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| Name | Authors | DOI | Group |
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Protocol for nuclei isolation from fresh and frozen tissues for parallel snRNA-Seq and snATAC-Seq on 10x ChromiumTM platform using the same nuclei preparation (UPDATED version) Resource Report Resource Website |
Luciano Martelotto | DOI:10.17504/protocols.io.bdeni3de | Human Cell Atlas Method Development Community | This protocol is an adaptation and extension of the 'Frankenstein' protocol – originally developed for nuclei isolation from fresh and frozen tissue for snRNA-Seq – in order to perform snATAC-Seq on the same nuclei prep. It has been successfully applied to fresh, snap/flash and cryopreserved frozen cell lines as well as to tissue derived from solid tumours and other tissues such as pancreas adenocarcinoma (PDAC), breast cancers, pheochromocytomas/paragangliomas, normal paraganglia, brain organoids, PDAC organoids, ovary, fallopian tube, mouse brain and sperm using the Chromium Platform (10x Genomics). | Harvard Medical School | 4 | 2020 | Luciano Martelotto 2020. Protocol for nuclei isolation from fresh and frozen tissues for parallel snRNA-Seq and snATAC-Seq on 10x ChromiumTM platform using the same nuclei preparation (UPDATED version). protocols.io https://dx.doi.org/10.17504/protocols.io.bdeni3de | 2021-04-15 09:15:33 | |||
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UNC Chapel Hill Zebrafish Aquaculture Core (ZAC) Environmental Summary Resource Report Resource Website |
Michelle Altemara | DOI:10.17504/protocols.io.3chgit6 | Zebrafish (Danio rerio) are a popular animal model used in a variety of research areas. As with all animal models, husbandry conditions, including environmental parameters, nutrition, and exposure to pathogens can affect research results. Additionly, zebrafish are tolerant of a wide range of environmental parameters, which has led to wide variation in husbandry practices across facilities around the world. The lack of standard conditions across zebrafish laboratories may play a role in irreproducible experiments. Therefore, a summary of these conditions for the year 2017 have been compiled for the Zebrafish Aquaculture Core (ZAC) facility at the University of North Carolina (UNC) Chapel Hill with the aim to have this data included in research articles published using our fish. As more institutions publish husbandry protocols, standard husbandry practices may emerge. | University of North Carolina at Chapel Hill | 2 | 2019 | Michelle Altemara 2019. UNC Chapel Hill Zebrafish Aquaculture Core (ZAC) Environmental Summary. protocols.io https://dx.doi.org/10.17504/protocols.io.3chgit6 | 2021-04-15 09:15:33 | ||||
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Generation of phage lysates of Brucella using Brucellaphage Resource Report Resource Website |
Hari Mohan Saxena, Sugandha Raj | DOI:10.17504/protocols.io.matc2en | A protocol for preparing phage lysate of Brucella organisms employing a lytic brucellaphage is given here. | Saxena HM, Raj S (2018) A novel immunotherapy of Brucellosis in cows monitored non invasively through a specific biomarker. PLoS Negl Trop Dis 12(4): e0006393. doi: 10.1371/journal.pntd.0006393 | Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India 141004., Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India 141004. | https://doi.org/10.1371/journal.pntd.0006393 | 1 | 2017 | Hari Mohan Saxena, Sugandha Raj 2017. Generation of phage lysates of Brucella using Brucellaphage. protocols.io https://dx.doi.org/10.17504/protocols.io.matc2en | 2021-04-15 09:15:33 | ||
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Human platelet isolation Resource Report Resource Website |
Allen Bennett | DOI:10.17504/protocols.io.pymdpu6 | Platelets are the primary cellular mediator of thrombosis. This protocol allows for the preparation of washed human platelets. These platelets can then be stimulated or cultured for various purposes. | University of Rochester | 1 | 2018 | Allen Bennett 2018. Human platelet isolation. protocols.io https://dx.doi.org/10.17504/protocols.io.pymdpu6 | 2021-04-15 09:15:50 | ||||
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UCHANGE structural neuroimaging pipeline Resource Report Resource Website |
