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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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PSOP1-COMP-blac-flag-his Resource Report Resource Website |
RRID:Addgene_111003 | secreted ookinete protein (PSOP1) | Synthetic | Ampicillin | PMID:33515807 | Backbone Marker:pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Backbone Size:7661; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | All NXT/S sites mutated to NXA, where X is any amino acid except Proline | 2026-07-25 12:35:04 | 0 | |
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CPN60-COMP-blac-flag-his Resource Report Resource Website |
RRID:Addgene_111004 | CPN60 | Synthetic | Ampicillin | PMID:33515807 | Backbone Marker:pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Backbone Size:7661; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | All NXT/S sites mutated to NXA, where X is any amino acid except Proline | 2026-07-25 12:35:04 | 0 | |
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AAP-COMP-blac-flag-his Resource Report Resource Website |
RRID:Addgene_111001 | aminopeptidase P (APP) | Synthetic | Ampicillin | PMID:33515807 | Backbone Marker:pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Backbone Size:7661; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | All NXT/S sites mutated to NXA, where X is any amino acid except Proline | 2026-07-25 12:35:04 | 0 | |
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pRK5-HA ZNHIT3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_110953 | ZNHIT3 | Homo sapiens | Ampicillin | PMID:29700228 | Backbone Size:5000; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-07-25 12:35:04 | 1 | ||
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pLJM60-FLAG NUFIP1 Resource Report Resource Website |
RRID:Addgene_110950 | NUFIP1 | Homo sapiens | Ampicillin | PMID:29700228 | Backbone Size:11000; Vector Backbone:pLJM60; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:35:04 | 0 | ||
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pLJM60 FLAG NUFIP1 W40A Resource Report Resource Website |
RRID:Addgene_110951 | NUFIP1 W40A | Homo sapiens | Ampicillin | PMID:29700228 | Vector Backbone:pLJM60; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | changed Tryptophan 40 to alanine | 2026-07-25 12:35:04 | 0 | |
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PF3D7_0910300-COMP-blac-flag-his Resource Report Resource Website |
RRID:Addgene_110959 | conserved Plasmodium protein, unknown function | Synthetic | Ampicillin | PMID:33515807 | Backbone Marker:pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Backbone Size:7661; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | All NXT/S sites mutated to NXA, where X is any amino acid except Proline | 2026-07-25 12:35:04 | 0 | |
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piHGpd/T22I Resource Report Resource Website |
RRID:Addgene_1110 | HSP82 | Saccharomyces cerevisiae | Ampicillin | PMID:9990037 | Backbone Marker:ATCC (#77189); Backbone Size:4453; Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | HSP82 with threonine 22 changed to isoleucine; regulated by the strong constitutive promoter GPD | 2026-07-25 12:35:04 | 0 | |
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WT NFAT1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_11100 | NFAT1 | Mus musculus | Ampicillin | PMID:12355451 | Backbone Marker:Genetics Institute; Backbone Size:0; Vector Backbone:GFP-RV-DV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:35:04 | 9 | ||
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pLKO-puro-tetON-shOGDH 1714 Resource Report Resource Website |
RRID:Addgene_110941 | shOGDH | Homo sapiens | Ampicillin | PMID:28396387 | Recombination can be minimized by using NEB Stable E. coli strain grown at 30C to amplify the plasmid DNA. Even so, low recombination rate may still happen and when you eventually amplify the DNA you may need to screen through several colonies and perform diagnostic digests to identify the correct ones. | Backbone Marker:Dmitri Wiederschain; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:RNAi; Bacterial Resistance:Ampicillin | 2026-07-25 12:35:04 | 0 | |
