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  • Organism:sars-cov-2 (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
CBM9-ID-H1
 
Resource Report
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RRID:Addgene_171709 SARS-CoV-2 Spike protein SARS-CoV-2 Chloramphenicol and Ampicillin PMID:35123472 Backbone Marker:Thermo Fisher; Backbone Size:2900; Vector Backbone:pRSET5A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:10:20 0
pcDNA3.3-SARS2-B.1.617.1
 
Resource Report
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1+ mentions
RRID:Addgene_172319 Spike of B.1.617.1 strain SARS-CoV-2 Ampicillin PMID:34519517 Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H, H1101D, c-terminal 18aa deletion 2026-08-15 01:10:23 3
pcDNA3.3-SARS2-B.1.617.2
 
Resource Report
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10+ mentions
RRID:Addgene_172320 Spike of B.1.617.2 strain SARS-CoV-2 Ampicillin PMID:34519517 Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin T19R, 156G, 157-158del, L452R, T478K, D614G, P681R, D950N, c-terminal 18aa deletion 2026-08-15 01:10:23 37
SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry
 
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RRID:Addgene_173030 SARS-CoV-2 Nucleocapsid phosphoprotein SARS-CoV-2 Ampicillin PMID:34257311 Fareh lab cloned this construct for this study (BioRxiv--Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance) Backbone Size:6502; Vector Backbone:MscV-IRES-mcherry; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:10:30 0
pET21b-RBD-JA1
 
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Resource Website
RRID:Addgene_167126 SARS-CoV-2 Receptor Binding domain SARS-CoV-2 Ampicillin PMID:33532775 Backbone Marker:Novagen; Backbone Size:5442; Vector Backbone:pET21b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin residues (333 to 529 aa) 2026-08-15 01:09:41 0
pMCSG53_Nsp3_Y_1
 
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RRID:Addgene_167261 Nsp3 (residues 1584-1945) SARS-CoV-2 Ampicillin Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin none 2026-08-15 01:09:42 0
pMCSG53_Nsp3_NAB_2
 
Resource Report
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RRID:Addgene_167260 Nsp3 (residues 1089-1203) SARS-CoV-2 Ampicillin Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin none 2026-08-15 01:09:42 0
pMCSG53_Nsp15
 
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RRID:Addgene_167265 Nsp15 (residues 1-346) SARS-CoV-2 Ampicillin Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin none 2026-08-15 01:09:42 0
pMCSG53_Nsp9
 
Resource Report
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RRID:Addgene_167264 Nsp9 (residues 1-113) SARS-CoV-2 Ampicillin Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin none 2026-08-15 01:09:42 0
pMCSG53_Nsp3_Unique
 
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RRID:Addgene_167258 Nsp3 (residues 413-676) SARS-CoV-2 Ampicillin Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin none 2026-08-15 01:09:42 0
pMCSG53_Nsp1
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_167256 Nsp1 (residues 13-127) SARS-CoV-2 Ampicillin PMID:33319167 Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin none 2026-08-15 01:09:42 2
pMCSG53_N
 
Resource Report
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RRID:Addgene_167255 N protein (residues 1-419) SARS-CoV-2 Ampicillin Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin none 2026-08-15 01:09:42 0
pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2
 
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RRID:Addgene_167849 nsp10, nsp16 SARS-CoV-2 Kanamycin PMID:33423577 SGC clone sample ID: nsp16_SARS2-nsp10_SARS2-MVC019-A07:C245477 Backbone Marker:SGC (Addgene Plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:47 0
pCOLADuet-1_nsp10 (SARS-CoV-2)
 
Resource Report
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RRID:Addgene_169158 nsp10 SARS-CoV-2 Kanamycin PMID:34198326 Unlike SARS-CoV-2 nsp10 that gets expressed in the context of a larger polypeptide, this SARS-CoV-2 nsp10 gets expressed from a 5' ATG, and thus might contain a methionine at the N-terminus that is not present in the nsp5-cleaved nsp10 in cells. Vector Backbone:pCOLADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Codon optimised for E. coli 2026-08-15 01:09:57 0
pET11a_3xFlag-nsp14 (SARS-CoV-2)
 
Resource Report
Resource Website
RRID:Addgene_169159 3xFlag-nsp5CS[VATLQ]-nsp14 SARS-CoV-2 Ampicillin PMID:34198326 We have included the predicted cleavage site for nsp5 found between nsp13 and nsp14 (VATLQ) between the 3xFlag tag and nsp14 in this construct, which should allow a purified nsp5 protease to cleave-out the 3xFlag tag similarly to what might happen in cells. Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Codon optimised for E. coli 2026-08-15 01:09:57 0
pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)
 
Resource Report
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RRID:Addgene_169177 3xFlag-His-nsp5CS-nsp9 SARS-CoV-2 Ampicillin PMID:34198327 Baculovirus generation using Tn7 transposition (Bac-to-Bac). Vector Backbone:pLIB (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Codon optimised for insect cells (Spodoptera frugiperda) 2026-08-15 01:09:57 0
Nsp3 Mac1 - C2 (UCSF)
 
Resource Report
Resource Website
RRID:Addgene_169209 SARS-CoV-2 Nsp3 Mac1 SARS-CoV-2 Ampicillin PMID:33853786 Backbone Size:5358; Vector Backbone:pET22b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:58 0
pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)
 
Resource Report
Resource Website
RRID:Addgene_169186 14His-SUMO-nsp7 SARS-CoV-2 Kanamycin PMID:34198323 Vector Backbone:pK27Sumo; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Codon optimised for E. coli 2026-08-15 01:09:57 0
pCDNA4 CoV-2 nsp1 3xFLAG
 
Resource Report
Resource Website
RRID:Addgene_176057 CoV-2 nsp1 SARS-CoV-2 Ampicillin PMID:34624207 Backbone Size:5163; Vector Backbone:pCDNA4TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:54 0
pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG
 
Resource Report
Resource Website
RRID:Addgene_176059 CoV-2 nsp1 R124A/K125A SARS-CoV-2 Ampicillin PMID:34624207 Backbone Size:5163; Vector Backbone:pCDNA4TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin R124A and K125A 2026-08-15 01:10:54 0

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