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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00055277
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: syIs710; syIs300.
Notes: Made_by: Stephanie Nava/Shahla Gharib|"syIs710 [srg-13p::NLS::GAL4(sk)::VP64::let-858 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)]. cGAL driver for PHA neurons. syIs300 [15xUAS::(delta)pes-10::GFP::let-858 3'UTR + ttx-3p::RFP + 1kb DNA ladder(NEB)] is a GFP cGAL effector. Some worms do not express ttx-3p::RFP marker, but will consistently produce worms with the transgenic marker in next generation."
Proper citation: RRID:WB-STRAIN:WBStrain00055277 Copy
http://www.wormbase.org/db/get?name=WBStrain00055279
Source Database: WormBase (WB)
Affected Genes: WBGene00018141(oac-21)
Genomic Alteration: WBGene00018141(oac-21)
Availability: unknown
Source References: EMPTY
Synonyms: oac-21(sy1566) IV.
Notes: Made_by: Heenam Park/Wilber Palma|"Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of oac-21. Universal 43 bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). left flanking sequence: CACAAAAATCTTCAAAACGACTAGATCTTCAAGGC right flanking sequence: ATCCGGGCTCTCGCTATTCTAGTTGTTCTTGGCTTTC inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: GACTAGATCTTCAAGGCATC Method Reference: G3 (Bethesda).2018 Nov 6;8(11):3607-3616"
Proper citation: RRID:WB-STRAIN:WBStrain00055279 Copy
http://www.wormbase.org/db/get?name=WBStrain00055312
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: syIs794; syIs337.
Notes: Made_by: Mark Zhang/Stephanie Nava|"syIs794 [F47D2.11::NLS::GAL4(sk)::VP64::let-858 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)]. cGAL driver for ASJ neurons. syIs337 [15xUAS::(delta)pes-10::GFP::let-858 3'UTR + ttx-3p::RFP + 1kb DNA ladder(NEB)] is a GFP cGAL effector."
Proper citation: RRID:WB-STRAIN:WBStrain00055312 Copy
http://www.wormbase.org/db/get?name=WBStrain00055274
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: syIs704; syIs300.
Notes: Made_by: Stephanie Nava/Shahla Gharib|"syIs704 [pdf-1p::NLS::cGAL(DBD)::gp41-1-N-intein::let-858 3'UTR + mbr-1p::NLS::gp41-1-C-intein::cGAL(AD)::let-858 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)]. Split cGAL driver for AIM neurons. syIs300 [15xUAS::(delta)pes-10::GFP::let-858 3'UTR + ttx-3p::RFP + 1kb DNA ladder(NEB)] is a GFP cGAL effector. Some worms do not express ttx-3p::RFP marker, but will consistently produce worms with the transgenic marker in next generation."
Proper citation: RRID:WB-STRAIN:WBStrain00055274 Copy
http://www.wormbase.org/db/get?name=WBStrain00055276
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: syIs707; syIs300.
Notes: Made_by: Stephanie Nava/Shahla Gharib|"syIs707 [osm-10p::NLS::cGAL(DBD)::gp41-1-N-intein::let-858 3'UTR, srb-6p::NLS::gp41-1-C-intein::cGAL(AD)::let-858 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)] split cGAL driver for PHA and PHB neurons. syIs300 [15xUAS::(delta)pes-10::GFP::let-858 3'UTR + ttx-3p::RFP + 1kb DNA ladder(NEB)] is a GFP cGAL effector. Some worms do not express ttx-3p::RFP marker, but will consistently produce worms with the transgenic marker in next generation."
Proper citation: RRID:WB-STRAIN:WBStrain00055276 Copy
http://www.wormbase.org/db/get?name=WBStrain00055275
Source Database: WormBase (WB)
Availability: unknown
Source References: PMID:38096407
Synonyms: syIs705; syIs300.
Notes: Made_by: Stephanie Nava/Shahla Gharib|"syIs705 [gcy-32p::NLS::GAL4(sk)::VP64::let-858 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)]. cGAL driver for AQR, PQR, and URX neurons. syIs300 [15xUAS::(delta)pes-10::GFP::let-858 3'UTR + ttx-3p::RFP + 1kb DNA ladder(NEB)] is a GFP cGAL effector. Some worms do not express ttx-3p::RFP marker, but will consistently produce worms with the transgenic marker in next generation."
Proper citation: RRID:WB-STRAIN:WBStrain00055275 Copy
http://www.wormbase.org/db/get?name=WBStrain00055314
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: syIs798.
Notes: Made_by: Mark Zhang/Stephanie Nava|"syIs798 [srt-47p::NLS::GAL4(sk)::VP64::let-858 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)]. cGAL driver for AWC-ON neuron."
Proper citation: RRID:WB-STRAIN:WBStrain00055314 Copy
http://www.wormbase.org/db/get?name=WBStrain00055281
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: syIs743; syIs300.
