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THIS RESOURCE IS NO LONGER IN SERVICE. Documented on November 14,2023.Automated preparation system that isolates and prepares single cell templates for analysis. It can analyze mRNA and DNA sequencing, epigenetics or miRNA expression. It can extract, reverse transcribe, amplify, and analyze gene expression.
Proper citation: Fluidigm: C1 Single-Cell Automated Preparation System (RRID:SCR_018578) Copy
https://www.marshallscientific.com/Fisher-Scientific-PowerGen-125-Homogenizer-p/fs-pg125.htm
Homogenizer with speeds from 8,000 to 30,000 RPM for volumes of up to 100ml. Homogenizer for production of dispersions and emulsions, plant tissues, human tissues, animal tissues, resins, and pigments.
Proper citation: Thermo Fisher: n125 Homogenizer (RRID:SCR_018576) Copy
https://www.gilson.com/verity-271-hplc-system.html
HPLC liquid handler with midsize bed capacity and fraction collector. It has maximum flow rates up to 200 mL per min, injection loop volumes of 2 uL to 25 mL, and max sample mass of 15 grams per injection.
Proper citation: Gilson: GX-271 HPLC System (RRID:SCR_018579) Copy
https://www.illumina.com/documents/products/datasheets/datasheet_cbot.pdf
Genetic amplifier that uses amplification to create single molecule DNA templates and dispense reagents. It also controls reaction times, flow rates, and temperatures.
Proper citation: Illumina: cBot Genetic Amplifier (RRID:SCR_018580) Copy
https://www.kentscientific.com/products/coda-high-throughput-system/
CODA mouse rat tail-cuff system was designed to allow accurate blood pressure measurement in mice and rats. Blood pressure is measured in the tail of the mouse or rat using Volume Pressure Recording (VPR) sensor technology. Using the included software allows you to continuously view data in real-time.
Proper citation: Kent Scientific: Coda Blood Pressure System (RRID:SCR_018585) Copy
https://www.agilent.com/cs/library/brochures/5990-5596EN.pdf
Agilent 3100 OFFGEL Fractionator performs isoelectric focusing of proteins or peptides in immobilized pH gradient (IPG) gel strips.
Proper citation: Agilent: 3100 OFFGEL Fractionator (RRID:SCR_018433) Copy
https://mobile.labwrench.com/equipment/8044/applied-biosystems/voyager-de-pro
Benchtop MALDI TOF mass spectrometer that has linear and reflector analyzer. It is used to analyze organic and inorganic compounds, protein identification, synthetic peptide validation, and polymer analysis.
Proper citation: Thermo Fisher: Applied Biosystems: ABI Voyager DE Pro MALDI-Mass Spectrometer (RRID:SCR_018432) Copy
https://www.thelabworldgroup.com/mettler-toledo-pb303-precision-balance
Miligram balance that is used for weighing, tarring, percent weighing, and dynamic weighing for unstable samples. Has maximum capacity of 310 grams. Lab balance utilizes mono block weighing technology with permanent shock protection and built in overload protection as well as automatic calibration.
Proper citation: Mettler: PB303-S Milligram Balance (RRID:SCR_018592) Copy
Invitrogen benchtop automated cell counter. Can perform cell counts, viability calculations, hemocytometry, fluorescent protein expression, and measure fluorescence capabilities. There are also reusable slides, fluorescence capabilities, and brightfield fluorescence channels.
Proper citation: Thermo Fisher: Life Technologies: Countess Automated Cell Counter (RRID:SCR_018591) Copy
Florida State University is a public research university in Tallahassee, Florida, United States. It is a senior member of the State University System of Florida and a preeminent university in the state. Chartered in 1851, it is located on Florida's oldest continuous site of higher education.
Proper citation: Florida State University; Florida; USA (RRID:SCR_011228) Copy
http://tools.thermofisher.com/content/sfs/manuals/nd-1000-v3.8-users-manual-8%205x11.pdf
Spectrophotometer for measurement and analysis of 1 ul samples with high accuracy and reproducibility. Full spectrum from 220nm to 750nm spectrophotometer utilizes patented sample retention technology that employs surface tension alone to hold sample in place. No need for cuvettes. Has capability to measure highly concentrated samples without dilution.
Proper citation: Thermo Fisher: Nanodrop 1000 Spectrophotometer (RRID:SCR_016517) Copy
http://tools.thermofisher.com/content/sfs/manuals/cms_040970.pdf
Automated PCR instrument for automated amplification of nucleic acids with Polymerase Chain Reaction. It has reaction volumes of up to 50 uL and sample temperature range of 4 to 99.9 C.
Proper citation: Thermo Fisher: Applied Biosystems: GeneAmp 9700 PCR Thermocycler System (RRID:SCR_018436) Copy
Center that imports, archives, maintains, and distributes mutant mouse alleles as live mice, frozen germplasm, stem cells, and molecular vectors for use in biomedical research. The MMRRC Davis receives transgenics, knockouts, and other kinds of mutant mouse lines at no cost to the donor, and after re-derivation and cryopreservation, distributes breeding stock, germplasm, cells, or tissues of genetically-defined and pathogen-free mice for a small fee to requesting investigators.
