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Species: E. coli
Genetic Insert: tdNfsB(F124W)-mCherry
Vector Backbone Description: Backbone Marker:Scott Sternson; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30111097
Comments: Sternson Lab recommendations for propagation of AAV-FLEX plasmids: "These vectors are prone to recombination. This is a well known issue with these AAV vectors and is due to the inverted terminal repeats (ITRs) required for AAV production. To minimize recombination, we propagate these plasmids in NEB Stable cells. Also, to minimize recombination, cells should be cultured at 30 C.
Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended.
Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed."
Proper citation: RRID:Addgene_113762 Copy
Species: E. coli
Genetic Insert: clbO
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114153 Copy
Species: E. coli
Genetic Insert: clbK
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114152 Copy
Species: E. coli
Genetic Insert: clbH
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28805802
Proper citation: RRID:Addgene_114157 Copy
Species: E. coli
Genetic Insert: clbB
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28805802
Proper citation: RRID:Addgene_114155 Copy
Species: E. coli
Genetic Insert: ClbD
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114146 Copy
Species: E. coli
Genetic Insert: ClbF
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114148 Copy
Species: E. coli
Genetic Insert: ClbE
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114147 Copy
Species: E. coli
Genetic Insert: OsmC
Vector Backbone Description: Backbone Size:5386; Vector Backbone:pSKB3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15103136
Comments: Please note that the plasmid pSKB3.EG10680 contains a Kanamycin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
Proper citation: RRID:Addgene_11436 Copy
Species: E. coli
Genetic Insert: BirA
Vector Backbone Description: Backbone Marker:InVitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22412018
Proper citation: RRID:Addgene_35700 Copy
Species: E. coli
Genetic Insert: BirA(R118G)-HA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5333; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22412018
Proper citation: RRID:Addgene_36047 Copy
Species: e. coli
Genetic Insert: lac repressor
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4486; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20576148
Proper citation: RRID:Addgene_45393 Copy
Species: E. coli
Genetic Insert: LexAp65
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2551; Vector Backbone:pDONR P5-P2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_41437 Copy
Species: E. coli
Genetic Insert: LplA(AILR) with ER-retention sequence KDEL
Vector Backbone Description: Backbone Size:4261; Vector Backbone:pCAG; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23181687
Proper citation: RRID:Addgene_42574 Copy
Species: E. coli
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:Promega Corporation; Backbone Size:2700; Vector Backbone:pCI; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16397505
Proper citation: RRID:Addgene_44172 Copy
Species: E. coli
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:Promega Corporation; Backbone Size:2700; Vector Backbone:pCI; Vector Types:Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23319179
Proper citation: RRID:Addgene_44167 Copy
Species: E. coli
Genetic Insert: EcoRI endonuclease & EcoRI methylase
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:3000; Vector Backbone:pKC30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:6088551
Comments: pKC30 plasmid derivation is described in Nature (1981) 292, 128-132.
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_39993 Copy
Species: E. coli
Genetic Insert: gusA
Vector Backbone Description: Vector Backbone:RP4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:22820336
Comments: GenBank accession number JQ895026
Multiple-cloning site (MCS) of pLMB51: GATTACCTCA GTCTAGAGCT CGAGAAGCTT GGATCCATGG TACCCTACA
Proper citation: RRID:Addgene_40083 Copy
Species: E. coli
Genetic Insert: GFP-LacI**
Vector Backbone Description: Backbone Marker:Andrew W. Murray Lab, UCSF (Straight, Sedat, & Murray, 1998. PMID: 9813090); Backbone Size:4600; Vector Backbone:pAFS135; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21724830
Comments: The plasmid pAFS135 (Straight, Sedat, and Murray, 1998) contains GFP(S65T, V163A)-LacI(P3Y, del C-terminal 11 aa)-SV40 NLS under the control of a HIS3 promoter fragment. To construct pAT123, the HIS3 marker of AFS135 was replaced with a ScaI-DraIII fragment from RS405 containing LEU2 (a kind gift from Karine Dubrana and Susan Gasser).
The plasmid pAT222 (Addgene plasmid #40942) was obtained by site-directed mutagenesis of pAT123 to create GFP-LacI**, using the primers (base mutations in lowercase):
5’- GTGGCACAgCAACTGGCGGaCAAAggtggcggaTCGTTGCTGATTGGCGTTGCCtCCT-3’ and
5’- AGGaGGCAACGCCAATCAGCAACGAtccgccaccTTTGtCCGCCAGTTGcTGTGCCAC- 3’.
The LacI** variant was then subcloned into pAFS135 using NotI and XhoI restriction sites to create plasmid pAT253.
Proper citation: RRID:Addgene_40943 Copy
Species: E. coli
Genetic Insert: CsrA
Vector Backbone Description: Backbone Marker:Lim Lab; Vector Backbone:pZ with p15a origin; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23878244
Proper citation: RRID:Addgene_37571 Copy
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