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pLKO-puro-tetON-shRFP Resource Report Resource Website |
RRID:Addgene_110940 | shRFP | Ampicillin | PMID:28396387 | Recombination can be minimized by using NEB Stable E. coli strain grown at 30C to amplify the plasmid DNA. Even so, low recombination rate may still happen and when you eventually amplify the DNA you may need to screen through several colonies and perform diagnostic digests to identify the correct ones. | Backbone Marker:Dmitri Wiederschain; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin | 2026-07-25 12:35:04 | 0 | ||
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pmTurquoise-parkin Resource Report Resource Website |
RRID:Addgene_110945 | E3 ubiquitin ligase parkin | Homo sapiens | Kanamycin | PMID:33634293 | Backbone Size:4698; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-07-25 12:35:04 | 0 | ||
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pmTurquoise2-Drp1 Resource Report Resource Website |
RRID:Addgene_110946 | Dynamin-1-like protein | Homo sapiens | Kanamycin | Backbone Marker:Gia Voeltz lab; Backbone Size:3833; Vector Backbone:mCh-Drp1 (Plasmid #49152); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-07-25 12:35:04 | 0 | |||
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pANY1 Resource Report Resource Website |
RRID:Addgene_110949 | ccd B cassette | Synthetic | Kanamycin | PMID:29448102 | During molecular cloning, DNA of interest can be inserted into pANY1 using both MCS-1 and MCS-2, with deletion of the ccdB cassette. Thus the recombinant plasmid can be transformed into commonly used E. coli strains (e.g., JM109, BL21, DH5a). Because the original plasmid, pANY1, cannot be transformed into these E. coli strains, false positives can be eliminated. | Backbone Marker:Yingfeng An; Vector Backbone:pANY1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-07-25 12:35:04 | 0 | |
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pmTurquoise2-LAMP1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_110948 | Lysosomal-associated membrane protein 1 | Homo sapiens | Ampicillin | PMID:30874551 | Backbone Size:5400; Vector Backbone:pAc5.1/V5-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-07-25 12:35:04 | 1 | ||
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KG#470 Resource Report Resource Website |
RRID:Addgene_110930 | rab-3 promoter | Caenorhabditis elegans | Ampicillin | PMID:15489510 | Unique multi-cloning sites: Nhe I, Sbf I, Kpn I/ Age I/ Xho I, Bgl II This vector is the same as KG#59, except the Sal I and Acc I sites of the multi-cloning site have been replaced with a Sbf I site. Synthesized 2 complementary oligos containing Nhe I and Age I sticky ends and Sbf I and Kpn I sites in between. Hybridization of the 2 oligos to each other will produce Nhe I and Age I sticky ends. After hybridization, this synthetic insert (which is non-phosphorylated on its 5' termini) was cloned into Nhe I/ Age I cut KG#59. Transformed into XL1-Blue electrocompetent cells. Miniprepped 6 clones and tested for the Sbf I site by cutting with Sbf I. Chose one clone that has the Sbf I site with correct band size (and only a single band) and made the glycerol stock. Features of the expression construct: This expression construct has a promoter (rab-3) that will drive strong and specific expression (of whatever gene is inserted in the MCS) in the entire nervous system of juvenile and adult animals. The boundaries of the rab-3 promoter sequence were obtained by examining pRabGFPrim3' from Mike Nonet's lab web site and comparing that to the genome sequence. Other features of the expression vector include a "decoy" 5' to the promoter to reduce non-specific expression in the gut (see Fire Lab 1995 Kit documentation for description), the unc-54 3' end including the poly A addition signal, and 3 introns (1 in 5' UTR, 1 just upstream of the unc-54 3' end, and 1 inserted in the unc-54 3' end). These introns help provide more uniform and robust expression, especially if you are expressing a cDNA with no introns as opposed to a gene with introns. Note: this vector can be converted to a vector that fuses GFP, YFP, or CFP onto the C-terminus of the protein as follows: Remove the ~1000 bp (Kpn I or Age I) + (Spe I or BsiW I [expensive, 55 C cutter