Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Genetic Insert: NA
Vector Backbone Description: Vector Backbone:NA; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:26829286
Comments: If LacI is added to the cell (e.g., in a plasmid), the expression of RFP will be somewhat repressed (unless if IPTG is added), as there is a lacO between the promoter and the gene's ATG. Attempts to remove this lacO were unsuccessful - we suspect the higher RFP expression might lead to cell death (in the cloning strain, E. coli DH5alpha).
Please note this strain has a higher %GC than E. coli, so PCRing (and sequencing) may be difficult.
Proper citation: RRID:Addgene_79642 Copy
Species: E.coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pbbr; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:16207908
Comments: Use gentamicin at 10 ug/mL.
Proper citation: RRID:Addgene_14467 Copy
Vector Backbone Description: Vector Backbone:pProUSER63H0G; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Proper citation: RRID:Addgene_191426 Copy
Genetic Insert: curT
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pBR322; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29357135
Comments: this plasmid has also been used in:
Heinz et al., Plant Cell 2016, DOI: https://doi.org/10.1105/tpc.16.00491
Proper citation: RRID:Addgene_107262 Copy
Species: Aequorea victoria (Jellyfish)
Genetic Insert: pLacZ-ECFP in pBBR1-mcs5
Vector Backbone Description: Backbone Size:800; Vector Backbone:pBBR1-mcs5; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:11059482
Comments: ECFP from Clontech
Proper citation: RRID:Addgene_107775 Copy
Species: Other
Genetic Insert: Spectinomycin resistance gene
Vector Backbone Description: Backbone Marker:Parniske lab; Backbone Size:2458; Vector Backbone:pUC57; Vector Types:Cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29077245
Proper citation: RRID:Addgene_104521 Copy
Species: Other
Genetic Insert: Tfd terminator
Vector Backbone Description: Backbone Marker:Parniske lab; Backbone Size:2458; Vector Backbone:pUC57; Vector Types:Cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29077245
Proper citation: RRID:Addgene_104520 Copy
Species: Other
Genetic Insert: Rsc202375 genomic upstream region
Vector Backbone Description: Backbone Marker:Parniske lab; Backbone Size:4190; Vector Backbone:LI+BpiI; Vector Types:Cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29077245
Proper citation: RRID:Addgene_104526 Copy
Species: Other
Genetic Insert: Kanamycin resistance gene
Vector Backbone Description: Backbone Marker:Parniske lab; Backbone Size:4190; Vector Backbone:LI+BpiI; Vector Types:Cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29077245
Proper citation: RRID:Addgene_104523 Copy
Species: Other
Genetic Insert: Chloramphenicol resistance gene
Vector Backbone Description: Backbone Marker:Parniske lab; Backbone Size:2458; Vector Backbone:pUC57; Vector Types:Cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29077245
Proper citation: RRID:Addgene_104525 Copy
Species: Other
Genetic Insert: Gala7 promoter
Vector Backbone Description: Backbone Marker:Parniske lab; Backbone Size:2458; Vector Backbone:pUC57; Vector Types:Cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29077245
Proper citation: RRID:Addgene_104516 Copy
Species: Other
Genetic Insert: hrpB promoter
Vector Backbone Description: Backbone Marker:Parniske lab; Backbone Size:2458; Vector Backbone:pUC57; Vector Types:Cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29077245
Proper citation: RRID:Addgene_104514 Copy
Species: S. pasteurianus
Genetic Insert: S. pasteurianus sgRNA
Vector Backbone Description: Backbone Size:5518; Vector Backbone:pBBR1; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29311279
Proper citation: RRID:Addgene_105232 Copy
Species: Other
Genetic Insert: Lifeact-Citrine
Vector Backbone Description: Vector Backbone:pDONR207; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27703856
Proper citation: RRID:Addgene_100590 Copy
Species: Mus musculus
Genetic Insert: mTalin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Repoter plasmid; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27703856
Proper citation: RRID:Addgene_100591 Copy
Species: Marchantia polymorpha
Genetic Insert: MpPHOT
Vector Backbone Description: Vector Backbone:pDONR207; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27027881
Proper citation: RRID:Addgene_100600 Copy
Species: bacteria
Genetic Insert: Promotorless gfp reporter gene
Vector Backbone Description: Vector Backbone:pVS1/p15a; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:11059491
Comments: Please refer to the attached table for a complete list of restriction sites in the MCS.
This plasmid has the XbaI site.
Proper citation: RRID:Addgene_37814 Copy
Species: Synthetic
Genetic Insert: Citrine-NLS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3374; Vector Backbone:pDONR207; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:23421791
Proper citation: RRID:Addgene_44586 Copy
Genetic Insert: Gentamycin
Vector Backbone Description: Backbone Marker:Bagdasarian Lab; Vector Backbone:pMMB67EH; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:10447882
Comments: This is a vector control for IPTG-indicible E. coli relA gene.
Proper citation: RRID:Addgene_45475 Copy
Genetic Insert: relA
Vector Backbone Description: Backbone Marker:Bagdasarian Lab; Vector Backbone:pMMB67EH; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:10447882
Comments: This is a vector control for IPTG-indicible E. coli relA gene.
Proper citation: RRID:Addgene_45474 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within dkNET that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