Kirstie Whitaker | DOI:10.17504/protocols.io.eu5bey6 | Brain Mapping Unit, Department of Psychiatry, University of Cambridge | 1 | 2017 | Kirstie Whitaker 2017. UCHANGE structural neuroimaging pipeline. protocols.io https://dx.doi.org/10.17504/protocols.io.eu5bey6 | 2021-04-15 09:15:32 | |||||
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The production of monocytes and macrophages from human iPSCs Resource Report Resource Website |
Katherine Santostefano, Jared Taylor, Naohiro Terada, Mark Wallet | DOI:10.17504/protocols.io.m8kc9uw | Human Islet Research Network, Consortium on Modeling Autoimmune Interactions | This protocol is a modification of a previously published method by Wilgenburg et al (PLoS One, 2013) to obtain monocytes and macrophages from induced Pluripotent Stem Cells (iPSCs) lines. | Department of Pathology, Immunology & Laboratory Medicine, University of Florida, Gainesville, FL, USA, Department of Pathology, Immunology & Laboratory Medicine, University of Florida, Gainesville, FL, USA, Department of Pathology, Immunology & Laboratory Medicine, University of Florida, Gainesville, FL, USA, Department of Pathology, Immunology & Laboratory Medicine, University of Florida, Gainesville, FL, USA | 1 | 2018 | Katherine Santostefano, Jared Taylor, Naohiro Terada, Mark Wallet 2018. The production of monocytes and macrophages from human iPSCs. protocols.io https://dx.doi.org/10.17504/protocols.io.m8kc9uw | 2021-04-15 09:15:32 | |||
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Protocols for "The female urinary microbiota in relation to the reproductive tract microbiota." Resource Report Resource Website |
Chen Chen, Lilan Hao, Weixia Wei, Fei Li, Liju Song, Xiaowei Zhang, Juanjuan Dai, Zhuye Jie, Jiandong Li, Xiaolei Song, Zirong Wang, Zhe Zhang, Liping Zeng, Hui Du, Huiru Tang, Tao Zhang, Huanming Yang, Jian Wang, Susanne Brix, Karsten Kristiansen, Xun Xu, Ruifang Wu, Huijue Jia | DOI:10.17504/protocols.io.bp3wmqpe | GigaScience Press, BGI | Human urine is traditionally considered to be sterile, and whether the urine harbours distinct microbial communities has been a matter of debate. Potential links between female urine and reproductive tract microbial communities is currently not clear. Here, we collected urine samples from 147 Chinese women of reproductive age and explored the nature of colonization by 16S rRNA gene amplicon sequencing, quantitative real-time PCR, and live bacteria culture. To demonstrate the utility of this approach, the intra-individual Spearman's correlation was used to explore the relationship between urine and multiple sites of the reproductive tract. PERMANOVA was also performed to explore potential correlations between the lifestyle and various clinical factors and urinary bacterial communities. Our data demonstrated distinct bacterial communities in urine, indicative of a non-sterile environment. Streptococcus-dominated, Lactobacillus-dominated, and diverse type were the three most common urinary bacterial community types in the cohort. Detailed comparison of the urinary microbiota with multiple sites of the female reproductive tract microbiota demonstrated that the urinary microbiota were more similar to the microbiota in the cervix and uterine cavity than to those of the vagina in the same women. Our data demonstrate the potential connectivity among microbiota in the female urogenital system and provide insight and resources for exploring diseases of the urethra and genital tract. | BGI-Shenzhen, Shenzhen 518083, China., BGI-Shenzhen, Shenzhen 518083, China., Peking University Shenzhen Hospital, Shenzhen 518036, China., BGI-Shenzhen, Shenzhen 518083, China., BGI-Shenzhen, Shenzhen 518083, China., BGI-Shenzhen, Shenzhen 518083, China., Peking University Shenzhen Hospital, Shenzhen 518036, China., BGI-Shenzhen, Shenzhen 518083, China., BGI-Shenzhen, Shenzhen 518083, China., BGI-Shenzhen, Shenzhen 518083, China., BGI-Shenzhen, Shenzhen 518083, China., BGI-Shenzhen, Shenzhen 518083, China., Peking University Shenzhen Hospital, Shenzhen 518036, China., Peking University Shenzhen Hospital, Shenzhen 518036, China., Peking University Shenzhen Hospital, Shenzhen 