | Kelsey Miller 2018. Propidium Iodide Cell Cycle Staining Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.tm8ek9w | 2021-04-15 09:16:40 | |||
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Immunocytochemistry Staining Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.968h9hw | BioLegend | BioLegend | https://www.biolegend.com/protocols/immunocytochemistry-staining-protocol/4275/ | 4 | 2019 | Sam Li 2019. Immunocytochemistry Staining Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.968h9hw | 2021-04-15 09:16:21 | |||
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MojoSort™ Isolation Kits Column Protocol - 3 Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7behije | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody cocktail that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:2 to 1:10 for the antibody cocktail can be used. Dilutions ranging from 1:5 to 1:20 for the Streptavidin Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-isolation-kits-column-protocol-3/4591/ | 1 | 2019 | Sam Li 2019. MojoSort™ Isolation Kits Column Protocol - 3. protocols.io https://dx.doi.org/10.17504/protocols.io.7behije | 2021-04-15 09:16:40 | ||
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True-Nuclear™ Transcription Factor Staining Protocols Resource Report Resource Website |
BioLegend, Inc. | DOI:10.17504/protocols.io.ezxbf7n | BioLegend | This is a collection of BioLegend protocols for True-Nuclear™ Transcription Factor Staining Protocols. Please make sure to refer to the correct protocol, depending on whether you are using the 96-Well, U-Bottom Plate or 5 mL tubes. | , | http://www.biolegend.com/media_assets/support_protocol/True-Nuclear_Trademark_Transcription_Factor_Staining_Protocol.pdf | 1 | 2016 | BioLegend, Inc. 2016. True-Nuclear™ Transcription Factor Staining Protocols. protocols.io https://dx.doi.org/10.17504/protocols.io.ezxbf7n | 2021-04-15 09:16:25 | ||
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MojoSort™ Streptavidin Nanobeads Protocol - Positive Selection Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7wghpbw | BioLegend | Product description and procedure summary:If your target cells are the labeled cells (the positive fraction), use the Streptavidin Nanobeads Protocol – Positive Selection. If your target cells are the unlabeled cells (negative fraction), use the Streptavidin Nanobeads Protocol - Negative Selection. Target cells are isolated by incubating your sample with a Biotin-conjugated antibody or antibody cocktail, followed by incubation with magnetic Streptavidin Nanobeads (Cat. No. 480015/480016). The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched cells are collected. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. Scale up volumes if using 14 mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-streptavidin-nanobeads-protocol-positive-selection/4748/ | 1 | 2019 | Sam Li 2019. MojoSort™ Streptavidin Nanobeads Protocol - Positive Selection. protocols.io https://dx.doi.org/10.17504/protocols.io.7wghpbw | 2021-04-15 09:16:25 | ||
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Chromatin Immunoprecipitation (ChIP) Assay Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.97gh9jw | BioLegend | BioLegend | https://www.biolegend.com/protocols/chromatin-immunoprecipitation-chip-assay-protocol/4306/ | 4 | 2019 | Sam Li 2019. Chromatin Immunoprecipitation (ChIP) Assay Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.97gh9jw | 2021-04-15 09:16:45 | |||
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Immunofluorescence Microscopy Protocol Resource Report Resource Website |
BioLegend, Inc. | DOI:10.17504/protocols.io.eyubfww | BioLegend | , | http://www.biolegend.com/media_assets/support_protocol/Immunofluorescence_Microscopy_Protocol_050514.pdf | 1 | 2016 | BioLegend, Inc. 2016. Immunofluorescence Microscopy Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.eyubfww | 2021-04-15 09:16:42 | |||
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MojoSort™ Mouse CD326 (Ep-CAM) Selection Kits Column Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7u4hnyw | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody than with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/en-us/protocols/mojosort-mouse-cd326-ep-cam-selection-kits-column-protocol | 1 | 2019 | Sam Li 2019. MojoSort™ Mouse CD326 (Ep-CAM) Selection Kits Column Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7u4hnyw | 2021-04-15 09:16:43 | ||
