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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=12879439
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Cryopreserved Sperm (as of 2017-04-19)
Alternate IDs: 12879439
Notes: This double transgenic strain was established by co-injection of CAG-EGFP and mouse Acr-EGFP into F344/NSlc at Osaka University (over 5 generations, Dec 2016). There are two lines; GBGS(green body, green sperm)#2 and GBGS #6, but copy numbers and insertion site are not identified. Both lines show enough intensity of GFP fluorescence (no quantitative data). National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_12879439 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=13792822
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 13792822
Notes: The Sleeping Beauty transposon system was used to introduce a Drp1-GFP fusion driven by a ubiquitous promoter, interupted by a floxed stop cassette.This is line B. Contact MCW rat distribution at [email protected]
Proper citation: RRID:RGD_13792822 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=13792823
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 13792823
Notes: The Sleeping Beauty transposon system was used to introduce a Drp1-GFP fusion driven by a ubiquitous promoter, interupted by a floxed stop cassette.This is line C. Contact MCW rat distribution at [email protected]
Proper citation: RRID:RGD_13792823 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=150429988
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 150429988
Notes: This strain was derived by microinjecting fertilized eggs with a mix of the Sleeping Beauty construct containing BN Wars2 cDNA under control of the universal EF-1α (human EEF1A1) promoter and mRNA of the SB100X transposase (Ivics et al. 2014). Transgenic rats were detected using PCR with the following primers: Wars2-F 5'-TGT GCT ACA AGT CCA CAC AC-3' and Wars2-R 5'-GCA GAA GGG TCA CGA AGA GA-3'.
Proper citation: RRID:RGD_150429988 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=126848777
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Live Animals (as of 2021-04-30)
Alternate IDs: 126848777
Notes: Hras128 is a transgenic rat, introduced human proto-type c-Ha-ras gene to Jcl:SD rat fertilized egg by Dr. Hiroyuki Tsuda, Natl. Cancer Center, in 1998. The strain has been kept by CLEA Japan from its creation and supplied to the related research organization after concluded an agreement with Natl. Cancer Center and Japan Science and Technology Agency (JST). This strain is carrying three copies of the human c-Ha-ras proto-oncogene, including its own promoter region. CLEA Japan, Inc
Proper citation: RRID:RGD_126848777 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=126848778
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Live Animals (as of 2021-04-30)
Alternate IDs: 126848778
Notes: The transgene consisting of CAG promoter/loxP/neomycin resistant gene casette/lox/Ha-Kras*G12V (KrasV12) was injected into embryos of SD rat (RGD:8547938). This strain was generated at CLEA Japan,Inc. This strain is introduced 3 copies of hemagglutinin (HA) epitope tag connected HA-K-rasv12 gene expression cassette by using Cre/loxP system for controlling term/location expression of human mutated form K-rasv12 gene (changing glycine to valine at the 12 amino acid sequence) that normally floxed rat does not express K-rasv12 gene. CLEA Japan, Inc
Proper citation: RRID:RGD_126848778 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=42722004
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 42722004
Notes: Noninfectious clone containing gag-pol-deleted HIV-1 provirus under the viral LTR promoter was microinjected into fertilized one-cell Sprague�Dawley � Fisher 344/NHsd F1. Female founder with opaque cataracts was mating with wil-type Fischer 344/NHsd.
Tg rats from line 1 contained 20�25 copies.
Proper citation: RRID:RGD_42722004 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=38676446
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Cryopreserved Sperm (as of 2020-09-17)
Alternate IDs: 38676446
Notes: This strain was established by Bac transgenic method (construct: Kazuhiro Yamakawa, phD) supported by C.B.S.Nresource (Dr. Kazuto Kobayashi, Dr. Yuchio Yanagawa) in 2014 and maintained at Jikei University School of Medicine. Background: Crlj:WI. Transgene: VGAT (Slc32a1: mouse, Kanamycin registance gene (E. coli), Cre (P1 phage), polyA signal (SV40 virus. Mouse BAC clone: RP23-392-P11). Cre recombinase is expressed under the control of mouse Slc32a1 (VGAT) promoter. This strain is used for GABAergic neuron-specific Cre-Lox site-specific recombination system. National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_38676446 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=149735557
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Cryopreserved Sperm (as of 2024-01-26)
Alternate IDs: 149735557
Notes: This strain was produced by injecting a modified BAC, in which a Cre recombinase was inserted into the third exon of the rat Pdyn gene, into rat zygotes (Iar:Long-Evans, RGD:18337282). This strain is a transgenic rat that expresses Cre recombinase under the control of the Pdyn gene. National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_149735557 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=39128167
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Live Animals; Cryopreserved Sperm (as of 2020-09-23)
Alternate IDs: 39128167
Notes: This strain was generated by microinjecting YFP* (Venus): yellow fluorescent protein variant, which is inserted at downstream of Grp-promoter (4 kb) to Wistar rat zygotes. The number of inserted gene is approximately 100 copy. National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_39128167 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=150429614
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 150429614
Notes: A full-length rat wild type Add3 cDNA obtained from an expression plasmid pCMV6-entry-Add3 purchased from Origene (Rockville, MD) was inserted in a sleeping beauty transposon vector. The expression of wild type ADD3 in the transposon vector was driven
by a CAG promoter. The construct was injected into the pronucleus ofoocytes collected from female FHH/EurMcwi rats along with SB100 transposase mRNA. A single-transgene insertion was identified on chromosome 10, which is located .64 kbp away from the protein shisa-6 homolog precursor at its 59 end and .360 kbp away from the phosphoinositide-interacting protein at the 3 prime end. Heterozygous founders were intercrossed to derive a homozygous transgenic line that was used for studies.
Proper citation: RRID:RGD_150429614 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=38676447
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Cryopreserved Sperm (as of 2020-09-17)
Alternate IDs: 38676447
Notes: This strain was established by Bac transgenic method (construct: Kazuhiro Yamakawa, phD) supported by C.B.S.Nresource (Dr. Kazuto Kobayashi, Dr. Yuchio Yanagawa) in 2014 and maintained at Jikei University School of Medicine. Background: Crlj:WI. Transgene: VGAT (Slc32a1: mouse, Kanamycin registance gene (E. coli), Cre (P1 phage), polyA signal (SV40 virus. Mouse BAC clone: RP23-392-P11). Cre recombinase is expressed under the control of mouse Slc32a1 (VGAT) promoter. This strain is used for GABAergic neuron-specific Cre-Lox site-specific recombination system. National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_38676447 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=38676445
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Cryopreserved Sperm (as of 2020-09-17)
Alternate IDs: 38676445
Notes: This strain was established by Bac transgenic method (construct: Kazuhiro Yamakawa, phD) supported by C.B.S.Nresource (Dr. Kazuto Kobayashi, Dr. Yuchio Yanagawa) in 2014 and maintained at Jikei University School of Medicine. Background: Crlj:WI. Transgene: VGAT (Slc32a1: mouse, Kanamycin registance gene (E. coli), Cre (P1 phage), polyA signal (SV40 virus. Mouse BAC clone: RP23-392-P11). Cre recombinase is expressed under the control of mouse Slc32a1 (VGAT) promoter. This strain is used for GABAergic neuron-specific Cre-Lox site-specific recombination system. National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_38676445 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=35668863
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 35668863
Notes: This strain was made by pronuclear injection into LEW/Crl embryos. The embryos were co-injected with DNA fragments containing the HLA-B*2705 human gene and the human beta-2-microglobulin gene. The strain carries 40 copies of HLA-B*2705 and 30 copies of human beta-2-microglobulin gene. Founder 21-3 was selected and carrier animals from this founder were mated and bred to homozygosity.
