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Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike protein
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:2900; Vector Backbone:pRSET5A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35123472
Proper citation: RRID:Addgene_171709 Copy
Species: SARS-CoV-2
Genetic Insert: Spike of B.1.617.1 strain
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34519517
Proper citation: RRID:Addgene_172319 Copy
Species: SARS-CoV-2
Genetic Insert: Spike of B.1.617.2 strain
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34519517
Proper citation: RRID:Addgene_172320 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Nucleocapsid phosphoprotein
Vector Backbone Description: Backbone Size:6502; Vector Backbone:MscV-IRES-mcherry; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34257311
Comments: Fareh lab cloned this construct for this study (BioRxiv--Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance)
Proper citation: RRID:Addgene_173030 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Receptor Binding domain
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5442; Vector Backbone:pET21b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33532775
Proper citation: RRID:Addgene_167126 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp3 (residues 1584-1945)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167261 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp3 (residues 1089-1203)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167260 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp15 (residues 1-346)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167265 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp9 (residues 1-113)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167264 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp3 (residues 413-676)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167258 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp1 (residues 13-127)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33319167
Proper citation: RRID:Addgene_167256 Copy
Species: SARS-CoV-2
Genetic Insert: N protein (residues 1-419)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167255 Copy
Species: SARS-CoV-2
Genetic Insert: nsp10, nsp16
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33423577
Comments: SGC clone sample ID: nsp16_SARS2-nsp10_SARS2-MVC019-A07:C245477
Proper citation: RRID:Addgene_167849 Copy
Species: SARS-CoV-2
Genetic Insert: nsp10
Vector Backbone Description: Vector Backbone:pCOLADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198326
Comments: Unlike SARS-CoV-2 nsp10 that gets expressed in the context of a larger polypeptide, this SARS-CoV-2 nsp10 gets expressed from a 5' ATG, and thus might contain a methionine at the N-terminus that is not present in the nsp5-cleaved nsp10 in cells.
Proper citation: RRID:Addgene_169158 Copy
Species: SARS-CoV-2
Genetic Insert: 3xFlag-nsp5CS[VATLQ]-nsp14
Vector Backbone Description: Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198326
Comments: We have included the predicted cleavage site for nsp5 found between nsp13 and nsp14 (VATLQ) between the 3xFlag tag and nsp14 in this construct, which should allow a purified nsp5 protease to cleave-out the 3xFlag tag similarly to what might happen in cells.
Proper citation: RRID:Addgene_169159 Copy
Species: SARS-CoV-2
Genetic Insert: 3xFlag-His-nsp5CS-nsp9
Vector Backbone Description: Vector Backbone:pLIB (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198327
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169177 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Nsp3 Mac1
Vector Backbone Description: Backbone Size:5358; Vector Backbone:pET22b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33853786
Proper citation: RRID:Addgene_169209 Copy
Species: SARS-CoV-2
Genetic Insert: 14His-SUMO-nsp7
Vector Backbone Description: Vector Backbone:pK27Sumo; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198323
Proper citation: RRID:Addgene_169186 Copy
Species: SARS-CoV-2
Genetic Insert: CoV-2 nsp1
Vector Backbone Description: Backbone Size:5163; Vector Backbone:pCDNA4TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34624207
Proper citation: RRID:Addgene_176057 Copy
Species: SARS-CoV-2
Genetic Insert: CoV-2 nsp1 R124A/K125A
Vector Backbone Description: Backbone Size:5163; Vector Backbone:pCDNA4TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34624207
Proper citation: RRID:Addgene_176059 Copy
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