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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=14394620
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 14394620
Notes: The Tph2em2Mcwi was produced by injecting ZFNs targeting the sequence CATGGCTCCGACCCCctctacACCCCGGAACCG into DA/OlaHsd rat embryos. The resulting mutation is a 10-bp frameshift deletion in exon 7.
Proper citation: RRID:RGD_14394620 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=14394619
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 14394619
Notes: This wild type strain was produced by crossing Tph2em2Mcwi heterozygous rats and confirmed by sequencing. The Tph2em2Mcwi was produced by injecting ZFNs targeting the sequence CATGGCTCCGACCCCctctacACCCCGGAACCG into DA/OlaHsd rat embryos. The resulting mutation is a 10-bp frameshift deletion in exon 7.
Proper citation: RRID:RGD_14394619 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=14394618
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 14394618
Notes: This homozygous mutant strain was produced by crossing Tph2em3Mcwi heterozygous rats and confirmed by sequencing. The Tph2em3Mcwi heterozygous mutant was created by injecting ZFNs targeting the sequence CATGGCTCCGACCCCctctacACCCCGGAACCG into DA/OlaHsd rat embryos. The resulting mutation is a 11-bp frameshift deletion in exon 7.
Proper citation: RRID:RGD_14394618 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=126908010
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 126908010
Notes: The homozygous Kcnk3 mutant rats are littermates of cross of heterozygotesKcnk3 mutant rats . The mutation created by CRISPR/Cas9 contained a 94- bp deletion in exon1 of resulted a premature stop codon.
Proper citation: RRID:RGD_126908010 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=126908013
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 126908013
Notes: The heterozygous Kcnk3 mutant rats were created by CRISPR/Cas9. The mutated allele contained a 94- bp deletion in exon1 of resulted a premature stop codon.
Proper citation: RRID:RGD_126908013 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=149735336
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 149735336
Notes: Rat Wfs1 exon 5-specific zinc-finger nucleases (ZNFs) and microinjection-ready mRNA were injected to embryos harvested from female Sprague-Dawley rats (Crl: CD(SD) )rats. Thereafter, microinjected egg cells were transferred to the oviduct of pseudopregnant Sprague-Dawley recipients.Three different Wfs1 mutant rat lines were created: Wfs1em1 ( Wfs1-ex5-KO232), Wfs1em2 (Wfs1-ex5-KO266) and Wfs1em3 (Wfs1-ex5-INS244). Wfs1em2 rats carry a 27 amino acids deletion in exon 5 (aa212-238).
Proper citation: RRID:RGD_149735336 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=14394487
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 14394487
Notes: The Bace1 (+/+) rats were generated by crossing SD-Bace1em1Sage. The mutant rats were generated by SAGE Labs using zinc-finger nuclease (ZFN) technology to create a 137-base pair deletion spanning the translation initiation start site in exon 1 of the rat Bace1 gene, corresponding to chr8:48,766,315-48,766,452 (RGSC 5.0/rn5 assembly)
Proper citation: RRID:RGD_14394487 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=38549343
Source Database: Rat Genome Database (RGD)
Genetic Background: inbred
Availability: Unknown
Alternate IDs: 38549343
Notes: A substrain of WKY from Charles River France and bred at Institut Francois Magendie, Bordeaux Cedax, France. Charles River, France
Proper citation: RRID:RGD_38549343 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=126790547
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 126790547
Notes: This strain was produced by injecting TALEN targeting the sequence of ratRin1 into SD embryos. The resulting mutation is a knock out of the gene.
Proper citation: RRID:RGD_126790547 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=150429965
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 150429965
Notes: TALEN system targeting the rat Apoa4 gene was injected to Sprague Dawley embryos. An 8 bp deletion was induced within the coding region of Apoa4 gene, which results in a frameshift and gene knockout. The genotypes were confirmed by PCR analysis followed by Sanger sequencing and agarose electrophoresis (PCR forward primer: 5′-TATCCCAACTCCAACATCATCCA-3′, reverse primer: 5′-TCGCAGTCTGATCCCACTTACTT-3′). The knockout allele generated a band at 237 bp, while the wild-type allele was at 245 bp.
