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Authors: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
Group: University of the West Indies, [email protected]

Proper citation: Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for measurement of serum macrophage migration inhibitory factor (MIF). . protocols.io https://dx.doi.org/10.17504/protocols.io.bj7ekrje Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukin-10 is a pleiotropic cytokine playing a critical role as a regulator of myeloid and lymphoid cell function. Due to the ability of IL-10 to blocking cytokine synthesis and many accessory cell functions of antigen-presenting cells such as macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. IL-10 also participates in regulating proliferation and differentiation of B-lymphocytes, mast cells and thymocytes [1]. Reference1. Ouyang W, O'Garra A. IL-10 Family Cytokines IL-10 and IL-22: from Basic Science to Clinical Translation.Immunity. 2019;50(4):871-891. doi:10.1016/j.immuni.2019.03.020

Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-6 in human serum or plasma.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjy7kpzn Copy   

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Authors: Angel Justiz-Vaillant, Suzette Curtello
Group: University of the West Indies, [email protected]

Proper citation: Angel Justiz-Vaillant, Suzette Curtello 2020. Salmonella live attenuated vaccine preparation.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjpdkmi6 Copy   

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Authors: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
Group: University of the West Indies, [email protected]

Proper citation: Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of Granulocyte-colony stimulator factor (G-CSF) in human serum or plasma. protocols.io https://dx.doi.org/10.17504/protocols.io.bks2kwge Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Chemical synthesis facilitates the generation of peptides which are difficult to express in bacteria. The fragment 254-274 of the human immunodeficiency virus (HIV) is critical for infectivity of the virus and induction of antibody neutralization (Ho et al, 1988). Boudet et al, 1995 demonstrated that a synthetic peptide derived from the third variable domain of the HIV-1 gp-120 when used as immunogen was able to induce an antibody response to multiple (up to six) HIV strains. ReferenceHo DD, Kaplan JC, Rackauskas IE, Gurney ME. Second conserved domain of gp120 is important for HIV infectivity and antibody neutralization.Science. 1988;239(4843):1021-1023. doi:10.1126/science.2830667.Boudet F, Keller H, Kieny MP, Thèze J. Single peptide and anti-idiotype based immunizations can broaden the antibody response against the variable V3 domain of HIV-1 in mice.Mol Immunol. 1995;32(7):449-457. doi:10.1016/0161-5890(95)00007-2

Proper citation: Angel Justiz-Vaillant 2020. Conjugation of Keyhole limpet haemocynin to Peptide 254-274 of HIV gp-120 as immunogen.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjh7kj9n Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Protein LAG (SpLAG) is an immunoglobulin-binding protein that interacts with the Fc and Fab regions of many mammalian immunoglobulins. It is produced by a chemical coupling of individual proteins and them mixing it up to the appropriate protein ratio. SpLAG binds well to some avian immunoglobulin [1].References1.Vaillant AJ, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. (2013) Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 4: 175. doi:10.4172/2155-9872.1000175

Proper citation: Angel Justiz-Vaillant 2020. Direct ELISA for investigating the binding of chemically-made Protein-LAG  to immunoglobulins.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj4zkqx6 Copy   

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Authors: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
Group: University of the West Indies, [email protected]
Summary: Granulocyte macrophage-colony stimulating factor (GM-CSF) is a monomeric glycoprotein. It is a cytokine secreted by macrophages, T cells, natural killer cells, mast cells, endothelial cells and fibroblasts. It acts as a growth factor. [1] Reference1. Egea L, Hirata Y, Kagnoff MF. GM-CSF: a role in immune and inflammatory reactions in the intestine. Expert Rev Gastroenterol Hepatol. 2010 Dec;4(6):723-31. doi: 10.1586/egh.10.73. PMID: 21108592; PMCID: PMC3291482.

Proper citation: Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of granulocyte macrophage-colony stimulating factor (GM-CSF) in tissue culture supernatant, human serum or plasma.. protocols.io https://dx.doi.org/10.17504/protocols.io.bktykwpw Copy   

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Authors: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
Group: University of the West Indies, [email protected]

Proper citation: Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of human properdin in serum or plasma.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj7zkrp6 Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Phagocytes mainly make IL-36. It acts on T lymphocytes and NK cells regulating the IFN-γ synthesis. It stimulates the hematopoiesis and expression of both MHC class I and II molecules as well as intracellular adhesion molecules (ICAM)-1. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.

Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-36 in human serum.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj38kqrw Copy   

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Authors: Angel Justiz-Vaillant, Monica F. Smikle
Group: University of the West Indies, [email protected]
Summary: Referencesst protein LA (SpLA) is an immunoglobulin-binding protein that interacts with the Fab and Fc regions of many mammalian immunoglobulins [1].References1.Vaillant AJ, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. (2013) Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 4: 175. doi:10.4172/2155-9872.1000175

Proper citation: Angel Justiz-Vaillant, Monica F. Smikle 2020. Direct ELISA for investigating the binding of recombinant Protein-LA  to immunoglobulins.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjxukpnw Copy   

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Authors: Angel Justiz-Vaillant, Monica F. Smikle
Group: University of the West Indies, [email protected]

Proper citation: Angel Justiz-Vaillant, Monica F. Smikle 2020. Direct ELISA for investigating the binding of Protein-A to immunoglobulins. . protocols.io https://dx.doi.org/10.17504/protocols.io.bjxqkpmw Copy   

