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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00062776
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: myls13; oyIs51.
Notes: Made_by: Abby Poff|"myls13 [klp-6p::GFP]. oyIs51 [srh-142::RFP]. IL2 neurons are marked with GFP. ADF neurons are marked with RFP. Derived from parental line PY3470. Can be used to isolate IL2 neurons (GFP) and ADF neurons (RFP) by FACS. Used by CeNGEN project for RNA-Seq (https:"
Proper citation: RRID:WB-STRAIN:WBStrain00062776 Copy
http://www.wormbase.org/db/get?name=WBStrain00062803
Source Database: WormBase (WB)
Affected Genes: WBGene00001864(him-5)
Genomic Alteration: WBGene00001864(him-5)
Availability: unknown
Source References: EMPTY
Synonyms: him-5(e1490) V; otEx7231.
Notes: Made_by: Maryam Majeed|"otEx7231 [srg-8p::rab-3::GFP + srg-8p::mCherry]. Pick animals with mCherry expression to maintain. Presynaptic RAB-3::GFP reporter for ASK neurons with cytoplasmic ASKp::mCherry in the background. Reference: Majeed M, et al. Elife. 2024 Jan 15:12:RP91775. doi: 10.7554/eLife.91775. PMID: 38224479."
Proper citation: RRID:WB-STRAIN:WBStrain00062803 Copy
http://www.wormbase.org/db/get?name=WBStrain00062809
Source Database: WormBase (WB)
Affected Genes: WBGene00019994(cdh-1)
Genomic Alteration: WBGene00019994(cdh-1)
Availability: unknown
Source References: EMPTY
Synonyms: cdh-1(ot1034) III.
Notes: CRISPR/Cas9 engineered deletion of full cdh-1 locus.|"Made_by: Maryam Majeed"
Proper citation: RRID:WB-STRAIN:WBStrain00062809 Copy
http://www.wormbase.org/db/get?name=WBStrain00062806
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: otEx7501.
Notes: Made_by: Steven J Cook|"otEx7501 [pdfr-1::GFP::cla-1 + pdfr-1::tagRFP + rol-6(su1006)]. Pick Rollers to maintain. Presynaptic GFP::cla-1 reporter for pharyngeal I1 neurons. Reference: Cook SJ, et al. J Comp Neural. 2020 Nov 1;528(16):2767-2784. doi: 10.1002/cne.24932. PMID: 32352566."
Proper citation: RRID:WB-STRAIN:WBStrain00062806 Copy
http://www.wormbase.org/db/get?name=WBStrain00062763
Source Database: WormBase (WB)
Affected Genes: WBGene00001135(eat-4)
Genomic Alteration: WBGene00001135(eat-4)
Availability: unknown
Source References: EMPTY
Synonyms: mirSi16 II; eat-4(mir12[loxP 5'UTR] mir17[loxP intron2]) III; mirIs47.
Notes: mirSi16 [flp-18p::lox2272::BFP::tbb-2 3'UTR::lox2272::ChR2-HRDC::SL2::jRGECO1a::unc-54 3'UTR + Cbr-unc-119(+)] II. mirIs47 [sra-6p::TeNL + npr-9p::ChR2-HRDC::YFP::jRGECO1a + unc-119(+) + gpa:14p::CRE]. Blue fluorescence in flp-18 expressing neurons. loxP sites before first (eat-4(mir12[loxP 5'UTR])) and after second exon (eat-4(mir17[loxP intron2])) of eat-4 gene. Lite. Calcium sensitive (Kd 250 nM) teal nanolantern (TeNL) in ASH and PVQ, ChR2-HRDC::YFP and jRGECO1a expression in AIB. Conditional eat-4 KO in ASH (NOT defective) and ChR2-HRDC and jRGECO1a expression in AVA (instead of BFP). Reference: Porta-de-la-Riva M, et al. Nat Methods. 2023 May;20(5):761-769. doi: 10.1038/s41592-023-01836-9. PMID: 37024651.
Proper citation: RRID:WB-STRAIN:WBStrain00062763 Copy
http://www.wormbase.org/db/get?name=WBStrain00062764
Source Database: WormBase (WB)
Affected Genes: WBGene00001135(eat-4)|WBGene00001803(lite-1)
Genomic Alteration: WBGene00001135(eat-4), WBGene00001803(lite-1)
Availability: unknown
Source References: EMPTY
Synonyms: eat-4(mir12[loxP 5'UTR] mir17[loxP intron2]) III; lite-1 (ce314) X.
