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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[3’neo2, HAn]; pm-r, VII)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal HA tag of HHT3, not sure if complete replacement
Source References: PMID:9343391, PMID:16908532
Notes: HA-tagged hv2 transformant. Checked by western blot and immunofluorescent staining
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02648 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: eri1::neo3/eri1::neo3; mpr1-1/mpr1-1 (eri1::neo3; pm-r, ?)
Affected Genes: ERI1 (TTHERM_00501020)
Genomic Alteration: Micronucleus: ERI1 coding replaced by neo3 Macronucleus: ERI1 coding replaced by neo3
Notes: homozygous Er1 ko homokaryon ‘a’ can be mating with homozygous Er1 ko homokaryon ‘d’; coding sequences were replaced by Neo 3. Developmental delay.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02777 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 3R (CHX1; pmr1-1; cy-s, pm-r, II)
Notes: Deletion on right arm of chromosome 3.
Proper citation: RRID:TSC_SD00994 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: drh1[∆?::neo4]/drh1[∆?::neo4]
Affected Genes: DRH1 (TTHERM_00190830)
Genomic Alteration: Micronucleus: Neo4 into 5’ end of coding of DRH1
Notes: Neo4 KO cassette in DRH1 locus, homozygous KO in mic, mac assorted to pm-s. Two cell lines in row. Mate with D3, H7, and C10 cell lines. Homozygous heterokaryon.
Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryon KOs do not grow well.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02139 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: BTU1/btu1[∆,::H4-neo]; MPR1-1/mpr1-1
Affected Genes: BTU1 (TTHERM_00348510)
Genomic Alteration: Micronucleus: H4-neo replaces coding of BTU1, heterozygous
Source References: PMID:10831613, PMID:9037049
Notes: Germline transformant (heterozygote). Transformed with BHAB-1 (BTU1 knockout construct)
Mates with B1KO-1
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01840 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-?[E431D,E432D,E433D,E435D,E437D,E438D,E439D,E440D,E442D]; ATU1/atu1-1[α-β hybrid tail, AAB2]; pm-r, Original BTU2 is replaced by bsr1, BTU1 coding replaced by neo1, then transformed with BTU1 with (E431-442D) and Chimeric ATU1 gene, AAB2 (α-β hybrid ), ?)
Affected Genes: BTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580), ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: knockout of btu1 with neo
knockout of btu2 with bsr Macronucleus: BTU2 replaced by bsr. BTU1 partially replaced by neo1, some replaced by BTU1-D9 with (E431-442D). Some ATU1 replaced by ATU1 with chimeric α-β hybrid tail.
Source References: PMID:10831613, PMID:11864572, PMID:15254268
Notes: Progeny of DB6B x DB2A, co- transformed by constructs BTU1-D9 with (E431-442D) and construct of chimeric ATU1 gene, AAB2 (α-β hybrid ) See publications.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02021 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: drh1[∆?::neo4]/drh1[∆?::neo4]
Affected Genes: DRH1 (TTHERM_00190830)
Genomic Alteration: Micronucleus: Neo4 into 5’ end of coding of DRH1
Notes: Neo4 KO cassette in DRH1 locus, homozygous KO in mic, mac assorted to pm-s. Two cell lines in row. Mate with D3, H7, and C10 cell lines. Homozygous heterokaryon.
Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryon KOs do not grow well.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02140 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hho1[∆::neo2]; MPR1; pm-r, VII)
Affected Genes: HHO1 (TTHERM_00823720)
Genomic Alteration: Macronucleus: Neo2 into HHO1 locus
Source References: PMID:7606784
Notes: H1 knockout obtained by microinjection
mac transformant, parent is CU428.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02789 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaE-3/cdaE-3 (cdaE-3, II)
Affected Genes: CDAE (cell division arrest E)
Source References: PMID:821816, PMID:407114
Notes: Incomplete fission-zone formation with arrest in late-cytokinesis, with the new oral apparatus often abnormal and frequently wedged within the arrested furrow.
Expressed in many cells at normal growth temperatures, near 100% at 39°. Same phenotype as the allelic IA137 stock,but with stronger expression.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01458 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: janA-2/janA-2; eja1-1/eja1-1 (janA-2; eja1-1; janA, eja1, II)
Affected Genes: JANA (janus A) (originally reported as jan); EJA1 (enhancer of janus)
Source References: PMID:1743393
Notes: A mutation that causes the dorsal surface to be converted to a partial mirror-image of the ventral surface and blocks conjugation. Allelic to janA-1.
Proper citation: RRID:TSC_SD00643 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Affected Genes: (The calmodulin IES)
Source References: PMID:15037070
Notes: Submitted by P. Huvos, Southern Illinois Univesity
Proper citation: RRID:TSC_SD01734 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: fat2-1/fat2-1 (fat2-1, II)
Affected Genes: FAT2 (formerly FATB)
Notes: Somewhat pudgy but otherwise completely normal. Expression is not appreciably temperature-sensitive.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01221 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; Nulli 4 (MPR1; mp-s, VII)
Proper citation: RRID:TSC_SD00890 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 2R [B or C3] (pmr1-1; cnj3, pm-r, VII)
Proper citation: RRID:TSC_SD00379 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, HAc(::GTU1, A101G)]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations A101G and c-terminus HA tag into the MTT1 locus, partial replacement.
Source References: PMID:12356864, PMID:16344310
Notes: pGTUA101G-HA rescued GTUKO 5x6 progeny having mutation @ GTU1 A101G in GTU1-1 gene, driven by MTT1 promoter.
Grow and maintain in 0.5 ug/ml CdCl2 at 30C.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02043 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hpo1-1/hpo1-1; janC-1/janC-1 (hpo 1-1; janC-1; hpo1; janC, IV)
Affected Genes: HPO1 (hypoangular 1), JANC (janusC)
Notes: janC is very weakly expressed in this double homozygote.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01751 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1
(mtt1-?[5’neo2,∆::BLT1, HAc], VII)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Macronucleus: BLT1with c-terminal HA replaces MTTi coding with neo2 upsteam in Mtt1 5’flanking region
Notes: CU428 somatic replacement with BLT-HA tag in the MTT1 locus, has Neo2 in 5’ flanking region of MTT1. Wild type BLT1 gene still present. This is a partial replacement. Cannot single-cell, only some cells express the HA tagged BLT when induced with Cd.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02563 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: presumed phg-/phg- (presumed phg-; phg-, ?)
Source References: PMID:23195323, PMID:18658256, PMID:18311715
Notes: The Frankel lab could not obtain progeny from crosses using this stock. Its observations are consistent with the reported temperature-sensitive food-vacuoleless phenotype, but not consistent with the reported total loss of oral structures. This stock exhibits multiple unique morphogenetic phenotypes at 37.5o C.: see Frankel (2008: p. 1626, 1632). The Iowa stock is designated II8G-IA to distinguish it from the II8G stock, deposited by L. Rasmussen at the American Type Culture Collection. ATCC.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01631 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ∆HTB1/∆HTB1 pm-r with neo2 (pm-r, cy-s)
Affected Genes: HTB1 (TTHERM_00633360)
Notes: HTB1 complete KO strain.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01999 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ∆HHT1/∆HHT1; ∆HHT3/∆HHT3
hht1[∆::neo2]/hht1[∆::neo2];hht3[∆::neo2]/hht3[∆::neo2] (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT1 + HHT3 (TTHERM_00570560, TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo2 KO of HHT1 and HHT3
Source References: PMID:16908532
Notes: homozygous, Neo cassette knockout
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01879 Copy
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