Notes: Made_by: Stephanie Nava/Shahla Gharib|"syIs743 [pks-1p::NLS::GAL4(sk)::VP64::let-858 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)] cGAL driver for CAN neurons. syIs300 [15xUAS::(delta)pes-10::GFP::let-858 3'UTR + ttx-3p::RFP + 1kb DNA ladder(NEB)] is a GFP cGAL effector. Some worms do not express ttx-3p::RFP marker, but will consistently produce worms with the transgenic marker in next generation."
Proper citation: RRID:WB-STRAIN:WBStrain00055281 Copy
http://www.wormbase.org/db/get?name=WBStrain00055266
Source Database: WormBase (WB)
Availability: unknown
Source References: PMID:38096407
Synonyms: syIs680; syIs300.
Notes: Made_by: Stephanie Nava/Shahla Gharib|"syIs680 [gcy-5p::NLS::GAL4(sk)::VP64::let-858 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)]. cGAL driver for ASEL neuron. syIs300 [15xUAS::(delta)pes-10::GFP::let-858 3'UTR + ttx-3p::RFP + 1kb DNA ladder(NEB)] is a GFP cGAL effector. Some worms do not express ttx-3p::RFP marker, but will consistently produce worms with the transgenic marker in next generation."
Proper citation: RRID:WB-STRAIN:WBStrain00055266 Copy
http://www.wormbase.org/db/get?name=WBStrain00055302
Source Database: WormBase (WB)
Affected Genes: WBGene00005903(srx-12)
Genomic Alteration: WBGene00005903(srx-12)
Availability: unknown
Source References: EMPTY
Synonyms: srx-12(sy1746) IV.
Notes: Made_by: Heenam Park|"Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of srx-12. Universal 43 bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). left flanking sequence: CCATTGCAAATTTTGGGGTTCTATTTGTATTCTGC right flanking sequence: ACATGGGTCACGCCGACCACTATTATgtaggtttttg inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: TTCTATTTGTATTCTGCACA Method Reference: G3 (Bethesda).2018 Nov 6;8(11):3607-3616"
Proper citation: RRID:WB-STRAIN:WBStrain00055302 Copy
http://www.wormbase.org/db/get?name=WBStrain00055268
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: syIs690; syIs300
Notes: Made_by: Chun-Hao Chen/Stephanie Nava|"syIs690 [tph-1p::NLS::cGAL(DBD)::gp41-1-N-intein::let-858 3'UTR +pdf-1p::NLS::gp41-1-C-intein::cGAL(AD)::let-858 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)]. [NOTE: Pick animals with RFP+ coelomocytes to maintain. Array appears to be lost or silenced in some animals.] Split cGAL driver for NSM neuron. syIs300 [15xUAS::(delta)pes-10::GFP::let-858 3'UTR + ttx-3p::RFP + 1kb DNA ladder(NEB)] is a GFP cGAL effector. Some worms do not express ttx-3p::RFP marker, but will consistently produce worms with the transgenic marker in next generation."
Proper citation: RRID:WB-STRAIN:WBStrain00055268 Copy
http://www.wormbase.org/db/get?name=WBStrain00055301
Source Database: WormBase (WB)
Affected Genes: WBGene00000683(col-109)
Genomic Alteration: WBGene00000683(col-109)
Availability: unknown
Source References: EMPTY
Synonyms: col-109(sy1744) IV.
Notes: Made_by: Heenam Park|"Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of col-109. Universal 43 bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). left flanking sequence: gatcaccttttcccccattcgttttttccagTC right flanking sequence: GACCGCCAGAGATATCATGTCTGAAATCAGTCACAAG inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: TGATATCTCTGGCGGTCGAC Method Reference: G3 (Bethesda).2018 Nov 6;8(11):3607-3616"
Proper citation: RRID:WB-STRAIN:WBStrain00055301 Copy
http://www.wormbase.org/db/get?name=WBStrain00055304
Source Database: WormBase (WB)
Affected Genes: WBGene00005205(srg-48)
Genomic Alteration: WBGene00005205(srg-48)
Availability: unknown
Source References: EMPTY
Synonyms: srg-48(sy1750) IV.
Notes: Made_by: Heenam Park|"Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of srg-48. Universal 43 bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). left flanking sequence: CATTCTCCAGAACTATTCAATTTCTTCATGTTTTGCG right flanking sequence: GGCTGGCATTTCTTCATCTTCAGTCATGTAGTTC inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: TTTCTTCATGTTTTGCGGGC Method Reference: G3 (Bethesda).2018 Nov 6;8(11):3607-3616"
Proper citation: RRID:WB-STRAIN:WBStrain00055304 Copy
http://www.wormbase.org/db/get?name=WBStrain00055306
Source Database: WormBase (WB)
Affected Genes: WBGene00008570(kcnl-2)
Genomic Alteration: WBGene00008570(kcnl-2)
Availability: unknown
Source References: EMPTY
Synonyms: kcnl-2(sy1754) I.