Proper citation: RRID:RGD_150429965 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=150520185
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 150520185
Notes: Zygotes of SD rats were microinjected with CRISPR/Cas9 system targeting Ldlr . This mutant possesses a 118-bp deletion from No.22759599bp to 22759716bp in the Ldlr gene (NC_005107.4), resulting in a termination codon TAG, and deletion
of 768 amino acids of Ldlr.
Proper citation: RRID:RGD_150520185 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=150520221
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 150520221
Notes: The Sprague Dawley embryos were injected with CRISPR/Case9 system targeting the first exon of the rat Slco1b2 gene. A Slco1b2 knockout was created with 11-bp deletion.
Proper citation: RRID:RGD_150520221 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=150520189
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 150520189
Notes: The mutant rat strain was produced by injecting TALEN system targeting the exon 4 of rat Apoe into Sprague Dawley (Hsd:SD) embryos. This mutant rat has a 16-bp deletion in the gene third coding exon) located on chromosome 1; frameshift mutation resulted (p.Glu178fs)
Proper citation: RRID:RGD_150520189 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=38543943
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 38543943
Notes: The Rag2 gene in rat spermatogonia stem cells (SSC, SD-WT2) was targeted by TALENs and resulted in a 27-bp in-frame deletion. The genome modified SSC was transplanted to Busulfan-treated, Dazl-deficient male Sprague Dawley rats and mated with wild type female Sprague Dawley rats 70 to 81 later. The allele was then bred to homozygosity to generate the Sprague-Dawley Rag2 (SDR)-knockout rat.
Proper citation: RRID:RGD_38543943 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=38549351
Source Database: Rat Genome Database (RGD)
Genetic Background: recombinant_inbred
Availability: Unknown
Alternate IDs: 38549351
Notes: Embryo-rederived from breeder stock provided by Pravenec and Kren from the Prague colonies, and maintain by Medical College of Wisconsin.
Proper citation: RRID:RGD_38549351 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=38549356
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 38549356
Notes: CRISPR/Cas9 system was used to generate this double-gene knockout. The Slc:SD zygotes were injected with sgRNAs that containing 4 sgRNAs (5 ng/ml each) targeting 2 adjacent sites (target sites 1 and 2) of each of the 2 genes. The resulting mutations are 317 bp (Defb23) and 380 bp (Defb26) fragments which were deleted in the Defb23/Defb26 double-mutant strain.
Proper citation: RRID:RGD_38549356 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=150520215
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 150520215
Notes: CRISPR/Cas9 was used to delete miR1208 in a 763bp excision in ACI rat embryos. This strain is maintained at Rat Resource and Research Center.
Proper citation: RRID:RGD_150520215 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=150520216
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Live Animals (as of 2021-11-05)
Alternate IDs: 150520216
Notes: Using CRISPR/Cas9, a suspected enhancer of miR1208 was deleted in a 8315bp excision in ACI rat embryos.
Proper citation: RRID:RGD_150520216 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=150520217
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 150520217
Notes: Using CRISPR/Cas9, a suspected enhancer of miR1208 was deleted in a 8315bp excision in ACI rat embryos. This strain is maintained at Rat Resource and Research Center.
Proper citation: RRID:RGD_150520217 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=150429615
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown (as of 2021-09-09)
Alternate IDs: 150429615
Notes: ZFN system was used to Knock out the rat Add3 gene of Crl:SD rat embryos.The ZFN mRNA was injected into the pronucleus of fertilized SpragueDawley embryos and transferred to the oviduct of pseudopregnant females. PCR genotyping of tail biopsies confirmed a 14-bp deletion using forward primer 5'-GCCCCCATGAGTCACTACAC-3' and reverse primer 5'-GCTACAGGAAGCATCTCCTGTG-3'. Founders with Add3 deletion were backcrossed to the parental strain to generate heterozygous F1 rats. Heterozygous F1 siblings were then intercrossed to derive a homozygous KO line used
Proper citation: RRID:RGD_150429615 Copy
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