Notes: loxP sites inserted before first (eat-4(mir12[loxP 5'UTR])) and after second (eat-4(mir17[loxP intron2])) exon of eat-4 gene. Lite. Reference: Porta-de-la-Riva M, et al. Nat Methods. 2023 May;20(5):761-769. doi: 10.1038/s41592-023-01836-9. PMID: 37024651.
Proper citation: RRID:WB-STRAIN:WBStrain00062764 Copy
http://www.wormbase.org/db/get?name=WBStrain00062762
Source Database: WormBase (WB)
Affected Genes: WBGene00044630(bus-17)
Genomic Alteration: WBGene00044630(bus-17)
Availability: unknown
Source References: EMPTY
Synonyms: bus-17(br2) X; mirEx123.
Notes: mirEx123 [myo-3p::TeNL::unc-54 3' UTR]. Maintain strain by picking animals with blue fluorescence. bus-17(br2) has mutant defective response to short wavelength light; response strongly reduced but not eliminated. Altered surface properties; somewhat skiddy movement; drug-sensitive, bleach-sensitive. Resistant to some bacterial pathogens (hence Bus, Bah phenotypes) and hypersensitive to others. Calcium sensitive (Kd 250 nM) teal nanolantern (TeNL) in muscles. Reference: Morales-Curiel LF, et al. Commun Biol. 2022 Dec 3;5(1):1330. doi: 10.1038/s42003-022-04292-x. PMID: 36463346.
Proper citation: RRID:WB-STRAIN:WBStrain00062762 Copy
http://www.wormbase.org/db/get?name=WBStrain00062767
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: mirSi29 II; unc-119(ed3) III.
Notes: Made_by: Neus Sanfeliu Cerdan|"mirSi29 [flp-18p::lox2272::mtagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR + Cbr-unc-119(+)] II. Blue fluorescence in flp-18 expressing neurons. Combine with CRE driven by a promoter co-expressed in flp-18 expressing cells to switch from blue expression to ACR1 and jRGECO1a expression. Reference: Porta-de-la-Riva M, et al. Nat Methods. 2023 May;20(5):761-769. doi: 10.1038/s41592-023-01836-9. PMID: 37024651."
Proper citation: RRID:WB-STRAIN:WBStrain00062767 Copy
http://www.wormbase.org/db/get?name=WBStrain00062800
Source Database: WormBase (WB)
Affected Genes: WBGene00000898(daf-2)|WBGene00002162(isp-1)
Genomic Alteration: WBGene00000898(daf-2), WBGene00002162(isp-1)
Availability: unknown
Source References: EMPTY
Synonyms: daf-2(e1370) III; isp-1(qm150) IV/nT1[qIs51] (IV;V).
Notes: Maintain at 15-20C: will form dauers at higher temps. Slow growing. Pick GFP+ to maintain. Heterozygotes are GFP+ and segregate WT GFP+ hets, arrested nT1[qIs51] aneuploids, and non-GFP daf-2(e1370); isp-1(qm150) homozygotes (sterile). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. [NOTE: Originally published daf-2(e1370); isp-1(qm150) strain MQ876 was found to not actually contain the daf-2(e1370) mutation. This strain was made to study the double mutant and revealed that daf-2(e1370); isp-1(qm150) homozygotes are inviable/sterile.] Reference: Cooper et al. In preparation.
Proper citation: RRID:WB-STRAIN:WBStrain00062800 Copy
http://www.wormbase.org/db/get?name=WBStrain00062768
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: mirSi34 II; unc-119(ed3) III.
Notes: Made_by: Neus Sanfeliu Cerdan|"mirSi34 [myo-3p::lox2272::mtagBFP2::tbb-2 3'UTR::lox2272::ChR2-HRDC::SL2::jRGECO1a::let-858 3'UTR + Cbr-unc-119(+)] II. Blue fluorescence in body wall muscles. Combine with CRE driven by a promoter expressed in desired muscle cells to switch from blue expression to ChR2-HRDC and jRGECO1a expression. Reference: Porta-de-la-Riva M, et al. Nat Methods. 2023 May;20(5):761-769. doi: 10.1038/s41592-023-01836-9. PMID: 37024651."
Proper citation: RRID:WB-STRAIN:WBStrain00062768 Copy
http://www.wormbase.org/db/get?name=WBStrain00062765
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: mirSi16 II; mirIs76; mirEx69.
