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Species: Synthetic
Genetic Insert: CspRecT / Bxb-1
Vector Backbone Description: Vector Backbone:pKM461; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
Comments: Replicating ORBIT V1 helper plasmid w/ inducible oligo recombineering module (m-tol) and bxb-1 integrase (arabinose). SacB is present for counterselection and gentamicin is the resistance marker. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint.
Proper citation: RRID:Addgene_205291 Copy
Genetic Insert: TevSpCas9, sgRNA cassette, oriT, CenArsHis
Vector Backbone Description: Backbone Size:52707; Vector Backbone:pTA-Mob; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:31586051
Proper citation: RRID:Addgene_202796 Copy
Vector Backbone Description: Backbone Size:57000; Vector Backbone:pTA-Mob 2.0; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37850145
Proper citation: RRID:Addgene_188617 Copy
Species: Hemophilus influenzae
Genetic Insert: HindII
Vector Backbone Description: Backbone Size:57000; Vector Backbone:pTA-Mob 2.0; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology, Diatom Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37850145
Proper citation: RRID:Addgene_188625 Copy
Species: Homo sapiens
Genetic Insert: SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
Vector Backbone Description: Vector Backbone:pMMACE DEST CMV SpCas9; Vector Types:Mammalian Expression, CRISPR, Recombinant baculovirus production; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35801912
Proper citation: RRID:Addgene_206267 Copy
Species: Other
Genetic Insert: H2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)
Vector Backbone Description: Vector Backbone:pMMACE DEST CMV H2B IRFP713; Vector Types:Mammalian Expression, Recombinant baculovirus production, CRE Acceptor (MultiBac); Bacterial Resistance:Gentamicin
Defining Citation: PMID:35801912
Proper citation: RRID:Addgene_206266 Copy
Species: Homo sapiens
Genetic Insert: H2B iRFP713, mAG-hGeminin, mKO2-hCdt1
Vector Backbone Description: Vector Backbone:pMMACE DEST CMV H2B IRFP713; Vector Types:Mammalian Expression, Recombinant baculovirus production; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35801912
Comments: L224Q and A247S mutations in mKO2-hCdt1 will not affect plasmid function.
Proper citation: RRID:Addgene_206280 Copy
Species: Pseudomonas putida DOT-T1E
Genetic Insert: single-stranded DNA recombineering
Vector Backbone Description: Backbone Marker:SEVA collection; Backbone Size:5089; Vector Backbone:pSEVA624; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37798358
Proper citation: RRID:Addgene_199241 Copy
Species: Synthetic
Genetic Insert: CspRecT / Bxb-1
Vector Backbone Description: Vector Backbone:pKM461; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
Comments: Replicating ORBIT V2 helper plasmid w/ inducible oligo recombineering module (m-tol) and bxb-1 integrase (arabinose). SacB is present for counterselection and gentamicin is the resistance marker. V2 plasmid does not have mutL, which lowers background mutation rate. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint.
Proper citation: RRID:Addgene_205293 Copy
Species: Scytonema hofmannii
Genetic Insert: ShTnsB, ShTnsC, ShTniQ, ShCas12k
Vector Backbone Description: Backbone Size:3359; Vector Backbone:pR01600; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37368499
Comments: The vector backbone was derived from plasmid pFlpe2 from
Choi, K. H., Mima, T., Casart, Y., Rholl, D., Kumar, A., Beacham, I. R., & Schweizer, H. P. (2008). Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomallei. Applied and environmental microbiology, 74(4), 1064–1075. https://doi.org/10.1128/AEM.02430-07
Proper citation: RRID:Addgene_180076 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:2545; Vector Backbone:BASIC_SEVA_69.11; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:36381610
Proper citation: RRID:Addgene_194214 Copy
Vector Backbone Description: Backbone Size:57000; Vector Backbone:pTA-Mob 2.0; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology, Diatom Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37850145
Proper citation: RRID:Addgene_188609 Copy
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pTA-Mob 2.0; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37850145
Proper citation: RRID:Addgene_188610 Copy
Species: Homo sapiens
Genetic Insert: XRCC6
Vector Backbone Description: Backbone Marker:Addgene #25752; Vector Backbone:pEN_TTmiRC2-3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192302 Copy
Species: Homo sapiens
Genetic Insert: KPNB1
Vector Backbone Description: Backbone Marker:Addgene #25752; Vector Backbone:pEN_TTmiRC2-3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192301 Copy
Species: Homo sapiens
Genetic Insert: PFN2_variant2
Vector Backbone Description: Backbone Marker:Addgene #25752; Vector Backbone:pEN_TTmiRC2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192300 Copy
Species: Homo sapiens
Genetic Insert: KPNA2
Vector Backbone Description: Backbone Marker:Addgene #25752; Vector Backbone:pEN_TTmiRC2-3xFLAG-Linker; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192306 Copy
Species: Homo sapiens
Genetic Insert: KPNA1
Vector Backbone Description: Backbone Marker:Addgene #25752; Vector Backbone:pEN_TTmiRC2-3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192304 Copy
Species: Homo sapiens
Genetic Insert: TTI1
Vector Backbone Description: Backbone Marker:Addgene #25752; Vector Backbone:pEN_TTmiRC2-3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192303 Copy
Species: Other
Genetic Insert: Linker
Vector Backbone Description: Backbone Marker:Addgene #25752; Vector Backbone:pEN_TTmiRC2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192307 Copy
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