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https://edspace.american.edu/openbehavior/project/nerd/
Portal provides open source neural recording device for wireless transmission of local field potential data in freely behaving animals. Used for electrophysiological recording of local field potentials in freely behaving animals.
Proper citation: NeRD (RRID:SCR_021582) Copy
https://edspace.american.edu/openbehavior/project/ninscope/
Portal provides miniscope for fluorescence imaging and optogenetic stimulation. Used for monitoring of neural signals during unrestrained behavior.
Proper citation: NINscope (RRID:SCR_021577) Copy
https://edspace.american.edu/openbehavior/project/3d-printed-headcap-microdrive/
Portal provides novel head cap and microdrive design for chronic multi electrode recordings in rats through use of 3D printing technology. Head cap and microdrive designs can be fabricated at little cost using consumer grade 3D printers.
Proper citation: 3D Printed Headcap and Microdrive (RRID:SCR_021595) Copy
https://edspace.american.edu/openbehavior/project/multicam-headmount/
Portal provides system of two multi camera head mounts for mice in order to record physiological changes in certain behaviors. System used for video tracking of rodents behavior.
Proper citation: A Head Mounted Multi Camera System for Freely Moving Mice project (RRID:SCR_021591) Copy
https://edspace.american.edu/openbehavior/project/microwave-based-homecage-motion-detector/
Portal provides radar based motion detector for home cage activity monitoring in mice. System is used for motion detection without visual contact to animal and can be used with various cage types. Features custom printed circuit board and motion detector shield for Arduino, which saves raw activity and timestamped data in CSV files onto SD card.Developed by Max Planck Institute of Psychiatry scientists. Authors also provide Python script for data analysis and generation of actograms.
Proper citation: Microwave based Homecage Motion Detector project (RRID:SCR_021590) Copy
Portal provides open source lickometer that utilizes readily available or customizable components such as PC sound card and 3D printed drinking bottle holder. System consists of apparatus itself and open source software to collect and analyze data. Recording of raw data is handled by Audacity, open source audio program.
Proper citation: An Opensource lickometer and microstructure analysis program (RRID:SCR_021589) Copy
https://edspace.american.edu/openbehavior/project/pried/
Portal provides open Source 3-D Printed Modular micro drive system for acute primate neurophysiology.
Proper citation: PRiED (RRID:SCR_021586) Copy
https://edspace.american.edu/openbehavior/project/maple/
Portal related to measurement of fly social networks and behavior. Provides Drosophila handing robot that boasts modular design, allowing platform to both automate diverse phenotyping assays and aid with lab chores (e.g., collecting virgin female flies). MAPLE permits small part manipulator, USB digital camera, and fly manipulator to work simultaneously over platform of flies.
Proper citation: Modular Automated Platform for Large Scale Experiments project (RRID:SCR_021557) Copy
https://edspace.american.edu/openbehavior/project/arcontrol/
Portal related to delivering stimuli and monitoring responses. Provided behavioral platform was developed by scientists from Huazhong University of Science and Technology, Wuhan, China. System uses Arduino UNO board and simple drive circuit along with stand-along GUI application. Experimental data is automatically recorded with built-in data acquisition function and entire behavioral schedule is stored within Arduino chip.
Proper citation: ArControl project (RRID:SCR_021556) Copy
https://edspace.american.edu/openbehavior/project/snackclock/
Project related to feeding behavior.Provides device for dispensing pre-measured quantities of food at regular intervals throughout day. Device uses 24 hour clock movement to rotate dispenser wheel one revolution per day.
Proper citation: SnackClock (RRID:SCR_021554) Copy
https://edspace.american.edu/openbehavior/project/automated-home-cage-functional-imaging/
Portal provides automated system for mesoscopic functional imaging that allows subjects to self initiate head fixation and imaging within home cage. Developed by University of British Columbia scientists. System for automated head fixation within mouse home cages.
Proper citation: Automated Home-Cage Functional Imaging project (RRID:SCR_021567) Copy
https://edspace.american.edu/openbehavior/project/acrobat/
Project related to studying motor behavior. University of Washington in Seattle scientists have developed and published their device for training rats to perform modified center out task.