518036, China., BGI-Shenzhen, Shenzhen 518083, China., BGI-Shenzhen, Shenzhen 518083, China., BGI-Shenzhen, Shenzhen 518083, China., Department of Biotechnology and Biomedicine, Technical University of Denmark, Soltofts Plads, Building 221, 2800 Kgs. Lyngby, Denmark., BGI-Shenzhen, Shenzhen 518083, China., BGI-Shenzhen, Shenzhen 518083, China., Peking University Shenzhen Hospital, Shenzhen 518036, China., BGI-Shenzhen, Shenzhen 518083, China. | 4 | 2020 | Chen Chen, Lilan Hao, Weixia Wei, Fei Li, Liju Song, Xiaowei Zhang, Juanjuan Dai, Zhuye Jie, Jiandong Li, Xiaolei Song, Zirong Wang, Zhe Zhang, Liping Zeng, Hui Du, Huiru Tang, Tao Zhang, Huanming Yang, Jian Wang, Susanne Brix, Karsten Kristiansen, Xun Xu, Ruifang Wu, Huijue Jia 2020. Protocols for "The female urinary microbiota in relation to the reproductive tract microbiota.". protocols.io https://dx.doi.org/10.17504/protocols.io.bp3wmqpe | 2021-04-15 09:15:32 | |||
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Bleach synchronisation of C. elegans Resource Report Resource Website |
Ida Barlow | DOI:10.17504/protocols.io.2bzgap6 | Behavioural Genomics | This protocol is for obtaining synchronised populations of C. elegans. Eggs are harvested from gravid hermaphrodites and allow to grow to L1 arrest for 12-120 hours (0.5-5days). L1 larvae are then fed and left to develop to the appropriate developmental stage | Imperial College London | 1 | 2019 | Ida Barlow 2019. Bleach synchronisation of C. elegans. protocols.io https://dx.doi.org/10.17504/protocols.io.2bzgap6 | 2021-04-15 09:15:33 | |||
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Diagnosis and treatment of plasmablastic lymphoma, plasmablastic myeloma and plasmablastic neoplasm: protocol for a scoping review Resource Report Resource Website |
Hirotaka Mori, Masahiko Fukatsu, Hiroshi Ohkawara, Yuka Oka, Yuki Kataoka, Shunsuke Taito, Takayuki Ikezoe | DOI:10.17504/protocols.io.beyzjfx6 | IntroductionThe differential diagnosis of plasmablastic lymphoma (PBL) form plasmablastic myeloma (PBM) is sometime very difficult. In such cases, the diagnosis of plasmablastic neoplasm (PBN) is acceptable. But clinicians face the question whether chemotherapies for PBL, PBM or others are effective for PBN and the question increases the diagnosis dilemma. Although evidence on the differential diagnosis and treatment of those three entities is compiling, there is no scopingreview of different diagnosis and treatment for PBL, PBM, and PBN to answer those questions. The aim of this study is to develop an a priori protocol to undertake a scoping review of the available evidence on the reproducibility of differential diagnosis and the treatment of PBL, PBM, and PBN. Methods and analysisFor the conducting the scoping review protocol, we will use an scoping review methodology established in the Joanna Briggs Institute manual. This methodology has a five-stage approach: (1) identify the research question; (2) identify relevant studies; (3) select studies; (4) chart the data and (5) collating, summarize and report the results. We also will use the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews to assess the results.Ethics and disseminationSince this is a review of the literature, ethics approval is not indicated. We will disseminate the findings from this study in publications in peer-reviewed journals. | Department of Hematology, Fukushima Medical University, Fukushima 960-1295, Japan, Department of Hematology, Fukushima Medical University, Fukushima 960-1295, Japan, Department of Hematology, Fukushima Medical University, Fukushima 960-1295, Japan, Department of Diagnostic Pathology, Fukushima Medical University, Fukushima, 960-1295, Japan, Hospital Care Research Unit, Hyogo Prefectural Amagasaki General Medical Center, Higashinaniwa-cho 2-17-77, Amagasaki 660-8550 JAPAN. Department of Healthcare Epidemiology, Graduate School of Medicine and Public Health, Kyoto University, Yoshida Konoe-cho, Sakyo-ku, Kyoto 606-8501 Japan. Systematic Review Workshop Peer Support Group (SRWS-PSG), Japan. Department of Respiratory Medicine, Hyogo Prefectural Amagasaki General Medical Center, Higashinaniwa-cho 2-17-77, Amagasaki 660-8550 Japan., Systematic Review Workshop Peer Support Group (SRWS-PSG), Japan. Division of Rehabilitation, Department of Clinical Practice and Support, Hiroshima University Hospital, Kasumi 1-2-3, Minami-ku, Hiroshima, 734-8551 Japan, Department of Hematology, Fukushima Medical University, Fukushima, 960-1295, Japan | 1 | 2020 | Hirotaka Mori, Masahiko Fukatsu, Hiroshi Ohkawara, Yuka Oka, Yuki Kataoka, Shunsuke Taito, Takayuki Ikezoe 2020. Diagnosis and treatment of plasmablastic lymphoma, plasmablastic myeloma and plasmablastic neoplasm: protocol for a scoping review. protocols.io https://dx.doi.org/10.17504/protocols.io.beyzjfx6 | 2021-04-15 09:15:50 | ||||