Proper citation: University of California at Davis Mutant Mouse Resource and Research Center (RRID:SCR_016448) Copy
https://www.rankinbiomed.com/product/refurbished-american-optical-860-sliding-micrtome/
THIS REOURCE IS NO LONGER IN SERVICE. Documented on September 29,2023. Sliding microtome used for cutting hard objects such as bone, wood, frozen celloidin, and paraffin preparations. Cutting thickness is adjustable from 1 to 40 microns.
Proper citation: American Optical: 860 Sliding Microtome (RRID:SCR_018434) Copy
https://timothyspringer.org/files/tas/files/biacore3000-instrument.pdf
Biacore 3000 processing unit is an established, label-free system for detailed studies of biomolecular interactions. The system delivers comprehensive characterization of the interaction, answers questions about the rate constants, affinity, specificity, and determines the active concentration of components. The ability to recover and transfer interaction partners directly to MALDI targets for identification and further characterization makes the system highly applicable to fast identification of unknown interactants.
Proper citation: GE: Biacore 3000 Real Time Biomolecular Interaction Analyzer (RRID:SCR_018044) Copy
https://www.ttuhsc.edu/pharmacy/research/documents/Amnis-Imagestream-Manual.pdf
Bench top multispectral imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. It combines per cell information with statistical significance of sample sizes of standard flow cytometry.
Proper citation: Luminex: Amnis ImageStreamX Mk II System (RRID:SCR_018589) Copy
https://coreimmunology.ucsf.edu/services
Provides immunology assays, expert consultation, and education to UCSF and global research community. CIL specializes in developing immunology research tools, and initiating and stimulating innovative research projects to address emerging questions in immunologic diseases including HIV pathogenesis.Services include:Isolation of PBMC for staining, cell sorting, or viable cryopreservation;Isolation of mucosal mononuclear cells (MMC) from mucosal tissue biopsies;Immunomagnetic bead isolation of untouched cell populations for functional studies;Immunophenotyping of whole blood, PBMC, MMC and cerebrospinal fluid for: NK, B and T cells percentages, and their subsets, including maturation, trafficking, senescence, activation, in vivo proliferation and chemokine receptor expression;Flow cytometric functional assays, including NK cell function, antigen-specific CD4+ and CD8+ T cell cytokine expression, degranulation and proliferation;Evaluation of T cell signaling by phosflow using monoclonal antibodies specific for phosphorylated protein;Training and Support;Flow Cytometry Training Course;Three-hour lecture and lab course designed to cover basics of flow cytometry including:Fluorescence emission and detection,Fluidics,Operation,Panel design,Controls.
Proper citation: University of California at San Francisco Immunology Lab Core Facility (RRID:SCR_017906) Copy
Core provides services, access to instruments, and training to facilitate production of animal tissue samples for morphological and molecular analyses. Supports multiplex immunofluorescence staining, RNA in situ hybridization (ISH), and imaging analysis. Services include tissue preparation, processing, and sectioning, as well as histochemistry and immunohistochemistry of fresh, frozen, and fixed animal tissues, can optimize and validate commercial or proprietary antibodies for chromogenic or fluorescence-based immunohistochemistry including seven-color multiplexing.Provides access to database listing antibodies that have been tested in lab to date and are currently in stock.Offers basic imaging resources and digital whole-slide scanning of stained tissue sections, which allows researchers to view their slides online at their convenience.Researchers can also rent our self-service instruments. These include embedding station, microtome, cryostat, and vibratome. Provides access to brightfield and fluorescence upright and dissecting microscopes,laser capture microdissection scope, and high-throughput multispectral imaging system.To reserve time on instrument, faculty, staff, and investigators must be registered in iLab.
Proper citation: New York University School of Medicine Langone Health Experimental Pathology Research Laboratory Core Facility (RRID:SCR_017928) Copy
https://www.unmc.edu/vcr/cores/vcr-cores/nanoimaging/index.html
Provides service and expert advice to researches in their projects related to nanoimaging studies of molecular systems at single molecule and single cell levels utilizing Atomic Force Microscope, combination of Atomic Force Microscope with Fluorescence Optical Microscope, combination of Atomic Force Microscope with Raman Spectroscopy.
Proper citation: University of Nebraska Medical Center Nanoimaging Core Facility (RRID:SCR_018828) Copy
http://www.med.nyu.edu/ocs/cytometry-and-cell-sorting-core
Facility provides cytometry and cell sorting services. Services include immunophenotyping, cell cycle analysis, and intracellular fluorophore detection. Services can be tailored to research needs, certified cytometrists are available to help plan experiments. Offers online and hands-on training in use of cell analyzers and analysis software.
Proper citation: New York University School of Medicine Langone Cytometry and Cell Sorting Core Facility (RRID:SCR_018827) Copy
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