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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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E. coli BL21 (DE3) ΔEntD Resource Report Resource Website |
RRID:Addgene_192874 | The strain has a 535 nt deletion between nt 15 and nt 551 in the EntD gene. | E.coli | None | PMID:16709676 | EntD, a 4'-phosphopantetheinyl transferase, is required for native enterobactin synthesis. This strain allows for expression of nonribosomal peptide synthetase (NRPS) carrier proteins that do not harbor a phosphopantetheinyl group. | Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:None | Addgene | 2026-10-17 03:00:16 | 0 | |
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S4197 Resource Report Resource Website |
RRID:Addgene_200839 | Genotype: MG1655 rph+, ilvG+, ΔlacZ | Escherichia coli str. K-12 substr. MG1655 | None | PMID:20952573 | Corresponding wild-type strain (rapZ+, glmZ+, glmY+) for strain Z956 (Addgene Bacterial strain #200838). | Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:None | Addgene | 2026-10-17 02:59:45 | 0 | |
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E. coli MEV15 Resource Report Resource Website |
RRID:Addgene_197112 | None | E. coli MEV15 is engineered to host sesquiterpenoid biosynthetic pathways. Sesquiterpenoid production is achieved by supplying the strain with plasmids encoding terpene cyclase and cytochrome P450s under the control of Marionette promoters (See https://www.addgene.org/kits/marionette-sensor-collection/ and 10.1038/s41589-018-0168-3). Sesquiterpenoid production can be induced by IPTG, vanillic aci, and other inducers controlling cytorhcome P450s. The strain has the upper MEV pathway from pMevT (addgene #17815) inserted into 4418413/4418414, the lower MEV pathway from pMBIS (addgene #17817) and E. coli ispA inserted into 4105665/4105664, the designed redox enzyme array inserteed into 3801913/3801912, and the Marionette cluster from sAJM.1506 (addgene #108254) inserted into 3753777/3752159 of E. coli BL21(DE3)'s genome. The nucleotide numbers are based on NCBI accession # NZ_CP053602. The redox enzyme array consists of fprD/fdxD from Streptomyces avermitilis, fpr/fldA from E. coli, fenr/fer1 from spinach chloroplasts, abd pdr/pdx (camA/camB) from Pseudomonas putida. The Marionette cluster was transferred using phage transduction, resulting in the replacement of nucleotides between 3745758/3839292 by those in the corresponding regions from sAJM.1506 (parent strain: E. coli MG1655), as evident by whole-genome sequencing. | Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None | Addgene | 2026-10-17 02:59:22 | 0 | ||||
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B-95.ΔAΔfabR Resource Report Resource Website 1+ mentions |
RRID:Addgene_197934 | RNA polymerase gene (T7 phage)/chromosome | None | PMID:25982672 | Genotype: The same as BL21(DE3) except for the additional mutations at 95 UAG codons, disruption of prfA, and frameshift in fabR. | Vector Backbone:BL21(DE3); Vector Types:; Bacterial Resistance:None | Addgene | 2026-10-17 02:57:41 | 1 | ||
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BL21∆serB Resource Report Resource Website 1+ mentions |
RRID:Addgene_34929 | None | PMID:21868676 | To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which catalyzes the last step in serine biosynthesis, was deleted from Escherichia coli strain BL21. Markerless gene deletions were carried out using a λ-red and FLP recombinase-based gene knockout strategy. | Vector Backbone:None; Vector Types:; Bacterial Resistance:None | Addgene | 2026-10-17 02:45:12 | 5 | |||
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BLIM cells Resource Report Resource Website 1+ mentions |
RRID:Addgene_35609 | none | None | PMID:10610690 | To be used with the following plasmids from the Matthews lab: pTARA (www.addgene.org/31491), pLS1 (www.addgene.org/31490), and (www.addgene.org/31492) pLS1/-11 | Vector Backbone:NA; Vector Types:; Bacterial Resistance:None | Addgene | 2026-10-17 02:45:20 | 1 | ||
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IF189 Resource Report Resource Website |
RRID:Addgene_134836 | lysogen of a temperature-inducible bacteriophage lambda | None | PMID:28522818 | Genotype is [λ+ Lac- galK2 IN(rrnD-rrnE)1 rph-1], a derivative of strain W3102 Phenotypic assay: Grow at 30°C, but restricted at 42°C due to the induction of phage particles and cell lysis. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-10-17 02:36:51 | 0 | ||
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MG1655-OptoCre-bla-P-R Resource Report Resource Website |
RRID:Addgene_188474 | P-R-lox-TT-lox-bla | None | PMID:36823420 | Please visit https://www.biorxiv.org/content/10.1101/2022.06.10.495621v1 for bioRxiv preprint. | Vector Backbone:E. coli K-12 MG1655; Vector Types:; Bacterial Resistance:None | Addgene | 2026-10-17 02:57:23 | 0 | ||
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MG1655-OptoCre-knt-P-R Resource Report Resource Website |
RRID:Addgene_188475 | P-R-lox-TT-lox-knt | None | PMID:36823420 | Please visit https://www.biorxiv.org/content/10.1101/2022.06.10.495621v1 for bioRxiv preprint. | Vector Backbone:E. coli K-12 MG1655; Vector Types:; Bacterial Resistance:None | Addgene | 2026-10-17 02:57:23 | 0 | ||
