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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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30% Sucrose for Cryoprotection of brains after Perfusions Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bg5ujy6w | BICCN, Allen Institute for Brain Science | This protocol is used to prepare 30% Sucrose Solution in 0.1M phosphate buffer. 30% Sucrose in 0.1M phosphate buffer is used for the cryoprotection of mice brains post perfusion.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 2 | 2020 | Allen Institute for Brain Science 2020. 30% Sucrose for Cryoprotection of brains after Perfusions. protocols.io https://dx.doi.org/10.17504/protocols.io.bg5ujy6w | 2021-04-15 09:16:34 | |||
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TissueCyte Specimen Embedding Acrylamide Coembedding Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bf65jrg6 | BICCN, Allen Institute for Brain Science | This protocol describes the preparation of a fixed mouse brain specimen bonded to an agarose block for blockface imaging and sectioning using the TissueCyte 1000 system.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 4 | 2020 | Allen Institute for Brain Science 2020. TissueCyte Specimen Embedding Acrylamide Coembedding. protocols.io https://dx.doi.org/10.17504/protocols.io.bf65jrg6 | 2021-04-15 09:16:31 | |||
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BD FACS Aria II Start-up and Calibration Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bamdic26 | BICCN, Allen Institute for Brain Science | This protocol provides step by step instructions for performing single-cell sorting by using BD FACSAria II machine and BD FACSDiva software in preparation for single-cell sorting. | Allen Institute | 1 | 2019 | Allen Institute for Brain Science 2019. BD FACS Aria II Start-up and Calibration. protocols.io https://dx.doi.org/10.17504/protocols.io.bamdic26 | 2021-04-15 09:16:57 | |||
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TissueCyte Troubleshooting Guide Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bdpsi5ne | BICCN, Allen Institute for Brain Science | This protocol outlines strategies to solve common problems that occur when setting up the TissueCyte 1000 system for normal operation.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 2 | 2020 | Allen Institute for Brain Science 2020. TissueCyte Troubleshooting Guide. protocols.io https://dx.doi.org/10.17504/protocols.io.bdpsi5ne | 2021-04-15 09:16:55 | |||
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Core Protocol for Serial Imaging of Fluorescently-Labeled Mouse Brain on the TissueCyte 1000 System Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.be2ejgbe | BICCN, Allen Institute for Brain Science | This protocol describes the instructions for setup and scanning of fluorescently-labeled tissue sections mounted in agarose and attached to a slide using the TissueCyte 1000 block face 2-photon system.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 4 | 2020 | Allen Institute for Brain Science 2020. Core Protocol for Serial Imaging of Fluorescently-Labeled Mouse Brain on the TissueCyte 1000 System. protocols.io https://dx.doi.org/10.17504/protocols.io.be2ejgbe | 2021-04-15 09:16:52 | |||
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Slice Preparation and Blockface Imaging for Electrophysiology - Mouse Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bcsdiwa6 | BICCN, Allen Institute for Brain Science | This protocol describes the procedure for transcardial perfusion of an adult mouse, followed by dissection, embedding, slicing, and blockface imaging of the mouse brain using either a Leica vibratome or a compresstome. The output tissue is suitable for use in electrophysiological recordings and/or live cell filling applications. | Allen Institute | 1 | 2020 | Allen Institute for Brain Science 2020. Slice Preparation and Blockface Imaging for Electrophysiology - Mouse. protocols.io https://dx.doi.org/10.17504/protocols.io.bcsdiwa6 | 2021-04-15 09:17:02 | |||
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Tissue Quality Evaluation for Brain Perfusion/Dissection Specimens Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.besmjec6 | BICCN, Allen Institute for Brain Science | This protocol provides guidance in the assessment of quality of tissues post perfusion and dissection and applies to all such procedures. Perfusion and dissection quality will be categorized on the following scale: perfect, good, fair, and poor. This SOP also presents written descriptions, and images, of specific category attributes. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 2 | 2020 | Allen Institute for Brain Science 2020. Tissue Quality Evaluation for Brain Perfusion/Dissection Specimens. protocols.io https://dx.doi.org/10.17504/protocols.io.besmjec6 | 2021-04-15 09:17:03 | |||
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Coverslipping: Use of the Leica CV5030 Coverslipper Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bg4xjyxn | BICCN, Allen Institute for Brain Science | This protocol describes the coverslipping of glass slides with the Leica CV5030.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 1 | 2020 | Allen Institute for Brain Science 2020. Coverslipping: Use of the Leica CV5030 Coverslipper. protocols.io https://dx.doi.org/10.17504/protocols.io.bg4xjyxn | 2021-04-15 09:17:02 | |||
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TissueCyte Image QC Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bdpri5m6 | BICCN, Allen Institute for Brain Science | This protocol describes the QC step that occurs after the week of TissueCyte runs, and prior to analysis.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 2 | 2020 | Allen Institute for Brain Science 2020. TissueCyte Image QC. protocols.io https://dx.doi.org/10.17504/protocols.io.bdpri5m6 | 2021-04-15 09:17:07 | |||
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Multi-Patch Clamp Recording Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bdpgi5jw | BICCN, Allen Institute for Brain Science | This protocol describes the process to obtain multiple electrophysiological recordings from postnatal mouse and/or human brain slices.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 2 | 2020 | Allen Institute for Brain Science 2020. Multi-Patch Clamp Recording. protocols.io https://dx.doi.org/10.17504/protocols.io.bdpgi5jw | 2021-04-15 09:17:06 |
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