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Using rhythmic auditory stimuli to modulate resilience to perturbations during walking in healthy young adults Resource Report Resource Website |
Deepak Ravi | DOI:10.17504/protocols.io.bmu3k6yn | Institute for Biomechanics, ETH Zürich, Zürich, Switzerland | 2 | 2020 | Deepak Ravi 2020. Using rhythmic auditory stimuli to modulate resilience to perturbations during walking in healthy young adults. protocols.io https://dx.doi.org/10.17504/protocols.io.bmu3k6yn | 2021-04-15 09:15:33 | |||||
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Identifying Viral Sequences Using VirSorter (Cyverse) Resource Report Resource Website |
Benjamin Bolduc | DOI:10.17504/protocols.io.ev2be8e | Sullivan Lab | Identifying putative viral sequences from SPAdes-assembled data from the Ocean Sampling Day (2014) metagenomic datasets using VirSorter. | Bolduc B, Youens-Clark K, Roux S, Hurwitz BL, Sullivan MB, iVirus: facilitating new insights in viral ecology with software and community data sets imbedded in a cyberinfrastructure. The ISME Journal 11(1). doi: 10.1038/ismej.2016.89 | The Ohio State University | https://doi.org/ismej201689 | 1 | 2016 | Benjamin Bolduc 2016. Identifying Viral Sequences Using VirSorter (Cyverse). protocols.io https://dx.doi.org/10.17504/protocols.io.ev2be8e | 2021-04-15 09:15:33 | |
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Transposase injection mix protocol Resource Report Resource Website |
Ben Matthews | DOI:10.17504/protocols.io.4a6gshe | This protocol details the steps necessary to generate injection mixes (comprised of plasmid DNA for integration and transposase mRNA) for transposon-mediated integration into Aedes aegypti and other insects. It has been empirically tested in Ae. aegypti embryos with piggyBac transposase. In our hands, using mRNA as a source of transposase instead of a helper plasmid increases transformation rates substantially. | University of British Columbia | 1 | 2019 | Ben Matthews 2019. Transposase injection mix protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.4a6gshe | 2021-04-15 09:15:32 | ||||
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ELISA for quantification of IL-11 in human serum. Resource Report Resource Website |
Angel Justiz-Vaillant | DOI:10.17504/protocols.io.bj3akqie | Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Bone marrow stromal cells and fibroblasts produce IL-11. The IL-11 principal targets are hemopoietic progenitors and osteoclasts. The IL-11 primary effects include osteoclast formation, colony stimulating factor, raised platelet count in vivo, and inhibition of pro-inflammatory cytokine production. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for quantification of IL-11 in human serum.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj3akqie | 2021-04-15 09:15:50 | ||||
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How to expose existing R code as a web service Resource Report Resource Website |
Sonia García-Ruiz | Ryten Laboratory | University College London, University of London | https://rytenlab.com/swagger/index.html | 2 | 2021 | Sonia García-Ruiz 2021. How to expose existing R code as a web service. protocols.io https:// | 2021-04-15 09:15:32 | ||||
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Two-step protocol: Preparation and extrusion of phospholipid liposomes Resource Report Resource Website |
James R. Collins, Krista Longnecker, Helen F. Fredricks, Benjamin A. S. Van Mooy | DOI:10.17504/protocols.io.haub2ew | Van Mooy Lab @ WHOI | The protocols in this collection were original created by Krista Longnecker and Jamie Collins for creating lipsomes to be used in lipid photo-oxidation experiments. The results of these experiments are detailed in Chapter 4 of:Collins, J. R. 2017. The remineralization of marine organic matter by diverse biological and abiotic processes. Ph.D. thesis. Cambridge, Massachusetts: Massachusetts Institute of Technology, 300 pp; doi:10.1575/1912/8721A manuscript of the chapter is forthcoming. | Woods Hole Oceanographic Institution, Woods Hole Oceanographic Institution, Woods Hole Oceanographic Institution, Woods Hole Oceanographic Institution | http://dx.doi.org/10.1575/1912/8721 | 2 | 2017 | James R. Collins, Krista Longnecker, Helen F. Fredricks, Benjamin A. S. Van Mooy 2017. Two-step protocol: Preparation and extrusion of phospholipid liposomes. protocols.io https://dx.doi.org/10.17504/protocols.io.haub2ew | 2021-04-15 09:15:32 | ||
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Organization for Lab Data using Bash Resource Report Resource Website |
Courtney Comrie | DOI:10.17504/protocols.io.7h4hj8w | Organization is important in the IPA and MSBIL | University of Arizona | 1 | 2019 | Courtney Comrie 2019. Organization for Lab Data using Bash. protocols.io https://dx.doi.org/10.17504/protocols.io.7h4hj8w | 2021-04-15 09:15:32 | ||||
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The Association between Workplace Violence and Physicians' and Nurses' Job Satisfaction in Macau Resource Report Resource Website |
Teris Cheung, Paul H. Lee, Paul S.F. Yip | DOI:10.17504/protocols.io.tcyeixw | AbstractBackgroundThis paper describes the association between workplace violence and job satisfaction among physicians and nurses in Macau. It further examines the risk factors for intrinsic and extrinsic job satisfaction among these professionals.MethodsConvenience sampling was sourced from six health centers supervised by the Macau Health Bureau, namely, the Coloane Health Center, Coloane Shek Pai Wan Temporary Health Center, Taipa Elderly Macau University of Science and Technology Hospital, Macau Sino-Portuguese Nurses Association, Macau Association of Medical Volunteers and the Macau Surgical Association. Design: This study uses a cross-sectional self-administrative survey. Instruments: The study used case studies research instruments for workplace violence in the health sector by country (from the ILO, ICN, WHO, PSI) and the Minnesota Satisfaction Questionnaire and Perceived Stress Scale. The data collection period spanned from August to December, 2014. Data analysis: Multiple logistic regression examines levels of intrinsic and extrinsic satisfaction in physicians and nurses and significant correlates affecting their job satisfaction.ResultsA total of 720 (14.9% physicians) participants were recruited. Worry about WPV, on-call duty and shift work, experience of bullying and verbal abuse and employment sector emerged as significant correlates affecting the intrinsic and extrinsic job satisfaction of physicians and nurses. Working on the frontline, being aged 30 to 39, coming from an ethnic minority, and perceived stress were significant correlates affecting nurses’ job satisfaction.ConclusionsWPV remains a significant concern in healthcare settings in Macau. Healthcare professionals should deploy protective strategies to combat the negative psychological effects of WPV. Stakeholders should legally enforce a zero-tolerance policy towards WPV within healthcare workplaces. The perpetrators of WPV should be told that violence will be deemed a violation of human rights and not tolerated, not least on account of how WPV is detrimental to healthcare professionals’ mental wellbeing, risking irreversible physical and psychological harm for its victims.Keywords: Workplace Violence, Physicians, Nurses, Job satisfaction, Occupational health, Macau | Cheung T, Lee PH, Yip PSF (2018) The association between workplace violence and physicians’ and nurses’ job satisfaction in Macau. PLoS ONE 13(12): e0207577. doi: 10.1371/journal.pone.0207577 | School of Nursing, Hong Kong Polytechnic University, Hong Kong, School of Nursing, Hong Kong Polytechnic University, Hong Kong, Centre for Suicide Research and Prevention, University of Hong Kong, Hong Kong | https://doi.org/10.1371/journal.pone.0207577 | 1 | 2018 | Teris Cheung, Paul H. Lee, Paul S.F. Yip 2018. The Association between Workplace Violence and Physicians' and Nurses' Job Satisfaction in Macau. protocols.io https://dx.doi.org/10.17504/protocols.io.tcyeixw | 2021-04-15 09:15:32 | ||