with 50% activity at 37 C; heat inactivate 80 C] or Apa I) fragment containing the unc-54 3’ control region from this plasmid and replace it with the ~1800 bp like-digested fragment containing 3-intron S65C GFP (see pPD94.81 in Fire Lab plasmids), YFP (see pPD136.64 in Fire Lab plasmids) or CFP (see pPD136.61 in Fire Lab plasmids) + the unc-54 3’ UTR and control region. Note if Spe I is used to cut these Fire Lab vectors, you get 3 bands: 1100, 1800, and 4000 (total size 6.9 Kb). The 1800 bp band is the XFP-containing band. For C-terminal fusions, remember to leave the stop codon off of the upstream protein. For the above-mentioned Fire Lab vectors, the reading frame for the downstream XFP relative to the Kpn I and Age I sites is as follows (triplets are the reading frame codons): Kpn I G GTA CCG GT Age I Alternatively, use Gibson Assembly/ NEBuilder to insert any genetically encoded fluorescent protein at the N- or C-terminus of your protein. | Backbone Size:2632; Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-07-25 12:35:03 | 0 | |
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KG#94 Resource Report Resource Website |
RRID:Addgene_110931 | unc-17 promoter | Caenorhabditis elegans | Ampicillin | PMID:17942708 | Unique multi-cloning sites: Nhe I, Sal I, Kpn I/ Age I/ Eco RV, Bgl II Used Apa I/ Msc I to cut out the ~1900 bp GFP/ 3' control region from RM#349p, leaving the 5900 bp vector fragment containing the unc-17 full promoter + remaining vector sequences. To this vector fragment, we ligated the 1079 bp Apa I/ Msc I fragment cut from pPD96.52 (C. elegans body wall muscle expression vector). Picked 6 colonies for minipreps. Chose 1 clone with correct size insert and made glycerol stock. Features of the expression construct: This is a C. elegans expression vector with the unc-17 3.2 Kb promoter + a large MCS for cloning in cDNAs. Includes cha-1/unc-17 upstream region from Sna-1 (=Bst1107 I = GTA/TAC) down to first half of the 66 bp first exon (which is common to unc-17 and cha-1 transcripts but is not translated). Includes beta site so that expression occurs in most cholinergic cells. Expression still missing from VC's and some cholinergic head and tail neurons. In this construct the GFP/ 3' control region of RM#349p is replaced with the MCS II + 3' control region of pPD96.52. This includes the unc-54 3' end including the poly A addition signal, and 3 introns (1 in 5' UTR, 1 just upstream of the unc-54 3' end, and 1 inserted in the unc-54 3' end). These introns help provide more uniform and robust expression, especially if you are expressing a cDNA with no introns as opposed to a gene with introns. Note: this vector can be converted to a vector that fuses GFP, YFP, or CFP onto the C-terminus of the protein as follows: Remove the ~1000 bp (Kpn I or Age I) + (Spe I or BsiW I [expensive, 55 C cutter with 50% activity at 37 C; heat inactivate 80 C] or Apa I) fragment containing the unc-54 3’ control region from this plasmid and replace it with the ~1800 bp like-digested fragment containing 3-intron S65C GFP (see pPD94.81 in Fire Lab plasmids), YFP (see pPD136.64 in Fire Lab plasmids) or CFP (see pPD136.61 in Fire Lab plasmids) + the unc-54 3’ UTR and control region. Note if Spe I is used to cut these Fire Lab vectors, you get 3 bands: 1100, 1800, and 4000 (total size 6.9 Kb). The 1800 bp band is the XFP-containing band. For C-terminal fusions, remember to leave the stop codon off of the upstream protein. For the above-mentioned Fire Lab vectors, the reading frame for the downstream XFP relative to the Kpn I and Age I sites is as follows (triplets are the reading frame codons): Kpn I G GTA CCG GT Age I Alternatively, use Gibson Assembly/ NEBuilder to insert any genetically encoded fluorescent protein at the N- or C-terminus of your protein. | Backbone Size:2632; Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-07-25 12:35:03 | 0 | |
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KG#493 Resource Report Resource Website |
RRID:Addgene_110932 | unc-17 promoter | Caenorhabditis elegans | Ampicillin | PMID:17942708 | Unique multi-cloning sites: Nhe I, Sbf I, Kpn I/ Age I/ Eco RV, Bgl II Note: this vector is the same as KG#94, except a Sbf I site replaces the Sal I site. