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Species: Caenorhabditis elegans
Genetic Insert: DAF-16a
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:6000; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24671950
Proper citation: RRID:Addgene_175283 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16a mini-gene
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSL1180; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24671950
Proper citation: RRID:Addgene_175284 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-3::mNeonGreen
Vector Backbone Description: Backbone Size:2445; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37396790
Comments: C. elegans codon-optimized mNeonGreen. Use as an array marker for CRISPR or as a general co-injection marker. Very bright mNG fluorescence in body wall muscle. Inject at 5 ng/uL. Made by George Huang (Glow Worms '20).
pGLOW79 has 10 random mutations relative to the pCFJ104 backbone that do not affect plasmid function.
Proper citation: RRID:Addgene_177339 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-2::mNeonGreen
Vector Backbone Description: Backbone Size:2445; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37396790
Comments: C. elegans codon-optimized mNeonGreen. Use as an array marker for CRISPR or as a general co-injection marker. Very bright mNG fluorescence in pharyngeal muscle. Inject at 2.5 ng/uL. Made by George Huang (Glow Worms '20).
pGLOW77 has 11 random mutations relative to the pCFJ90 backbone that do not affect plasmid function.
Proper citation: RRID:Addgene_177338 Copy
Species: Caenorhabditis elegans
Genetic Insert: cye-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21392 Copy
Species: Caenorhabditis elegans
Genetic Insert: L3787 promoter::GFP in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21377 Copy
Species: Caenorhabditis elegans
Genetic Insert: spe-11 promoter (no ATG) in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21385 Copy
Species: Caenorhabditis elegans
Genetic Insert: pie-1 promoter (short, no ATG) in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21384 Copy
Species: Caenorhabditis elegans
Genetic Insert: gld-1 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21389 Copy
Species: Caenorhabditis elegans
Genetic Insert: msp-56 promoter in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21383 Copy
Species: Caenorhabditis elegans
Genetic Insert: him-3 prom in PDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:PDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21382 Copy
Species: Caenorhabditis elegans
Genetic Insert: lip-1 M1M2 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21381 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119
Vector Backbone Description: Backbone Marker:Epicentre; Backbone Size:300; Vector Backbone:R6K origin PCR fragment; Vector Types:Worm Expression, Adds unc-119 marker to Amp resistant plasmids; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19520111
Comments: This plasmid has small deletions within the KanR gene. We generated these fragments by PCR so it is definitely possible that these were generated during amplification, these changes must not impair function as the construct is Kan resistant both as a plasmid and following recombination.
Proper citation: RRID:Addgene_21595 Copy
Species: Caenorhabditis elegans
Genetic Insert: (Gly)5Ala::citrine(w introns)::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21511 Copy
Species: Caenorhabditis elegans
Genetic Insert: (Gly)5Ala::GFP(w introns)::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21509 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-16
Vector Backbone Description: Backbone Marker:Andrew Fire (Addgene Plasmid 1497); Backbone Size:4406; Vector Backbone:pPD95.81; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23805378
Comments: The D->V point mutation in DAF-16 has no functional consequence.
Proper citation: RRID:Addgene_52032 Copy
Species: Caenorhabditis elegans
Genetic Insert: fli-1
Vector Backbone Description: Backbone Marker:Fire lab; Backbone Size:4487; Vector Backbone:pPD95.77; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18485202
Proper citation: RRID:Addgene_52348 Copy
Species: Caenorhabditis elegans
Genetic Insert: swan-1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6299; Vector Backbone:pCMV-3xFLAG; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16980389
Proper citation: RRID:Addgene_52349 Copy
Species: Caenorhabditis elegans
Genetic Insert: rack-1
Vector Backbone Description: Backbone Marker:Fire lab; Backbone Size:4487; Vector Backbone:pPD95.77; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21124943
Proper citation: RRID:Addgene_52351 Copy
Species: Caenorhabditis elegans
Genetic Insert: Dre-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5404; Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24613396
Proper citation: RRID:Addgene_52517 Copy
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