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Phylogenetic analyses of Ophiothrix (Echinodermata: Ophiuroidea) Resource Report Resource Website |
Renata Alitto, Michela Borges, Letícia Dias de Oliveira, Karin Regina Seger, Luciana Bolsoni Lourenço | DOI:10.17504/protocols.io.wimfcc6 | Using 16S and COI fragmens in Bayesian Inference, Maximum Parsimony, and Maximum Likelihood. | Alitto RAdS, Amaral ACZ, Oliveira LDd, Serrano H, Seger KR, Guilherme PDB, Domenico MD, Christensen AB, Lourenço LB, Tavares M, Borges M (2019) Atlantic West Ophiothrix spp. in the scope of integrative taxonomy: Confirming the existence of Ophiothrix trindadensis Tommasi, 1970. PLoS ONE 14(1): e0210331. doi: 10.1371/journal.pone.0210331 | UNICAMP, UNICAMP, UNICAMP, UNICAMP, UNICAMP | https://doi.org/10.1371/journal.pone.0210331 | 3 | 2019 | Renata Alitto, Michela Borges, Letícia Dias de Oliveira, Karin Regina Seger, Luciana Bolsoni Lourenço 2019. Phylogenetic analyses of Ophiothrix (Echinodermata: Ophiuroidea). protocols.io https://dx.doi.org/10.17504/protocols.io.wimfcc6 | 2021-04-15 09:15:32 | ||
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Extracting total RNA from Caenorhabditis elegans using phase-lock gel separation tubes Resource Report Resource Website |
Cristian Riccio | DOI:10.17504/protocols.io.3pzgmp6 | This protocol describes how to extract total RNA from C. elegans worms starting with 3300 worms on a 90mm NGM plate | Cancer Research UK / Wellcome Gurdon Institute | 1 | 2019 | Cristian Riccio 2019. Extracting total RNA from Caenorhabditis elegans using phase-lock gel separation tubes. protocols.io https://dx.doi.org/10.17504/protocols.io.3pzgmp6 | 2021-04-15 09:15:33 | ||||
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Extracorporeal membrane oxygenation meta-analysis of time-to-event data in respiratory failure in adults Resource Report Resource Website |
Hyunsuk Frank Roh, Chang-Guk Kim, Soon-Ho Chon, Jung Mogg Kim | DOI:10.17504/protocols.io.bb7nirme | Purpose:Time-to-event data of hazard ratios were used to generate a forest plot of mortality on extracorporeal membrane oxygenation (ECMO) in respiratory failure in adults.Materials and Methods:A systemic search was conducted in PubMed from 1975 to 2018. Among 4,121 articles, a total of 5 clinical reports comprising 1,014patients (333 of the patients who underwent ECMO and 681 of the patients who did not undergo ECMO) met the inclusion criteria.Results:The pooled hazard ratio of 0.64 (95% confidence interval (CI): 0.54, 0.76) suggests that the ECMO group comprising both veno-arterial (VA) type and a majority of veno-venous (VV) type was significantly associated with a reduction in mortality compared with the non-ECMO group in respiratory failure. The results of VV-ECMO alone also showed significantly improved patient survival of respiratory failure with the hazard ratio of 0.67 (0.56, 0.81).Conclusions:The results of the ECMO group with VV and VA types, or the VV type alone revealed a reduction in mortality for patients with respiratory failure based on time-to-event data.Future investigation of the efficacy of VA-ECMO alone in respiratory failure may be more enlightening. | Emergency Medicine and Echocardiography Laboratory, Dongjak Kyunghee Hospital, Seoul, Korea. nGene Hemodynamic Research Center., Department of Medicine, Hanyang University College of Medicine, Seoul, Korea., Department of Thoracic and Cardiovascular Surgery, Hando General Hospital, Ansan City, Gyunggi, Korea., Department of Microbiology and Biomedical Science, Hanyang University College of Medicine and Graduate School of Biomedical Science and Engineering, Seoul, Korea. | 1 | 2020 | Hyunsuk Frank Roh, Chang-Guk Kim, Soon-Ho Chon, Jung Mogg Kim 2020. Extracorporeal membrane oxygenation meta-analysis of time-to-event data in respiratory failure in adults. protocols.io https://dx.doi.org/10.17504/protocols.io.bb7nirme | 2021-04-15 09:15:34 |
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