Proper citation: ACRoBaT (RRID:SCR_021546) Copy
https://edspace.american.edu/openbehavior/project/multichromvisstim/
Project related to visual neuroscience.Provides spatial visual stimulator with arbitrary spectrum of light for visual neuroscientists.Open source, low cost visual stimulator which can be customized with up to 6 chromatic channels. Spectrum of light can be arbitrary and customizable to be adapted to different animal models based on their visual spectrum. Device details, including parts list and information for custom python library for generating visual stimuli (QDSpy), can be found in eLife publication.
Proper citation: Visual stimulator with customizable light spectra (RRID:SCR_021545) Copy
https://www.lri.se/pdf/aperio/LRI_ScanScope_FL.pdf
Aperio slide scanner for fluorescent images. Used for whole slide scanning of slides stained with multiple fluorochromes. With quad multi-bandpass filter set, up to four color channels can be acquired; users can add other filters for greater flexibility. Advanced illumination, autoexposure, and autofocus capabilities eliminate need for trial-and-error scanning, reducing problem of photobleaching.
Proper citation: Aperio: ScanScope FL System (RRID:SCR_022191) Copy
https://autom8.com/shop/brain-slice-tissue-chambers/brain-slice-incubators/brain-slice-keeper-4/
Slice keeper holds four sets of brain slices and features container with lid and integrated air stone for bubbling carbogen into ACSF, needle valve for adjusting gas flow included, and curved bottom designed to keep bubbles away from slices while promoting fresh solution flow over slices.
Proper citation: Automate Scientific: Brain Slice Keeper 4 (RRID:SCR_018943) Copy
Seahorse XF Imaging and Normalization System acquires brightfield and fluorescence images, calculates cell number in each well and transfers images and cell counts into Wave 2.6 software.
Proper citation: Agilent: Seahorse XF Imaging and Normalization System (RRID:SCR_019543) Copy
TPS-compact Turbo Pumping System is used for pump operation, control, and recording of system parameters.
Proper citation: Agilent: TPS-compact Turbo Pumping System (RRID:SCR_019546) Copy
Remote control unit drives pump in real time and adapts speed and power as required by gas flow, gas types, pressures, and other factors such as cooling.
Proper citation: Agilent: TwisTorr 305 FS Remote Controller (RRID:SCR_019558) Copy
Luminescent assays are sensitive and quantitative tools commonly used for a variety of purposes in biomedical and pharmaceutical research. Quantitation of ATP can be used to detect and measure cellular growth, as well as bacterial contamination. Luminescent genetic reporting assays are widely used to study gene expression and cellular responses to external stimuli in Prokaryotic and Eukaryotic organisms. Dual-reporter assays use two independent reporter systems simultaneously to improve experimental accuracy. One reporter is used to measure the response resulting from the experimental conditions and is often referred to as the “experimental� reporter. The second reporter is designed not to respond to the experimental conditions, acting as an internal control from which data generated by the experimental reporter can be normalized. Normalization of the data serves to compensate for variability caused by differences in transfection efficiency, cell viability, cell lysis, and pipetting. Promega's Dual-Luciferase System uses the activities of luminescent proteins (luciferases) from the firefly (Photinus pyralis) beetle and the sea pansy (Renilla renformis) to serve as an experimental and a control reporter respectively (See Figure 1). The Synergy HT Multi-Detection Reader (BioTek Instruments, Winooski, VT) is a robotic compatible microplate reader that can measure absorbance, fluorescence, and luminescence in all plate formats up to 384-well plates with performance in all three detection modes, that has recently been optimized for luminescence measurements (Figure 2). The Synergy HT utilizes a dual optics design that has both a monochromator/xenon flash system with a silicone diode detector for absorbance and a tungsten halogen lamp with blocking interference filters and a photomultiplier tube (PMT) detector for fluorescence. Reagent addition is accomplished by an optional external injector module, which controls two independent injector syringes. Each syringe is connected to a separate injector tip. Emitted luminescence is captured using either the top or bottom probes and measured using the Synergy HT's low noise PMT operated in photon integration mode. Here we describe the use of a Synergy HT Multi-Detection Microplate Reader (BioTek Instruments) to perform dual-luciferase measurements with purified recombinant enzymes.
Proper citation: Agilent: BioTek: Synergy HT Plate Reader (RRID:SCR_020536) Copy
TwisTorr 74 FS AG Rack Controller adapts speed and power as required by gas flow, gas type, pressures, and other factors such as cooling.
Proper citation: Agilent: TwisTorr 74 FS AG Rack Controller (RRID:SCR_019554) Copy
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