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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pAW-CD8-NbVHH05-GFP Resource Report Resource Website |
RRID:DGRC_1562 | Drosophila | Ampicillin | Nanobody, pAW backbone, Nanobody expression in fly cells, GFP, CD8 | Drosophila Genomics Resource Center | 2026-09-05 03:58:48 | 0 | ||||
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pUAST-aC-EGFP Resource Report Resource Website |
RRID:DGRC_1009 | Drosophila | Ampicillin | PMID:11102367 | Epitope Tagged Vectors, UAS/GAL4 Vectors, Recombination and Gateway Vectors, General Fly Transformation Vectors, white[+], P-elements, UAS enhancer, hsp70 promoter, SV40 3'UTR, eGFP tag | Drosophila Genomics Resource Center | 2026-09-05 03:58:44 | 0 | |||
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pUAST-aC-V5/6xHis Resource Report Resource Website |
RRID:DGRC_1012 | Drosophila | Ampicillin | Epitope Tagged Vectors, UAS/GAL4 Vectors, Recombination and Gateway Vectors, General Fly Transformation Vectors, white[+], P-elements, UAS enhancer, hsp70 promoter, SV40 3'UTR, V5 tag, 6X His tag | Drosophila Genomics Resource Center | 2026-09-05 03:58:44 | 0 | ||||
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pUAST-aC-ECFP Resource Report Resource Website |
RRID:DGRC_1011 | Drosophila | Ampicillin | PMID:11532957 | Epitope Tagged Vectors, UAS/GAL4 Vectors, Recombination and Gateway Vectors, General Fly Transformation Vectors, white[+], P-elements, UAS enhancer, hsp70 promoter, SV40 3'UTR, eCFP tag | Drosophila Genomics Resource Center | 2026-09-05 03:58:44 | 0 | |||
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pUAST-aC-EYFP Resource Report Resource Website |
RRID:DGRC_1010 | Drosophila | Ampicillin | PMID:10585440 | Epitope Tagged Vectors, UAS/GAL4 Vectors, Recombination and Gateway Vectors, General Fly Transformation Vectors, white[+], P-elements, UAS enhancer, hsp70 promoter, SV40 3'UTR, eYFP tag | Drosophila Genomics Resource Center | 2026-09-05 03:58:44 | 0 | |||
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IP16479 Resource Report Resource Website |
RRID:DGRC_1604016 | Drosophila melanogaster | PMID:16326860 | BDGP iPCR Collection | DGRC | 2026-08-29 04:56:16 | 0 | ||||
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IP04838 Resource Report Resource Website |
RRID:DGRC_1381486 | Drosophila melanogaster | PMID:16326860 | BDGP iPCR Collection | DGRC | 2026-08-29 04:59:56 | 0 | ||||
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Anti-VGlut3 [N34/34.2R] Resource Report Resource Website |
RRID:Addgene_114494 | anti-VGlut3 (Rattus norvegicus) recombinant mouse monoclonal antibody | Mus musculus | Ampicillin | PMID:30667360 | RRID: AB_2750780. Isotype: IgG2a. Additional species: mouse, rat. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. | Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:15 | 0 | |
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Anti-NGL-3/LRRC4B [N51/6.2R] Resource Report Resource Website |
RRID:Addgene_114493 | anti-NGL-3/LRRC4B (Rattus norvegicus) recombinant mouse monoclonal antibody | Mus musculus | Ampicillin | PMID:30667360 | RRID: AB_2750799. Isotype: IgG2a. Additional species: rat, mouse. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. | Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:15 | 0 | |
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AICSDP-49: MYL2-mEGFP Resource Report Resource Website |
RRID:Addgene_114414 | MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette | Homo sapiens | Ampicillin | PMID:30956114 | This plasmid has been used with locus-specific CRISPR/Cas9 in a two-step editing process to add a mEGFP tag to the C-terminus of human MYL2 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After initial HDR, stable expression of CAGGS-driven mCherry will indicate cells with integrated donor sequence. This selection cassette is designed to be removed with subsequent CRISPR/Cas9-mediated excision as previously described (https://doi.org/10.1101/342881). A linker of 3-21 amino acids will be produced after excision. To enrich for edited cells, see our protocol for fluorsecence-assisted cell sorting and subcloning of hiPSCs (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/. Please visit https://www.biorxiv.org/content/early/2018/06/09/342881 for the bioRxiv preprint. | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used | Addgene | 2026-10-17 02:33:14 | 0 |
