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Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the correct protocol if you are using the C3019H cells. If you are using the C3019I cells, please refer to this protocol.
Proper citation: New England Biolabs 2017. High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (C3019H). protocols.io https://dx.doi.org/10.17504/protocols.io.jxycppw Copy
Authors: Isabel Gautreau
Group: New England Biolabs (NEB)
Proper citation: Isabel Gautreau 2020. NEBNext Ultra End Prep Mixture E7442. protocols.io https://dx.doi.org/10.17504/protocols.io.k6yczfw Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB), Coronavirus Method Development Community
Summary: This protocol details methods for the NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®), NEB #E7658S/L 24/96 reactions.
Proper citation: New England Biolabs 2021. NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®) E7658. protocols.io https://dx.doi.org/10.17504/protocols.io.bsy5nfy6 Copy
Authors: Isabel Gautreau
Group: New England Biolabs (NEB)
Summary: This protocol is a group of the following 5 methods from the NEB website:1. NEBNext End Prep 2. Adaptor Ligation3. Size Selection of Adaptor-ligated DNA4. PCR Amplification5. Cleanup of PCR Amplification
Proper citation: Isabel Gautreau 2015. NEBNext® Ultra™ DNA Library Prep Protocol for Illumina® With Size Selection (E7370). protocols.io https://dx.doi.org/10.17504/protocols.io.cg5ty5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is a generic PNGase F protocol with denaturing reaction conditions. It is appropriate for both P0704 and P0708.
Proper citation: New England Biolabs 2015. PNGase F Protocol, Denaturing Conditions. protocols.io https://dx.doi.org/10.17504/protocols.io.cqfvtm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the correct protocol if you are using the C3019I cells. If you are using the C3019H cells, please refer to this protocol.
Proper citation: New England Biolabs 2015. High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (C3019I). protocols.io https://dx.doi.org/10.17504/protocols.io.cf5tq5 Copy
Authors: NEB
Group: New England Biolabs (NEB)
Proper citation: NEB 2015. E2050 Modified Nucl Mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.cv8w9v Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Quick CIP is a heat-labile version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source.Rapid and irreversible heat inactivation eliminates unwanted activityImproved storage stability versus native enzymeFaster reaction setup (no supplemental additives like zinc required) and shorter incubation timeFlexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)Less enzyme required (high specific activity), resulting in a lower cost per reactionNo need for multiple phosphatases (Quick CIP removes 5′- and 3′- phosphates from DNA, RNA and dNTPs )Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysisRecombinant for purity, consistency and value
Proper citation: New England Biolabs 2020. Protocol for Dephosphorylation of 5′ ends of DNA using Quick CIP (NEB #M0525). protocols.io https://dx.doi.org/10.17504/protocols.io.7r2hm8e Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This transformation protocol is for the C2527I cells. (For the C2527H protocol, see here.)
Proper citation: New England Biolabs 2015. Transformation Protocol for BL21(DE3) Competent Cells (C2527I). protocols.io https://dx.doi.org/10.17504/protocols.io.crjv4m Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. M0371 no RE mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.crmv45 Copy
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