Notes: mirSi16 [flp-18p::lox2272::BFP::tbb-2 3'UTR::lox2272::ChR2-HRDC::SL2::jRGECO1a::unc-54 3'UTR + Cbr-unc-119(+)] II. mirIs76 [sra-6p::ChRmine::wrmScarlet::let-858 3UTR + sra-6p::TeNL]. mirEx69 [gpa:14p::CRE + unc-122p::mCherry]. Mantain by picking animals with mCherry+ coelomycetes. Blue in flp-18 expressing neurons. Expression of ChRmine, wrmScarlet and calcium sensitive (Kd 250 nM) teal nanolantern (TeNL) in ASH and PVQ. Expression of ChR2-HRDC and jRGECO1a expression in AVA (instead of BFP). Reference: Porta-de-la-Riva M, et al. Nat Methods. 2023 May;20(5):761-769. doi: 10.1038/s41592-023-01836-9. PMID: 37024651.
Proper citation: RRID:WB-STRAIN:WBStrain00062765 Copy
http://www.wormbase.org/db/get?name=WBStrain00062758
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: mirSi16 II; unc-119(ed3) III; mirIs110 V; mirIs107.
Notes: mirSi16 [flp-18p::lox2272::BFP::tbb-2 3'UTR::lox2272::ChR2-HRDC::SL2::jRGECO1a::unc-54 3'UTR + Cbr-unc-119(+)] II. mirIs110 [odr-7p::TeNL + unc-122p::GFP *oxTi553 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]] V. mirIs107 [gpa:14p::CRE + npr-9p::ChR-HRDC::YFP::SL2::jRGECO1a + rps-0p::hygroR]. Blue fluorescence in flp-18 expressing neurons. Green coelomycetes segregates with calcium sensitive (Kd 250 nM) teal nanolantern (TeNL) in AWA. ChR2-HRDC and jRGECO1a in AVA (instead of BFP) and ChR2-HRDC::YFP and jRGECO1a in AIB. HygroR. Reference: Porta-de-la-Riva M, et al. Nat Methods. 2023 May;20(5):761-769. doi: 10.1038/s41592-023-01836-9. PMID: 37024651.
Proper citation: RRID:WB-STRAIN:WBStrain00062758 Copy
http://www.wormbase.org/db/get?name=WBStrain00062759
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: mirSi16 II; unc-119(ed3) III.
Notes: mirSi16 [flp-18p::lox2272::BFP::tbb-2 3'UTR::lox2272::ChR2-HRDC::SL2::jRGECO1a::unc-54 3'UTR + Cbr-unc-119(+)] II. Blue fluorescence in flp-18 expressing neurons. Combine with CRE driven by a promoter co-expressed in flp-18 expressing cells to switch from blue expression to ChR2-HRDC and jRGECO1a expression. Reference: Porta-de-la-Riva M, et al. Nat Methods. 2023 May;20(5):761-769. doi: 10.1038/s41592-023-01836-9. PMID: 37024651.
Proper citation: RRID:WB-STRAIN:WBStrain00062759 Copy
http://www.wormbase.org/db/get?name=WBStrain00062750
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: unc-119(ed3) III; qbcSi12 IV.
Notes: qbcSi112 [elt-2p::GFP::his-58::3XmiR-228(mut)::tbb-2 3UTR + unc-119(+)] IV. Somatic miR-228 miRNA GFP reporter with a mutation in the miRNA-binding site. Reference: Dallaire et al. Dev Cell. 2018 Oct 22;47(2):239-247.e4. doi: 10.1016/j.devcel.2018.08.022. PMID: 30245155.
Proper citation: RRID:WB-STRAIN:WBStrain00062750 Copy
http://www.wormbase.org/db/get?name=WBStrain00062751
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: unc-119(ed3) III; qbcSi11 IV.
Notes: qbcSi11 [elt-1p::GFP::his-58::3XmiR-228::tbb-2 3UTR + unc-119(+)] IV. Somatic miR-228 miRNA GFP reporter. Reference: Dallaire et al. Dev Cell. 2018 Oct 22;47(2):239-247.e4. doi: 10.1016/j.devcel.2018.08.022. PMID: 30245155.
Proper citation: RRID:WB-STRAIN:WBStrain00062751 Copy
http://www.wormbase.org/db/get?name=WBStrain00062756
Source Database: WormBase (WB)
Affected Genes: WBGene00044630(bus-17)
Genomic Alteration: WBGene00044630(bus-17)
Availability: unknown
Source References: EMPTY
Synonyms: bus-17(br2) X; mirIs92.