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AICSDP-34: TNNI1-mEGFP Resource Report Resource Website |
RRID:Addgene_114411 | TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette | Homo sapiens | Ampicillin | PMID:30956114 | This plasmid has been used with locus-specific CRISPR/Cas9 in a two-step editing process to add a mEGFP tag to the C-terminus of human TNNI1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After initial HDR, stable expression of hPGK-driven mCherry will indicate cells with integrated donor sequence. This selection cassette is designed to be removed with subsequent CRISPR/Cas9-mediated excision as previously described (https://doi.org/10.1101/342881). A linker of 3-21 amino acids will be produced after excision. To enrich for edited cells, see our protocol for fluorsecence-assisted cell sorting and subcloning of hiPSCs (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/. Please visit https://www.biorxiv.org/content/early/2018/06/09/342881 for the bioRxiv preprint. | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:14 | 0 | |
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iLID-eGFP-2xC2-iLID Resource Report Resource Website |
RRID:Addgene_114418 | iLID domain | Synthetic | Kanamycin | PMID:30182785 | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-17 02:33:14 | 0 | ||
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iLID-eGFP-3xC2 Resource Report Resource Website |
RRID:Addgene_114417 | iLID domain | Synthetic | Kanamycin | PMID:30182785 | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-17 02:33:14 | 0 | ||
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iLID-eGFP-C2 Resource Report Resource Website |
RRID:Addgene_114415 | iLID domain | Synthetic | Kanamycin | PMID:30182785 | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-17 02:33:14 | 0 | ||
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pAAVS1-TRE-MA4-GFP Resource Report Resource Website |
RRID:Addgene_114380 | MLL-AF4 fusion gene | Homo sapiens | Ampicillin | PMID:29386396 | Backbone Marker:Rudolf Jaenisch, Addgene Plasmid #22075; Backbone Size:6602; Vector Backbone:AAVS1 SA-2A-puro-pA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:13 | 0 | ||
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Anti-Synaptotagmin-12 [N277/7R] Resource Report Resource Website |
RRID:Addgene_114482 | anti-Synaptotagmin-12 (Mus musculus) recombinant mouse monoclonal antibody | Mus musculus | Ampicillin | PMID:30667360 | RRID: AB_2750763. Isotype: IgG2a. Additional species: mouse, rat. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. | Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:15 | 0 | |
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Anti-SUR2B [N323B/20R] Resource Report Resource Website |
RRID:Addgene_114480 | anti-SUR2B (Rattus norvegicus) recombinant mouse monoclonal antibody | Mus musculus | Ampicillin | PMID:30667360 | RRID: AB_2750775. Isotype: IgG2a. Additional species: rat, mouse, human. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. | Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:15 | 0 | |
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Anti-Botch [N116/14R] Resource Report Resource Website |
RRID:Addgene_114487 | anti-Botch (Rattus norvegicus) recombinant mouse monoclonal antibody | Mus musculus | Ampicillin | PMID:30667360 | RRID: AB_2750705. Isotype: IgG2a. Additional species: rat, mouse, human. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. | Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:15 | 0 | |
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Anti-Flrt3 [N353/13R] Resource Report Resource Website |
RRID:Addgene_114486 | anti-Flrt3 (Mus musculus) recombinant mouse monoclonal antibody | Mus musculus | Ampicillin | PMID:30667360 | RRID: AB_2750784. Isotype: IgG2a. Additional species: mouse. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. | Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:15 | 0 | |
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AICSDP-29: LMNB1-mTagRFP-T Resource Report Resource Website 1+ mentions |
RRID:Addgene_114403 | LMNB1 Homology Arms with mTagRFPT-linker | Homo sapiens | Ampicillin | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human LMNB1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRSRAQAS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used | Addgene | 2026-10-17 02:33:14 | 1 |
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