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Authors: Angel Justiz-Vaillant, Patrick E. Akpaka
Group: University of the West Indies, [email protected]
Summary: This test shows the ability of Ab-3 antibody to inhibit the growth of S. aureus in vitro. The amounts of samples tested were 18 samples from chicks fed with hyper-immune eggs and therefore have the Ab-3, and six samples from chicks fed non-hyperimmune eggs and defenetively do not have the Ab-3. Inhibition of the growth of the bacteria plated out in blood agar was observed only when the Ab-3 was added to said culture media, sugesting that the anti-anti-anti-SpA (Ab-3) bound to the S. aureus and inhibited their ability to proliferate in culture. On the other hand, bacterial growth was observed when blanks or sera of chicks fed non-hyper-immune eggs were used because these samples lacked the protective Ab-3 [1]. ReferenceJustiz Vaillant AA, Akpaka PE, Smikle M, McFarlane-Anderson N (2012) In vitro Inhibition of Staphylococcus aureus isolates by anti-antiIdiotypic antibodies to Staphylococca protein (SpA). J Vaccines Vaccin 3:127. doi:10.4172/2157-7560.1000127.

Proper citation: Angel Justiz-Vaillant, Patrick E. Akpaka 2020. Protocol for study of the in vitro effect of purified serum anti-anti-anti-SpA antibodies (Ab-3) on cultures of Staphylococcus aureus.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjkekkte Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. They have both paracrine and autocrine function. Interleukins are also used in animal studies to investigate aspect related to clinical medicine.[1]Macrophages, large granular lymphocytes, B cells, endothelium, fibroblasts, and astrocytes secrete IL-1. T cells, B cells, macrophages, endothelium and tissue cells are the principal targets. IL-1 causes lymphocyte activation, macrophage stimulation, increased leukocyte/endothelial adhesion, fever due to hypothalamus stimulation, and release of acute phase proteins by the liver. It may also cause apoptosis in many cell types and cachexia.[2]Reference1. Zhu Z, Wang D, Jiao W, Chen G, Cao Y, Zhang Q, Wang J. Bioinformatics analyses of pathways and gene predictions in IL-1α and IL-1β knockout mice with spinal cord injury. Acta Histochem. 2017 Sep;119(7):663-670. [PubMed]2. Boraschi D, Bossu P, Macchia G, Ruggiero P, Tagliabue A. Structure-function relationship in the IL-1 family. Front. Biosci. 1996 Oct 01;1:d270-308. [PubMed]

Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-1 in human serum.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj2zkqf6 Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Immune cells and activated epithelial cells secrete IL-20. It acts on epithelial cells. It plays a vital role in the cellular communication between epithelial cells and the immune system under inflammatory conditions.[1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.

Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-20 in human serum.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj3kkqkw Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Th9, Th2, Th17, mast cells, NKT cells, and regulatory T cells produce interleukin-9. It enhances T-cell survival, mast cell activation and synergy with erythropoietin.[1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.

Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-9 in human serum.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj29kqh6 Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Monocytes produce IL-12. Its principal targets are T cells. It causes induction of Th1 cells. Besides, it is a potent inducer of interferon gamma production by T lymphocytes and NK cells. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.

Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-12 in human serum.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj3bkqin Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Th2 lymphocytes synthesize IL-19 and acts on resident vascular cells in addition to immune cells. It is an anti-inflammatory molecule. It promotes immune responses mediated by regulatory lymphocytes and has substantial activity on microvascular.[1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.

Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-19 in human serum or plasma.. protocols.io https://dx.doi.org/10.17504/protocols.io.bkeqktdw Copy   

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Authors: Angel Justiz-Vaillant
Summary: This reagent can be used in ELISA, Western blotting and Dot blot to detect antigens and antibodies. It is important in the immunodiagnosis of infectious diseases and other problems. Protein L binds to kappa light chains of immunoglobulins from many animal species including human, mouse, rat, chicken, hamster and pig [1].1. Justiz-Vaillant AA, Akpaka PE, McFarlane-Anderson N, Smikle MF. Comparison of techniques of detecting immunoglobulin-binding protein reactivity to immunoglobulin produced by different avian and mammalian species.West Indian Med J. 2013;62(1):12-20.

Proper citation: Angel Justiz-Vaillant 2020. Preparation of horseradish peroxidate (HRP) conjugated Peptostreptococcal protein-L  by the periodate method.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjkkkkuw Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: A novel method was designed for the purification of Ab-2 from undiluted WSF of hyperimmune eggs. A total of six preparations were made.

Proper citation: Angel Justiz-Vaillant 2020. Purification of anti-anti-SpA antibody (Ab-2) using SpA-bearing Staphylococcus aureus cells.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjj9kkr6 Copy   

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Authors: Angel Justiz-Vaillant
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. [1]The immunoregulatory cytokine IL-41 (also known as meteorin-like protein) is expressed at high levels in the synovium of patients with psoriatic arthritis (PsA).[2]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.2.Onuora S. Novel cytokine, IL-41, linked with PsA.Nat Rev Rheumatol. 2019;15(11):636. doi:10.1038/s41584-019-0314-7

Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-4 in human serum.. protocols.io https://dx.doi.org/10.17504/protocols.io.bkbrksm6 Copy   

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