Proper citation: RRID:RGD_35668863 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=15045606
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 15045606
Notes: This transgenic strain line Tg12085 carries the dual-reporter LacZ/EGFP (enhanced green fluorescent protein) cloned into the rat Prnp vector (raPrnp) and the expression of transgenes were driven by rat Prnp promoter.
Proper citation: RRID:RGD_15045606 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=14981597
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 14981597
Notes: SD rats were microinjected with a linear 12 kb EcoRI-BamH1 fragment containing the coding sequence and promoter region of human SOD1 gene with G93A mutation. Originally created by John Kulik at Wyeth. Transgenic Model of SOD1 mediated Amyotrophic Lateral Sclerosis (ALS, Lou Gehrig's Disease). Came to Taconic in April 2002 for David Howland at Wyeth for distribution as an Emerging Model. Joint collaboration between Wyeth and The ALS Association. TACONIC
Proper citation: RRID:RGD_14981597 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=151356985
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 151356985
Notes: This LE transgenic rats were created by injecting the cfos-eGFP construct to pronuclei of fertilized eggs obtained from Long Evans females. The 5 prime end of the transgenic DNA construct contained mouse genomic cFos (indluding the promoter and all exon and intron sequences) was fused with eGFP (including poly(A) tail derived from the IRES (phosphorylated-internal ribosomal entry site) of the EGFP Clontech (Cambridge, UK) vector. A single founder animal was identified. All subsequent breeding used hemizygous cfos-GFP male rats paired with wild-type Long Evans female rats obtained from Charles River Laboratories. Rats were bred on Long-Evans background for 13 generations at NIDA IRP (Hope lab).
Proper citation: RRID:RGD_151356985 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=38599204
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Cryopreserved Sperm (as of 2020-09-15)
Alternate IDs: 38599204
Notes: The transgenic LE line 2(Back ground strain: Iar:Long-Evans (RGD:18337282) (Kumagai-shigeyasu Co.,Ltd)) carrying Transgene: ROSA26 (mouse ROSA26 BAC: RP23 244D9), and Channel rhodopsin fast receiver (ChRFR)(COP4*C167A) (Chlamydomonas reinhardtii) fused with YFP* (Venus). Copy number: 1, In this strain, COP4*C167A, modified photosensitivity cation channel, is expressed ubiquitously in the presence of exogenous Cre protein. The ChRFR(COP4*C167A), one of the channel rhodopsin (step-function opsin, SFO), opens under blue light and is closed under yellow light. The Venus (YFP*) is tagged with channel rhodopsin. There is no differences between line 1 and 2 except for copy number. National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_38599204 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=38599203
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Cryopreserved Sperm (as of 2020-09-15)
Alternate IDs: 38599203
Notes: The transgenic LE line 2(Back ground strain: Iar:Long-Evans (RGD:18337282) (Kumagai-shigeyasu Co.,Ltd)) carrying Transgene: ROSA26 (mouse ROSA26 BAC: RP23 244D9), and Channel rhodopsin fast receiver (ChRFR)(COP4*C167A) (Chlamydomonas reinhardtii) fused with YFP* (Venus). Copy number: 3, In this strain, COP4*C167A, modified photosensitivity cation channel, is expressed ubiquitously in the presence of exogenous Cre protein. The ChRFR(COP4*C167A), one of the channel rhodopsin (step-function opsin, SFO), opens under blue light and is closed under yellow light. The Venus (YFP*) is tagged with channel rhodopsin. There is no differences between line 1 and 2 except for copy number. National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_38599203 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=151356741
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 151356741
Notes: This transgenic strain was generated by microinjection into outbred Sprague-Dawley from Harlan Sprague-Dawley (Hsd:SD) fertilized eggs and carries simian virus T Antigen drived by the enhancer/promoter region of the mouse albumin gene.
Proper citation: RRID:RGD_151356741 Copy
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