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MG1655-OptoCre-knt-P*-R Resource Report Resource Website |
RRID:Addgene_188476 | P*-R-lox-TT-lox-knt | None | PMID:36823420 | Please visit https://www.biorxiv.org/content/10.1101/2022.06.10.495621v1 for bioRxiv preprint. | Vector Backbone:E. coli K-12 MG1655; Vector Types:; Bacterial Resistance:None | Addgene | 2026-10-17 02:57:23 | 0 | ||
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CLM24 ∆lpxM Resource Report Resource Website |
RRID:Addgene_132780 | None | PMID:33536221 | Genotype: W3110 ∆waaL ∆lpxM | Vector Backbone:Strain; Vector Types:; Bacterial Resistance:None | Addgene | 2026-10-17 02:56:36 | 0 | |||
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bSLS.114 Resource Report Resource Website 1+ mentions |
RRID:Addgene_191530 | E. coli B F– ompT gal dcm lon hsdSB(rB–mB–) [malB+]K-12(λS) araB::T7RNAP-tetA Δretron-Eco1 | E.coli | None | PMID:34949838 | Derived from BL21(AI), grow in LB in BSL1 laboratory conditions Genotype: E. coli B F– ompT gal dcm lon hsdSB(rB–mB–) [malB+]K-12(λS) araB::T7RNAP-tetA Δretron-Eco1 Genotyping primers: CATGTGCATGAAAACCACTGC / CTGGTTGGACGAAGAAGTGC (273 base amplicon) | Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:None | Addgene | 2026-10-17 02:56:33 | 2 | |
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BW-Para Resource Report Resource Website |
RRID:Addgene_172602 | None | PMID:34369028 | The BW-Para E. coli strain is used to screen the functions of chimeric AraC/XylS transcription activators using beta-galactosidase assays (after growing transformed cells on MOPS media). Plasmids encoding these chimeras are found at https://www.addgene.org/browse/article/28216962/. The protocol for the reporter assay is detailed in the manuscript. The genotype for BW-Para is [F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514], ΔlacI785::kan, ΔaraC771::kan, ΔrhaSR::(PBADmut:lacZ)]. The PBADmut:lacZ reporter construct integrated into the rhaSR KO locus comprises a synthetic Para-I promoter with -10/-35 sites of GATACT/TTTACA respectively and an ara-I DNA binding site proximal to the -35 site. The integrated 3.5 kb cassette coding for the reporter construct can be amplified from the genome using the primers: (forward) 5' - GGTGAAAGTTGGAACCTCTTAC - 3' and (reverse) 5'- GCGAGGAAGCGGAATATATCCCC - 3'. Cells should be freshly transformed prior to each assay. | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | Addgene | 2026-10-17 02:56:55 | 0 | |||
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pMBA332 Resource Report Resource Website |
RRID:Addgene_214744 | BBa_J23119-RiboJ-RBS(21992)-gfpmut3 | other | None | PMID:38086386 | Please note: Plasmid contains three mutations in Rep101. These mutations are not known to affect plasmid function. | Vector Backbone:pSC101 ; Vector Types:Bacterial Expression; Bacterial Resistance:None | Addgene | 2026-10-17 03:02:18 | 0 | |
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pMBA333 Resource Report Resource Website |
RRID:Addgene_214745 | BBa_J23119-RiboJ-RBS(21992)-gfpmut3 | other | None | PMID:38086386 | Vector Backbone:p15A ; Vector Types:Bacterial Expression; Bacterial Resistance:None | Addgene | 2026-10-17 03:02:18 | 0 | ||
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pMBA329 Resource Report Resource Website |
RRID:Addgene_214749 | BBa_J23119-RBS(22821)-mcherry | other | None | PMID:38086386 | Please note: Plasmid contains mutations in the five C-terminal amino acids of mCherry. These mutations are not known to affect plasmid function. | Vector Backbone:colE1 ; Vector Types:Bacterial Expression; Bacterial Resistance:None | Addgene | 2026-10-17 03:02:18 | 0 | |
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ADP1-ISx Resource Report Resource Website |
RRID:Addgene_216551 | None | None | PMID:28667117 | The edited genome file is available on this GitHub repository: https://github.com/barricklab/Abaylyi-EE. | Vector Backbone:None; Vector Types:; Bacterial Resistance:None | Addgene | 2026-10-17 03:02:22 | 0 | ||
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eScaf Resource Report Resource Website |
RRID:Addgene_220921 | Genotype: F’[traD36 lacIq lacZ ∆M15 proA+B+] glnV (supE) thi-1 ∆(mcrB-hsdSM)5 (rK- mK- McrB-) ∆(lac-proAB) ulaD::M13 | E. coli | None | PMID:38499480 | Primers for validation Pair 1: agtaaggacgcgccatgaaa + agcgaaagacagcatcggaa (Ta = 59 C, should have 2526 bp product) Pair 2: aatcggttgaatgtcgccct + gggaaacgacgatgagcaga (Ta = 59, should have 3900 bp product) | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | Addgene | 2026-10-17 03:02:52 | 0 | |
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B-95.ΔA Resource Report Resource Website |
RRID:Addgene_197933 | RNA polymerase gene (T7 phage)/chromosome | None | PMID:25982672 | Genotype: The same as BL21(DE3) except for the additional mutations at 95 UAG codons and disruption of the prfA gene | Vector Backbone:BL21(DE3); Vector Types:; Bacterial Resistance:None | Addgene | 2026-10-17 03:00:54 | 0 | ||
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MG1655 ΔendA ΔrecA Resource Report Resource Website |
RRID:Addgene_37853 | None | PMID:20643967 | Vector Backbone:N/A; Vector Types:E. coli bacterial strain; Bacterial Resistance:None | Addgene | 2026-10-17 02:45:39 | 0 |
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