Notes: mirIs92 [daf-16p::daf-16::GcNL::let-858 3'UTR + myo-2p::mCherry]. bus-17(br2) has defective response to short wavelength light; response strongly reduced but not eliminated. Altered surface properties; somewhat skiddy movement; drug-sensitive, bleach-sensitive. Resistant to some bacterial pathogens (hence Bus, Bah phenotypes) and hypersensitive to others. daf-16 translational reporter with green non-calcium sensitive nanolantern (GCNL). Reference: Morales-Curiel LF, et al. Commun Biol. 2022 Dec 3;5(1):1330. doi: 10.1038/s42003-022-04292-x. PMID: Reference: Morales-Curiel LF, et al. Commun Biol. 2022 Dec 3;5(1):1330. doi: 10.1038/s42003-022-04292-x. PMID: 36463346.
Proper citation: RRID:WB-STRAIN:WBStrain00062756 Copy
http://www.wormbase.org/db/get?name=WBStrain00062754
Source Database: WormBase (WB)
Affected Genes: WBGene00003776(nmy-1)
Genomic Alteration: WBGene00003776(nmy-1)
Availability: unknown
Source References: EMPTY
Synonyms: nmy-1(mc90[nmy-1::gfp]) X.
Notes: Maintain at 15C. nmy-1(mc90) allele induces almost complete sterility at 25C. mc90 is a CRISPR-engineered mutation in nmy-1 at the position corresponding to the allele nmy-2(ne3409) changing NMY-2 Leucine-981 to Proline, which is conserved among nonmuscle myosins. mc90 was introduced in parental strain ML2540, which carries a GFP-tag in the endogenous nmy-1 locus. Reference: Molnar K, et al. Genetics. 2024 Sep 4;228(1):iyae109. doi: 10.1093/genetics/iyae109. PMID: 39053622.
Proper citation: RRID:WB-STRAIN:WBStrain00062754 Copy
http://www.wormbase.org/db/get?name=WBStrain00062755
Source Database: WormBase (WB)
Affected Genes: WBGene00003776(nmy-1)|WBGene00003777(nmy-2)
Genomic Alteration: WBGene00003776(nmy-1), WBGene00003777(nmy-2)
Availability: unknown
Source References: EMPTY
Synonyms: nmy-2(ne3409) I; nmy-1(mc90[nmy-1::gfp]) X.
Notes: Maintain at 15C. nmy-2(ne3409) allele induces embryonic lethality (cytokinesis defective) at 25C. nmy-1(mc90) allele induces almost complete sterility at 25C. mc90 is a CRISPR-engineered mutation in nmy-1 at the position corresponding to the allele nmy-2(ne3409) changing NMY-2 Leucine-981 to Proline, which is conserved among nonmuscle myosins. mc90 was introduced in parental strain ML2540, which carries a GFP-tag in the endogenous nmy-1 locus. Reference: Molnar K, et al. Genetics. 2024 Sep 4;228(1):iyae109. doi: 10.1093/genetics/iyae109. PMID: 39053622.
Proper citation: RRID:WB-STRAIN:WBStrain00062755 Copy
http://www.wormbase.org/db/get?name=WBStrain00062749
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: unc-119(ed3) III; qbcSi10 IV.
Notes: qbcSi10 [mex-5p::GFP::his-58::3XmiR-228(mut)::tbb-2 3UTR + unc-119(+)] IV. Germline-specific miR-228 miRNA GFP reporter with a mutation in the miRNA-binding site. Reference: Dallaire et al. Dev Cell. 2018 Oct 22;47(2):239-247.e4. doi: 10.1016/j.devcel.2018.08.022. PMID: 30245155.
Proper citation: RRID:WB-STRAIN:WBStrain00062749 Copy
http://www.wormbase.org/db/get?name=WBStrain00062747
Source Database: WormBase (WB)
Affected Genes: WBGene00001330(eps-8)
Genomic Alteration: WBGene00001330(eps-8)
Availability: unknown
Source References: EMPTY
Synonyms: eps-8(cp441[eps-8::mNG-C1]) IV.
Notes: Made_by: Pu Zhang|"mNG reporter inserted into endogenous eps-8 locus. Reference: Zhang P, et al. 2023 Sep 4;222(9):e202302102. doi: 10.1083/jcb.202302102. PMID: 37351566.."
Proper citation: RRID:WB-STRAIN:WBStrain00062747 Copy
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