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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Authority Record Last Update Mentions Count
pAW-CD8-NbVHH05-GFP
 
Resource Report
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RRID:DGRC_1562 Drosophila Ampicillin Nanobody, pAW backbone, Nanobody expression in fly cells, GFP, CD8 Drosophila Genomics Resource Center 2026-09-05 03:58:48 0
pUAST-aC-EGFP
 
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RRID:DGRC_1009 Drosophila Ampicillin PMID:11102367 Epitope Tagged Vectors, UAS/GAL4 Vectors, Recombination and Gateway Vectors, General Fly Transformation Vectors, white[+], P-elements, UAS enhancer, hsp70 promoter, SV40 3'UTR, eGFP tag Drosophila Genomics Resource Center 2026-09-05 03:58:44 0
pUAST-aC-V5/6xHis
 
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RRID:DGRC_1012 Drosophila Ampicillin Epitope Tagged Vectors, UAS/GAL4 Vectors, Recombination and Gateway Vectors, General Fly Transformation Vectors, white[+], P-elements, UAS enhancer, hsp70 promoter, SV40 3'UTR, V5 tag, 6X His tag Drosophila Genomics Resource Center 2026-09-05 03:58:44 0
pUAST-aC-ECFP
 
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RRID:DGRC_1011 Drosophila Ampicillin PMID:11532957 Epitope Tagged Vectors, UAS/GAL4 Vectors, Recombination and Gateway Vectors, General Fly Transformation Vectors, white[+], P-elements, UAS enhancer, hsp70 promoter, SV40 3'UTR, eCFP tag Drosophila Genomics Resource Center 2026-09-05 03:58:44 0
pUAST-aC-EYFP
 
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RRID:DGRC_1010 Drosophila Ampicillin PMID:10585440 Epitope Tagged Vectors, UAS/GAL4 Vectors, Recombination and Gateway Vectors, General Fly Transformation Vectors, white[+], P-elements, UAS enhancer, hsp70 promoter, SV40 3'UTR, eYFP tag Drosophila Genomics Resource Center 2026-09-05 03:58:44 0
IP16479
 
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RRID:DGRC_1604016 Drosophila melanogaster PMID:16326860 BDGP iPCR Collection DGRC 2026-08-29 04:56:16 0
IP04838
 
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RRID:DGRC_1381486 Drosophila melanogaster PMID:16326860 BDGP iPCR Collection DGRC 2026-08-29 04:59:56 0
Anti-VGlut3 [N34/34.2R]
 
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RRID:Addgene_114494 anti-VGlut3 (Rattus norvegicus) recombinant mouse monoclonal antibody Mus musculus Ampicillin PMID:30667360 RRID: AB_2750780. Isotype: IgG2a. Additional species: mouse, rat. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:33:15 0
Anti-NGL-3/LRRC4B [N51/6.2R]
 
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RRID:Addgene_114493 anti-NGL-3/LRRC4B (Rattus norvegicus) recombinant mouse monoclonal antibody Mus musculus Ampicillin PMID:30667360 RRID: AB_2750799. Isotype: IgG2a. Additional species: rat, mouse. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:33:15 0
AICSDP-49: MYL2-mEGFP
 
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RRID:Addgene_114414 MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette Homo sapiens Ampicillin PMID:30956114 This plasmid has been used with locus-specific CRISPR/Cas9 in a two-step editing process to add a mEGFP tag to the C-terminus of human MYL2 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After initial HDR, stable expression of CAGGS-driven mCherry will indicate cells with integrated donor sequence. This selection cassette is designed to be removed with subsequent CRISPR/Cas9-mediated excision as previously described (https://doi.org/10.1101/342881). A linker of 3-21 amino acids will be produced after excision. To enrich for edited cells, see our protocol for fluorsecence-assisted cell sorting and subcloning of hiPSCs (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/. Please visit https://www.biorxiv.org/content/early/2018/06/09/342881 for the bioRxiv preprint. Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin homology arms contain point mutations to disrupt crRNA binding sites used Addgene 2026-10-17 02:33:14 0
AICSDP-34: TNNI1-mEGFP
 
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RRID:Addgene_114411 TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette Homo sapiens Ampicillin PMID:30956114 This plasmid has been used with locus-specific CRISPR/Cas9 in a two-step editing process to add a mEGFP tag to the C-terminus of human TNNI1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After initial HDR, stable expression of hPGK-driven mCherry will indicate cells with integrated donor sequence. This selection cassette is designed to be removed with subsequent CRISPR/Cas9-mediated excision as previously described (https://doi.org/10.1101/342881). A linker of 3-21 amino acids will be produced after excision. To enrich for edited cells, see our protocol for fluorsecence-assisted cell sorting and subcloning of hiPSCs (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/. Please visit https://www.biorxiv.org/content/early/2018/06/09/342881 for the bioRxiv preprint. Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:33:14 0
iLID-eGFP-2xC2-iLID
 
Resource Report
Resource Website
RRID:Addgene_114418 iLID domain Synthetic Kanamycin PMID:30182785 Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Addgene 2026-10-17 02:33:14 0
iLID-eGFP-3xC2
 
Resource Report
Resource Website
RRID:Addgene_114417 iLID domain Synthetic Kanamycin PMID:30182785 Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Addgene 2026-10-17 02:33:14 0
iLID-eGFP-C2
 
Resource Report
Resource Website
RRID:Addgene_114415 iLID domain Synthetic Kanamycin PMID:30182785 Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Addgene 2026-10-17 02:33:14 0
pAAVS1-TRE-MA4-GFP
 
Resource Report
Resource Website
RRID:Addgene_114380 MLL-AF4 fusion gene Homo sapiens Ampicillin PMID:29386396 Backbone Marker:Rudolf Jaenisch, Addgene Plasmid #22075; Backbone Size:6602; Vector Backbone:AAVS1 SA-2A-puro-pA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:33:13 0
Anti-Synaptotagmin-12 [N277/7R]
 
Resource Report
Resource Website
RRID:Addgene_114482 anti-Synaptotagmin-12 (Mus musculus) recombinant mouse monoclonal antibody Mus musculus Ampicillin PMID:30667360 RRID: AB_2750763. Isotype: IgG2a. Additional species: mouse, rat. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:33:15 0
Anti-SUR2B [N323B/20R]
 
Resource Report
Resource Website
RRID:Addgene_114480 anti-SUR2B (Rattus norvegicus) recombinant mouse monoclonal antibody Mus musculus Ampicillin PMID:30667360 RRID: AB_2750775. Isotype: IgG2a. Additional species: rat, mouse, human. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:33:15 0
Anti-Botch [N116/14R]
 
Resource Report
Resource Website
RRID:Addgene_114487 anti-Botch (Rattus norvegicus) recombinant mouse monoclonal antibody Mus musculus Ampicillin PMID:30667360 RRID: AB_2750705. Isotype: IgG2a. Additional species: rat, mouse, human. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:33:15 0
Anti-Flrt3 [N353/13R]
 
Resource Report
Resource Website
RRID:Addgene_114486 anti-Flrt3 (Mus musculus) recombinant mouse monoclonal antibody Mus musculus Ampicillin PMID:30667360 RRID: AB_2750784. Isotype: IgG2a. Additional species: mouse. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:33:15 0
AICSDP-29: LMNB1-mTagRFP-T
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_114403 LMNB1 Homology Arms with mTagRFPT-linker Homo sapiens Ampicillin This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human LMNB1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRSRAQAS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin homology arms contain point mutations to disrupt crRNA binding sites used Addgene 2026-10-17 02:33:14 1

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