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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pGM1192 Resource Report Resource Website 1+ mentions |
RRID:Addgene_115683 | mCherry, aac(3)IV, tsr | Synthetic | Apramycin | Mobilizable, stable replicating expression vector for Streptomyces, strong constitutive SF14* promoter, codon optimized Strep-tagII-mCherry gene, tsr, aac(3)IV | Vector Backbone:pSG5; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin | mCherry codon optimized for Streptomyces | 2026-09-01 09:13:26 | 1 | |
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pGMGUS Resource Report Resource Website 1+ mentions |
RRID:Addgene_115678 | gusA, aac(3)IV, tsr | Streptomyces | Apramycin | Thermosensitive replication (< 37°C) in Streptomyces, Detection of single/double cross over by X-gluc colour reaction , aac(3)IV | Vector Backbone:pSG5; Vector Types:Gene knock out; Bacterial Resistance:Apramycin | 2026-09-01 09:13:26 | 2 | ||
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pACEC3GH (Apr) Resource Report Resource Website |
RRID:Addgene_110085 | Apramycin | PMID:29934571 | Vector Backbone:pACEC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin | 2026-09-01 09:11:51 | 0 | ||||
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pKO (Apr) Resource Report Resource Website 1+ mentions |
RRID:Addgene_110088 | Apramycin | PMID:29934571 | Vector Backbone:pKO; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Apramycin | 2026-09-01 09:11:51 | 1 | ||||
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pL97 Resource Report Resource Website |
RRID:Addgene_78576 | Apramycin | Vector Backbone:pIJ8668; Vector Types:Bacterial Expression, Streptomyces expression; Bacterial Resistance:Apramycin | 2026-09-01 10:02:29 | 0 | |||||
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pCRISPR-Cas9-ScaligD Resource Report Resource Website 1+ mentions |
RRID:Addgene_125688 | streptomyces codon optimized spCas9, sgRNA casette, and ScaligD | Other | Apramycin | PMID:25806970 | Please note that an IS1 transposase sequence was found downstream of Cas9 during Addgene's quality control. The depositor noted that this sequence should NOT affect plasmid function. | Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin | 2026-09-01 09:16:10 | 1 | |
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pCRISPR-dCas9 Resource Report Resource Website 1+ mentions |
RRID:Addgene_125687 | streptomyces codon optimized spdCas9, sgRNA casette | Other | Apramycin | PMID:25806970 | Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin | 2026-09-01 09:16:10 | 2 | ||
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p15a-AsCas12f-apmR Resource Report Resource Website 1+ mentions |
RRID:Addgene_171610 | AsCas12f1 | Other | Apramycin | PMID:34475565 | Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin | 2026-09-01 09:28:37 | 1 | ||
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pGM1202 Resource Report Resource Website 1+ mentions |
RRID:Addgene_69615 | aac | E. coli | Apramycin | PMID:30191290 | Backbone Size:2140; Vector Backbone:pSG5; Vector Types:; Bacterial Resistance:Apramycin | 2026-09-01 10:00:22 | 2 | ||
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pGM1190 Resource Report Resource Website 1+ mentions |
RRID:Addgene_69994 | aac | E. coli | Apramycin | PMID:30191290 | Backbone Size:2140; Vector Backbone:pSG5; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin | 2026-09-01 10:00:28 | 2 | ||
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pCRISPomyces-1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_61736 | sSpCas9 | Synthetic | Apramycin | PMID:25458909 | Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin | 2026-09-01 09:58:40 | 1 | ||
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pCRISPomyces-2 Resource Report Resource Website 10+ mentions |
RRID:Addgene_61737 | sSpCas9 | Synthetic | Apramycin | PMID:25458909 | Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin | 2026-09-01 09:58:40 | 19 | ||
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pKCcas9dO Resource Report Resource Website 1+ mentions |
RRID:Addgene_62552 | Scocas9 | artificially synthesized, codon optimized gene from Strep pyogenes | Apramycin | PMID:25739462 | More information is provided in the supplemental files. Please note that the sequence of the backbone (pKC1139) was obtained via Sanger sequencing and several regions may have discrepancies due to high GC content. These discrepancies have no functional consequences. | Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR, Bacterial Genome Editing; Bacterial Resistance:Apramycin | 2026-09-01 09:58:51 | 4 | |
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pYL-kasOp-FnCas12a3 Resource Report Resource Website |
RRID:Addgene_177875 | Streptomyces codon optimized the FnCas12a mutant EP16 | Streptomyces | Apramycin | PMID:32695773 | Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR; Bacterial Resistance:Apramycin | 2026-09-01 09:30:23 | 0 | ||
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pSelAct-KO Resource Report Resource Website |
RRID:Addgene_174374 | Apramycin | PMID:34890250 | Also known as pSelAct. For cloning in homology arms, can use Gateway, restriction-ligation, or homology-based cloning such as Gibson/Infusion cloning. Please cite at minimum paper 1) and a secondary paper that is most appropriate to your system of interest (2 or 3). 1. First development of pSelAct for knockouts in Rhodococcus equi: Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151 2. First development of pSelAct with Gateway cassette for knockouts in Rhodococcus fasicans: Savory, E. A., Weisberg, A. J., Stevens, D. M., Creason, A. L., Fuller, S. L., Pearce, E. M., and Chang, J. H. 2020. Phytopathogenic Rhodococcus Have Diverse Plasmids With Few Conserved Virulence Functions. Front Microbiol. 11:1022 3. Later referred to as pSelAct-KO after optimization for knockouts in Clavibacter: Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345 | Backbone Size:7373; Vector Backbone:pUC19; Vector Types:Vector for 2-step knockout by homologous recombination; Bacterial Resistance:Apramycin | 2026-09-01 09:29:24 | 0 | |||
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pOSV801 Resource Report Resource Website |
RRID:Addgene_126044 | Apramycin | PMID:31175189 | Backbone Size:5613; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Apramycin | 2026-09-01 09:16:17 | 0 | ||||
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pOSV802 Resource Report Resource Website |
RRID:Addgene_126595 | Apramycin | PMID:31175189 | Backbone Size:5681; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Apramycin | 2026-09-01 09:16:27 | 0 | ||||
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pOSV803 Resource Report Resource Website |
RRID:Addgene_126596 | Apramycin | PMID:31175189 | Backbone Size:5220; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Apramycin | 2026-09-01 09:16:27 | 0 | ||||
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pMS17 Resource Report Resource Website |
RRID:Addgene_127088 | tcp830 | Synthetic | Apramycin | PMID:15917435 | For research using this plasmid, please acknowledge the depositing laboratory and associated citation. | Backbone Size:4460; Vector Backbone:pSET152; Vector Types:Streptomyce integration vector derived from phiC31 int/attP; Bacterial Resistance:Apramycin | 2026-09-01 09:16:36 | 0 | |
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pIB1884-ximD-PCPT Resource Report Resource Website |
RRID:Addgene_169109 | XimD and PcPT | Synthetic | Apramycin | PMID:32786357 | Vector Backbone:pSE152; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin | 2026-09-01 09:27:57 | 0 |
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