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https://med.nyu.edu/research/scientific-cores-shared-resources/proteomics-laboratory

Core offers specialized expertise for analysis of proteins and peptides using mass spectrometry. Develops new methods and customized approaches for proteomic analysis and suggests experimental strategies and sample preparation prior to mass spectrometry analysis. Services include:comprehensive protein identification ,analysis of affinity purified complexes,characterizing protein post-translational modifications,de novo sequencing,label and label-free quantitation ,multiplexed quantitation global phosphorylation and ubiquitin analysis,analysis of laser-capture microdissected formalin-fixed paraffin-embedded tissue,secretome analysis,crosslinking analysis,disulfide mapping.

Proper citation: New York University School of Medicine Langone Health Proteomics Laboratory Core Facility (RRID:SCR_017926) Copy   

•   Source: SciCrunch Registry

https://www.qut.edu.au/institute-for-future-environments/facilities/central-analytical-research-facility/our-laboratories/proteomics-and-small-molecule-mass-spectrometry-laboratory

Core provides liquid chromatography and gas chromatography mass spectrometry instrumentation for selective identification and reproducible quantification of trace-level biomolecules in complex samples. Services include qualitative, quantitative and structural analysis of proteins, lipids, metabolites, pesticides, pharmaceuticals and volatile organic compounds. Tests can be performed as long as molecules of interest are amenable to ionisation technique employed in source of mass spectrometer. Depending on instrument type, samples may be solid, liquid or gas. Mass spectrometry platforms include Liquid Chromatography Mass Spectrometry (LC-MS) and Gas Chromatography Mass Spectrometry (GC-MS). Analysis include volatile organic compounds (odour analysis),pharmacokinetics (bioavailability, bioefficacy),drug development (determining structures of drugs and metabolites),clinical testing (biomarkers discovery and endogenous compounds),genomics (oligonucleotide sequencing) epigenetics (global DNA methylation analysis),environmental research (testing water, soil, food and air quality).

Proper citation: Queensland University of Technology Central Analytical Research Facility (CARF) Proteomics and Small Molecule Mass Spectrometry Core Facility (RRID:SCR_017933) Copy   

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http://ccb.ku.edu/

Core provides computational resources and expertise to enhance productivity of researchers studying infectious diseases. Assists with virtual screening, protein-small molecule docking, binding site prediction, protein modeling and design, prediction of protein stability changes upon mutation, fragment based probe design, as well as preparation of presentation graphics.Specializes in initial hit identification of non-traditional drug targets such as protein-protein or protein-RNA interfaces by offering high-throughput virtual screening via pocket optimization with exemplar screening at protein-protein interfaces and hotspot pharmacophore mimicry of protein-RNA interactions.CCB works in collaboration with Molecular Graphics and Modeling Laboratory.

Proper citation: Kansas University at Lawrence Computational Chemical Biology Core Facility (RRID:SCR_017890) Copy   

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http://rppc.mccormick.northwestern.edu/

Core provides quality controlled recombinant proteins. rPPC operates based on the two service models: Training model where Northwestern researchers use specialized bioreactor systems and participate in hands-on-training activities and Production model where staff carry out expression (mg to gm scale) and purification of recombinant or synthetic biologics, including potential therapeutic proteins and peptides, among others. Main focus of rPPC is to be user-facility;facility has parallel bioreactor systems for multiplexed lab-scale cultivation of microbial, insect, and mammalian cells. rPPC also serves as a production facility, providing low-cost recombinant biologics for researchers at Northwestern University.Services include: TRANSFECTION/TRANSFORMATION, ANALYTICAL (SMALL SCALE) EXPRESSION IN E.COLI AND MAMMALIAN CELLS,PROTEIN EXPRESSION IN E. COLI (LARGE SCALE),PROTEIN EXPRESSION IN MAMMALIAN CELL SYSTEM (LARGE SCALE),PROTEIN EXPRESSION IN INSECT CELL SYSTEM,GENERATION OF MOUSE HYBRIDOMA, PRODUCING MONOCLONAL ANTIBODIES, per one fusion,RESCUING AND CULTIVATING EUKARYOTIC CELLS,DOWNSTREAM PROCESSING OF GROWN CULTURE (BEFORE PROTEIN PURIFICATION),RECOMBINANT PROTEIN PURIFICATION,TAG CLEAVAGE WITH TEV. PROTEASE,LARGE SCALE mAb PRODUCTION ,DNA PLASMID PROPAGATION AND PURIFICATION,SDS-PAGE ANALYSIS,WESTERN BLOT ANALYSIS, INSTRUMENT TIME SHARING TECHNICAL/INSTRUMENT ASSISTANCE TIME,PROTEIN RECOVERING FROM INCLUSION BODIES.

Proper citation: Northwestern University Recombinant Protein Production Core Facility (RRID:SCR_017872) Copy   

•   Source: SciCrunch Registry

http://www.med.unc.edu/csb/pep

Core specializes in production of pure, functional proteins for structural, biophysical, and biochemical studies. Facility offers three categories of service:Protein Expression,Protein Purification,Scientific Consultation, Mentoring, and Training; Offers Isotope labeled proteins for NMR;High production scales for immunizations, drug discovery, structural biology;Endotoxin-free protein production;Stable cell line generation;Expert baculovirus expression;Custom packages to efficiently suit your needs.

Proper citation: North Carolina University at Chapel Hill School of Medicine Protein Expression and Purification Core Facility (RRID:SCR_017843) Copy   

•   Source: SciCrunch Registry

https://my.ilabsolutions.com/service_center/show_external/4003

Core specializes in cell, protein, and small molecules analysis as well as cell culture techniques. Services include:2-D gel electrophoresis, 2-D DIGE, LC-MS/MS, HPLC, flow cytometry, fluorescence-activated cell sorting (FACS), cell and tissue culture, and immortalization of cell lines. Our staff works closely with investigators to help design, perform, and analyze experiments.Offers training and assistance in flow cytometry, tissue culture, and operation many of our walk-up instruments.Instruments:Cell Sorter: FACS Aria III, BD Biosciences;Flow Cytometers, analyzers:C6, Accuri/BD Biosciences;Novocyte 3000, ACEA Biosciences;software for analysis: FSC Express, DeNovo software;LC-MS/MS: 6460 Triple Quadrupole, Agilent;Typhoon Trio Scanner, GE Lifesciences;Blood Analyzer: Hemavet 950, Drew Scientific.Plate Readers:;Victor Nivo 5F, Perkin Elmer;Luminometer: Centro XS, Berthold.Services:Cell Sorting (FACS);2-D gel electrophoresis/2D-DIGE;LC-MS/MS analysis of compounds; Cell immortilization.

Proper citation: Nemours/A.I.duPont Hospital for Children Cell Science Core Facility (RRID:SCR_017854) Copy   

•   Source: SciCrunch Registry

https://www.waksman.rutgers.edu/fermentation

Provides fermentation services. Produces range of bulk biologics including antimicrobials, cosmetic substrates, flavors/fragrances, biopesticides/bioherbicides and plasmid derived proteins, enzymes, growth factors and diagnostics. Exceptions for pathogenic or opportunistic organisms and mammalian and insect cell lines. Includes Material Preparation Laboratory,Fermentation In-Process/Computer Control,Product Recovery and Analytical Services.

Proper citation: Rutgers Waksman Institute of Microbiology Cell and Cell Products Fermentation Core Facility (RRID:SCR_018676) Copy   

•   Source: SciCrunch Registry

http://cobre.pbrc.edu/cores/genomics/

Provides services which include Sanger and next-generation DNA sequencing,DNA fragment analysis,qualitative and quantitative analysis of DNA, protein, and RNA samples, quantitative PCR, microarray RNA labeling, hybridization, and scanning robotics,bioinformatics.Individual and small group training and consultation services are offered for sequence analysis, real-time PCR, next-generation sequencing and microarray analysis.

Proper citation: Louisiana State University Pennington Biomedical Research Center Genomics Core Facility (RRID:SCR_018675) Copy   

•   Source: SciCrunch Registry

http://psf.cobre.ku.edu/cores/bnmrl/about

NMR Laboratory maintains two high field NMR spectrometers in support of structural and dynamics studies of biomolecules. Its capabilities include determining high resolution structures, biological macromolecules, elucidation and structural mapping of protein-protein, protein-nucleic acid, protein-peptide, protein-drug interactions, and studies of dynamics of proteins and their complexes in solution. Laboratory staff provide advice,consultation, training,assistance and complete structure elucidation services.Staff is responsible for maintenance, upgrades, implementation of new NMR pulse sequences, and assisting local and remote users with technical problems.Equipped with Bruker Avance 800 MHz NMR instrument fitted with TCI cryoprobe and Bruker Avance III 600 MHz with variety of probes.