Notes: Made_by: Heenam Park|"Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of kcnl-2. Universal 43 bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette) into very 1st exon of the gene. left flanking sequence: GTGATGTAAACGAAATTCCAAAAACGAATGGAGG right flanking sequence: AGGACATCCAATTGTTAGAAGAAAAAGTGGAATG inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: CCAAAAACGAATGGAGGTCC Method Reference: G3 (Bethesda).2018 Nov 6;8(11):3607-3616"
Proper citation: RRID:WB-STRAIN:WBStrain00055306 Copy
http://www.wormbase.org/db/get?name=WBStrain00055305
Source Database: WormBase (WB)
Affected Genes: WBGene00005739(srv-28)
Genomic Alteration: WBGene00005739(srv-28)
Availability: unknown
Source References: EMPTY
Synonyms: srv-28(sy1752) IV.
Notes: Made_by: Heenam Park|"Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of srv-28. Universal 43 bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). left flanking sequence: GTATACTATGGAATGTCGATTTTAAGTCTTCCCTTATA right flanking sequence: CTTTGGTGTTCTCATTTGTTTGTTGAGATTGAG inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: TTAAGTCTTCCCTTATACTT Method Reference: G3 (Bethesda).2018 Nov 6;8(11):3607-3616"
Proper citation: RRID:WB-STRAIN:WBStrain00055305 Copy
http://www.wormbase.org/db/get?name=WBStrain00055270
Source Database: WormBase (WB)
Affected Genes: WBGene00017483(lgc-22)
Genomic Alteration: WBGene00017483(lgc-22)
Availability: unknown
Source References: EMPTY
Synonyms: lgc-22(sy1478) IV.
Notes: Made_by: Heenam Park & Mandy Tan|"Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of lgc-22. Universal 43 bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). left flanking sequence: GGCTTCTCATTGCCACGTCACTGGCCGCCACGTTA right flanking sequence: GCGTGGGCGGCGGGCACCGCCGGCGGCTGCGAGGAC inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: CACTGGCCGCCACGTTAGCG Method Reference: G3 (Bethesda).2018 Nov 6;8(11):3607-3616"
Proper citation: RRID:WB-STRAIN:WBStrain00055270 Copy
http://www.wormbase.org/db/get?name=WBStrain00055256
Source Database: WormBase (WB)
Availability: unknown
Source References: PMID:38096407
Synonyms: syIs654.
Notes: Made_by: Jun Young Oh/Stephanie Nava|"syIs654 [15xUAS:VSFP-Butterfly-1-2::let-858 3'UTR + ttx-3p::RFP + 1kb DNA ladder (NEB)]. Voltage-sensitive fluorescent protein cGAL effector."
Proper citation: RRID:WB-STRAIN:WBStrain00055256 Copy
http://www.wormbase.org/db/get?name=WBStrain00055255
Source Database: WormBase (WB)
Availability: unknown
Source References: PMID:38096407
Synonyms: syIs640.
Notes: Made_by: Chun-Hao Chen/Stephanie Nava|"syIs640 [15xUAS::fem-3::mCherry::let-858 3'UTR + ttx-3p:RFP + 1kb DNA ladder (NEB)] cGAL effector to induce masculinization"
Proper citation: RRID:WB-STRAIN:WBStrain00055255 Copy
http://www.wormbase.org/db/get?name=WBStrain00055257
Source Database: WormBase (WB)
Availability: unknown
Source References: PMID:38096407
Synonyms: syIs657.
Notes: Made_by: Jun Young Oh/Stephanie Nava|"syIs657 [15xUAS::act-2::Venus::let-858 3'UTR + ttx-3p::RFP + 1kb DNA ladder (NEB)]. cGAL effector for localiziation of actin filament and cell cortex"
Proper citation: RRID:WB-STRAIN:WBStrain00055257 Copy
http://www.wormbase.org/db/get?name=WBStrain00055261
Source Database: WormBase (WB)
Affected Genes: WBGene00010172(oac-36)
Genomic Alteration: WBGene00010172(oac-36)
Availability: unknown
Source References: EMPTY
Synonyms: oac-36(sy1411) I.
Notes: Made_by: Heenam Park & Mandy Tan|"Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of oac-36. Universal 43 bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). left flanking sequence: CTAATGTGCATGTTGCTCAAGCGTGCCGAGACCAAGC right flanking sequence: CATTTTTCACAGTGGTATGCACATTTTACACGAGG inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: TACCACTGTGAAAAATGGCT Method Reference: G3 (Bethesda).2018 Nov 6;8(11):3607-3616"
Proper citation: RRID:WB-STRAIN:WBStrain00055261 Copy
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