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Species: E. coli
Genetic Insert: RuvC
Vector Backbone Description: Backbone Marker:made by S Tabor/sold by USB; Backbone Size:2743; Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8106500
Proper citation: RRID:Addgene_20169 Copy
Species: E. coli
Genetic Insert: DHFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Comments: murine VH chain signal peptide followed by a HA
tag and one repeat of DHFR
Proper citation: RRID:Addgene_20217 Copy
Species: E. coli
Genetic Insert: 3xDHFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Proper citation: RRID:Addgene_20216 Copy
Species: E. coli
Genetic Insert: DHFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Proper citation: RRID:Addgene_20214 Copy
Species: E. coli
Genetic Insert: Pmyc1tetO-lacZ
Vector Backbone Description: Backbone Size:4103; Vector Backbone:pTC-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19174563
Proper citation: RRID:Addgene_20315 Copy
Species: E. Coli
Genetic Insert: AP-CFP-TM
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15897449
Comments: Please email [email protected] with intended use and/or targets for this plasmid
Proper citation: RRID:Addgene_20861 Copy
Species: E. Coli
Genetic Insert: Streptavidin-Alive
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16554831
Comments: Please email [email protected] with your intended use or targets for this plasmid.
Proper citation: RRID:Addgene_20860 Copy
Species: E. Coli
Genetic Insert: Streptavidin-Dead
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16554831
Comments: Please email [email protected] with intended use and/or targets for this plasmid.
Proper citation: RRID:Addgene_20859 Copy
Species: E. coli
Genetic Insert: BirA
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15897449
Comments: Please email [email protected] with your intended use and/or targets for this plasmid.
Proper citation: RRID:Addgene_20857 Copy
Species: E. coli
Genetic Insert: LacZ
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJH271; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1545810
Comments: The HindIII fragment of pJF3 containing URA3 UAScycl- and 300 bp of the LEU2 gene promoter and
LacZ::CS cut site was inserted into the HindIII site at the
upstream end of a promotorless LacZ gene on plasmid JH271.
The two LacZ sequences are in direct repeat orientation relative to each other.
The lambda sequences are of unknown origin
Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.
Proper citation: RRID:Addgene_21678 Copy
Species: E. coli
Genetic Insert: Chloramphenicol resistance gene
Vector Backbone Description: Vector Backbone:pCas; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29884781
Proper citation: RRID:Addgene_113633 Copy
Species: E. coli
Genetic Insert: tdNfsB(F124W)-mCherry
Vector Backbone Description: Backbone Marker:Scott Sternson; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30111097
Comments: Sternson Lab recommendations for propagation of AAV-FLEX plasmids: "These vectors are prone to recombination. This is a well known issue with these AAV vectors and is due to the inverted terminal repeats (ITRs) required for AAV production. To minimize recombination, we propagate these plasmids in NEB Stable cells. Also, to minimize recombination, cells should be cultured at 30 C.
Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended.
Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed."
Proper citation: RRID:Addgene_113762 Copy
Species: E. coli
Genetic Insert: clbO
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114153 Copy
Species: E. coli
Genetic Insert: clbK
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114152 Copy
Species: E. coli
Genetic Insert: clbH
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28805802
Proper citation: RRID:Addgene_114157 Copy
Species: E. coli
Genetic Insert: clbB
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28805802
Proper citation: RRID:Addgene_114155 Copy
Species: E. coli
Genetic Insert: ClbD
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114146 Copy
Species: E. coli
Genetic Insert: ClbF
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114148 Copy
Species: E. coli
Genetic Insert: ClbE
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114147 Copy
Species: E. coli
Genetic Insert: OsmC
Vector Backbone Description: Backbone Size:5386; Vector Backbone:pSKB3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15103136
Comments: Please note that the plasmid pSKB3.EG10680 contains a Kanamycin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
Proper citation: RRID:Addgene_11436 Copy
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