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Genetic Insert: flaA-GFP
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pTLP6; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:10447882
Comments: The ~1kb BamHI/SphI flaAGFP fragment from pflaG into the ~4.6kb BamHI/SphI fragment of the ColE1 replicon pTLP6.
Proper citation: RRID:Addgene_45476 Copy
Species: S. pyogenes
Genetic Insert: tracrRNA
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23761437
Proper citation: RRID:Addgene_46570 Copy
Species: S. pyogenes
Genetic Insert: tracrRNA
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23761437
Proper citation: RRID:Addgene_46569 Copy
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:4980; Vector Backbone:pBR322; Vector Types:sequencing vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_46858 Copy
Species: Moraxella bovoculi
Genetic Insert: MbCas12a
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:2600; Vector Backbone:CmR/p15A; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30190307
Proper citation: RRID:Addgene_115654 Copy
Species: Arabidopsis thaliana
Genetic Insert: 4-coumaroyl CoA ligase 1
Vector Backbone Description: Backbone Size:4449; Vector Backbone:pAC; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:16551366
Comments: Initial gene cloning described in the following:
"Exploring Recombinant Flavonoid Biosynthesis in Metabolically Engineered Escherichia coli" (2004)Kevin T. Watts, Pyung Cheon Lee Dr., Claudia Schmidt-Dannert Prof. ChemBioChem. DOI: 10.1002/cbic.200300783
Proper citation: RRID:Addgene_35947 Copy
Genetic Insert: HEH-3
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36282 Copy
Genetic Insert: HE-6
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36279 Copy
Genetic Insert: HE-5
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36278 Copy
Genetic Insert: EH-4
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36273 Copy
Genetic Insert: EH-3
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36272 Copy
Genetic Insert: EH-2
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36271 Copy
Genetic Insert: E. coli KdtA
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36268 Copy
Vector Backbone Description: Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36267 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Strain description: CsrA system knockout
Chromosomal changes: ΔCsrA, ΔCsrB, ΔCsrC, ΔglgCAP, ΔpgaABCD, +LacIq at galK
Proper citation: RRID:Addgene_37980 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17220888
Comments: Host strain for pHL32B
Proper citation: RRID:Addgene_37976 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17220888
Comments: Host strain for pHL32B
Strain description: ΔDam
Chromosomal changes: ΔDam
Proper citation: RRID:Addgene_37977 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17220888
Comments: Host strain for pHL32B
Strain description: Δ(Dam+OxyR)
Chromosomal changes: Δ(Dam+OxyR)
Proper citation: RRID:Addgene_37979 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:2070; Vector Backbone:pPROLar.A122; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:19915540
Comments: MG1655 for expression
Proper citation: RRID:Addgene_34617 Copy
Species: Sperm Whale: Physeter macrocephalus
Genetic Insert: Myoglobin-127TAG
Vector Backbone Description: Vector Backbone:pSepT; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21868676
Comments: To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which is catalyzing the last step in serine biosynthesis, was deleted from Escherichia coli strains Top10 (Top10∆serB - Addgene #34928) and BL21 (BL21∆serB - Addgene #34929). These strains are required hosts when using this plasmid.
Proper citation: RRID:Addgene_34625 Copy
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