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MojoSort™ Mouse Neutrophil Isolation Kit Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7yuhpww | BioLegend | Product description and procedure summary:Target cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads (Cat. No. 480015/480016). The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched cells are collected. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. Scale up volumes if using 14mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | http://www.biolegend.com/media_assets/support_protocol/MojoSort_Mouse_Neutrophil_Isolation_Protocol_08072016.pdf | 2 | 2019 | Sam Li 2019. MojoSort™ Mouse Neutrophil Isolation Kit Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7yuhpww | 2021-04-15 09:16:26 | ||
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MojoSort™ Human NK Cell Isolation Kit Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7ythpwn | BioLegend | Product description and procedure summary:Target cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads (Cat. No. 480015/480016). The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched cells are collected. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. Scale up volumes if using 14mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-nk-cell-isolation-kit-protocol/4605/ | 1 | 2019 | Sam Li 2019. MojoSort™ Human NK Cell Isolation Kit Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7ythpwn | 2021-04-15 09:16:51 | ||
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Immunohistochemistry Protocol for Keratin Antibodies Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.95ph85n | BioLegend | BioLegend | https://www.biolegend.com/protocols/immunohistochemistry-protocol-for-keratin-antibodies/4287/ | 3 | 2019 | Sam Li 2019. Immunohistochemistry Protocol for Keratin Antibodies. protocols.io https://dx.doi.org/10.17504/protocols.io.95ph85n | 2021-04-15 09:16:26 | |||
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MojoSort™ Human CD56 Nanobeads Column Protocol Resource Report Resource Website |
Ken Lau | BioLegend | BioLegend | https://www.biolegend.com/en-us/protocols/mojosort-human-cd56-nanobeads-column-protocol | 2 | 2021 | Ken Lau 2021. MojoSort™ Human CD56 Nanobeads Column Protocol. protocols.io https:// | 2021-04-15 09:16:55 | ||||
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MojoSort™ Human CD4 Nanobeads Regular Protocol Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.e3hbgj6 | BioLegend | This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube from human peripheral blood mononuclear cells (PBMCs). If working with fewer cells keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. To maximize the purity of CD4+ T cells we recommend the depletion of monocytes, as human monocytes express CD4. To deplete monocytes from human PBMCs, we recommend the use of MojoSort™ Human CD14 Selection Kit (Cat. No. 480025/480026). Alternatively, you may consider the Human CD4 T Cell Selection Kit (Cat. No. 480038). After monocyte depletion proceed to isolate the CD4+ T cells. You can also use other methods to deplete monocytes. If you wish to include monocytes in your CD4+ cell isolation, depletion is not required. | BioLegend | http://www.biolegend.com/media_assets/support_protocol/MojoSort_Human_CD4_Nanobeads_Protocol_V02_10192015.pdf | 1 | 2016 | Kelsey Miller 2016. MojoSort™ Human CD4 Nanobeads Regular Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.e3hbgj6 | 2021-04-15 09:16:29 | ||
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MojoSort™ Human CD14 Nanobeads Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7x4hpqw | BioLegend | Product description and procedure summary:The cells targeted by the Nanobeads are either selected or depleted by incubating your sample with the directly conjugated magnetic particles. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. Scale up volumes if using 14 mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-cd14-nanobeads-protocol/4685/ | 2 | 2019 | Sam Li 2019. MojoSort™ Human CD14 Nanobeads Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7x4hpqw | 2021-04-15 09:16:56 | ||
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ELISPOT Protocol Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.e29bgh6 | BioLegend | BioLegend | http://www.biolegend.com/media_assets/support_protocol/BioLegend_ELISPOT_protocol_111511.pdf | 1 | 2016 | Kelsey Miller 2016. ELISPOT Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.e29bgh6 | 2021-04-15 09:16:57 |
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