Proper citation: Kansas University Lawrence Biomolecular NMR Laboratory Core Facility (RRID:SCR_018671) Copy   

•   Source: SciCrunch Registry

http://cores.musc.edu/Core/MS

Core provides expertise, services, education, and instrumentation to enhance biomedical research through LC-MS/MS-based proteomics. Services are offered for protein identification; characterization of post-translational modifications; and quantitative proteomics to identify differentially expressed/degraded proteins, regulated sites of post-translational modification, protein-protein interactions, and protein targets of drugs identified in phenotypic screens. Analyses include sample preparation, LC-MS/MS, database searching, generation of reports, and assistance with data interpretation. Faculty and staff assist with experimental design and development/optimization of customized methodology for analysis of post-translationally modified peptides (e.g. phosphorylation and O-GlcNAc modification, N- and O-linked glycosylation, Cys modifications including S-glutathionylation, and glycation of Lys and Arg). Quantitative approaches including metabolic labeling (SILAC), isobaric tagging (iTRAQ/TMT), and label free proteomics (LFQ) are performed on Orbitrap Elite or Orbitrap Fusion Lumos Mass Spectrometers. Developes methodology to identify alterations in post-translational modifications that impact signal transduction, transcription, translation, and response to therapeutics with goal of enabling investigators to discover molecular mechanisms underlying disease progression and therapeutic response.

Proper citation: South Carolina Medical University Mass Spectrometry Core Facility (RRID:SCR_017959) Copy   

•   Source: SciCrunch Registry

https://vbrn.org/proteomics-services/

Provides central resource of mass spectrometry based proteomics technologies to identify, characterize and quantify target proteins in various biological and biomedical samples. Provides mass spectrometry expertise for analyzing proteins and peptides for proteomics studies, support for data analysis from proteomics measurements, training in proteomics methods, and experimental design.

Proper citation: Vermont University Proteomics Core Facility (RRID:SCR_018667) Copy   

•   Source: SciCrunch Registry

http://ncore.web.unc.edu/

Core provides physicochemical characterization of nanoscale entities. Offers characterization of several classes of nanomaterials:Polymer conjugates,Polymeric micelles,Liposomes,Nanogels,Polyion complexes of small drugs and biomacromolecules (proteins, DNA, and RNA),Inorganic/metal nanoparticles,Bio-derived nanoparticles such as exosomes with protein and nucleic acid cargo.

Proper citation: North Carolina University at Chapel Hill Nanomedicines Characterization Core Facility (RRID:SCR_017951) Copy   

•   Source: SciCrunch Registry

https://education.musc.edu/colleges/medicine/departments/pharmacology/redox-center/cores/analytical-redox-biology-core

Core provides analytical redox biochemistry methods and mentoring support for COBRE junior faculty with goal to advance their research endeavors, publications and fundability. Specific aims are:Provide ROS /RNS identification and quantification, Perform quantitative analysis of ROS/RNS. Provides expertise and technology for in depth biochemical analysis of thiol-centered enzyme activities and define protein:protein interactions.

Proper citation: South Carolina Medical University Analytical Redox Biology Core Facility (RRID:SCR_017955) Copy   

•   Source: SciCrunch Registry

https://github.com/Wssduer/GraphRBF

Software tool as protein-protein/nucleic acid interaction site prediction model built by enhanced graph neural networks and prioritized radial basis function neural networks. Protein-protein and protein-nucleic acid binding site prediction via interpretable hierarchical geometric deep learning.

Proper citation: GraphRBF (RRID:SCR_025652) Copy   

•   Source: SciCrunch Registry

http://pridb.gdcb.iastate.edu/RPISeq

Web application for RNA-protein interactions prediction.

Proper citation: RPISeq (RRID:SCR_027257) Copy   

•   Source: SciCrunch Registry

https://www.tgh.org/institutes-and-services/cancer-institute/precision-medicine-bio-repository

Procures high-quality biologic specimens along with coded clinical and demographic data across broad ethnic, racial, and social demographic of patients. All specimens are systematically collected and processed so that their multi-functional potential for recovering high-quality bioanalytes (including RNA, genomic DNA, protein, enzymes and metabolites) is conserved. All specimens are stored and protected in a database hub to connect researchers conducting variety of scientific projects across multiple medical specialties. Muilti-disciplinary biorepository with capacity for generating specialized bioanalytes.

Proper citation: Tampa General Hospital-University of South Florida Health Precision Medicine Biorepository (RRID:SCR_027501) Copy   

•   Source: SciCrunch Registry