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Species: Rattus norvegicus
Genetic Insert: Col2.3 promoter + deltaTK + BGH polyA
Vector Backbone Description: Backbone Size:1850; Vector Backbone:pBR327; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11760835
Proper citation: RRID:Addgene_114273 Copy
Species: Rattus norvegicus
Genetic Insert: Erythropoietin
Vector Backbone Description: Vector Backbone:pCLXEBR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114285 Copy
Species: Rattus norvegicus
Genetic Insert: Erythropoietin Receptor
Vector Backbone Description: Vector Backbone:pCWXPGR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114286 Copy
Species: Rattus norvegicus
Genetic Insert: Erythropoietin
Vector Backbone Description: Vector Backbone:pCWXPGR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114290 Copy
Species: Rattus norvegicus
Genetic Insert: IRS-3
Vector Backbone Description: Backbone Marker:pBABE-hygro is available at Addgene; Backbone Size:5558; Vector Backbone:pBABE hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11113178
Comments: Rat IRS-3 was generated by PCR from the rat genomic DNA sequence. The intron was removed by PCR overlap extension using the following two pairs of primers: 5'-CGTGGATCCGCGATGAAGCCTGCAGGTACG-3' (sense) plus 5'-CTTGGGGGCTGAAACCCATGTTTGCTGGGCA-3' (antisense) and 5'-TGCCCAGCAAACATGGGTTTCAGCCCCCAAG-3' (sense) plus 5'-GCTGTCGACGTTCTAGAACTTGATGCTG-3' (antisense). BamHI and SalI sites were introduced at the 5' and 3' ends, respectively, of the coding sequence of IRS-3 by PCR. The subsequently generated coding sequence of rat IRS-3 was ligated into BamHI and SalI sites in pBABE-hygro.
Proper citation: RRID:Addgene_11450 Copy
Species: Rattus norvegicus
Genetic Insert: 2xPH(PLCdelta) fusion
Vector Backbone Description: Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11854411
Comments: Please note that this plasmid contains aa11-140 when compared to GenBank reference sequence NP_058731.1. According to this reference sequence the PH domain is aa17-128. The additional residues surrounding the PH domain were intentionally cloned into this construct.
Proper citation: RRID:Addgene_36092 Copy
Species: Rattus norvegicus
Genetic Insert: G protein subunit Gamma 5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16517125
Proper citation: RRID:Addgene_36044 Copy
Species: Rattus norvegicus
Genetic Insert: protein tyrosine phosphatase 1B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18332219
Comments: A Kozak methionine initiator sequence and a Flag tag were added to the N terminus of rat PTP1B, which was amplified by PCR with the following primers: 5′-CTCGGATCCGCCACCATGGACTACAAGGACGACGATGACAAGAAGCTTGGGATGGAAATGGAGAAGGAATTCGAG-3′ and 5′-CTCTAGACTCGAGCGGCCGCTCAGTGAAAACATACCCGGTAACAG-3′. The PCR product was digested with BamHI and NotI and subsequently ligated into the corresponding sites of pCDNA3.1(+) (Invitrogen)
Please note that Addgene's sequencing results differ from the full plasmid sequence information from the depositing laboratory at bp# 889-910, indicating that the NheI and AflII sites are no longer present in the plasmid.
Proper citation: RRID:Addgene_35988 Copy
Species: Rattus norvegicus
Genetic Insert: protein tyrosine phosphatase 1B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4407; Vector Backbone:pTrcHis A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18332219
Proper citation: RRID:Addgene_35987 Copy
Species: Rattus norvegicus
Genetic Insert: CD200R
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18296487
Proper citation: RRID:Addgene_36153 Copy
Species: Rattus norvegicus
Genetic Insert: G protein alpha 0
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7261; Vector Backbone:pd12.2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15078878
Comments: The CFP was introduced between 92nd and 93rd amino acid of alpha-0
Proper citation: RRID:Addgene_36312 Copy
Species: Rattus norvegicus
Genetic Insert: beta-arrestin2
Vector Backbone Description: Backbone Size:5015; Vector Backbone:pcDNA3.1 zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16687412
Proper citation: RRID:Addgene_36917 Copy
Species: Rattus norvegicus
Genetic Insert: beta-arrestin1
Vector Backbone Description: Backbone Size:5015; Vector Backbone:pcDNA3.1 zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16687412
Proper citation: RRID:Addgene_36916 Copy
Species: Rattus norvegicus
Genetic Insert: SypHluorin 4x
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19217377
Proper citation: RRID:Addgene_37005 Copy
Species: Rattus norvegicus
Genetic Insert: CaM Kinase II
Vector Backbone Description: Backbone Marker:Maurer lab; Backbone Size:4265; Vector Backbone:pRSV-link; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7958915
Proper citation: RRID:Addgene_45064 Copy
Species: Rattus norvegicus
Genetic Insert: Kif5c
Vector Backbone Description: Backbone Size:6755; Vector Backbone:pBa-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16543128
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.
Proper citation: RRID:Addgene_45059 Copy
Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11060023
Proper citation: RRID:Addgene_45244 Copy
Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11060023
Proper citation: RRID:Addgene_45245 Copy
Species: Rattus norvegicus
Genetic Insert: PKA regulatory subunit RII beta
Vector Backbone Description: Backbone Marker:Karel Svoboda; Backbone Size:5900; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, with chicken beta actin promoter and WPRE; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19447092
Proper citation: RRID:Addgene_45529 Copy
Species: Rattus norvegicus
Genetic Insert: NR1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12160751
Proper citation: RRID:Addgene_45446 Copy
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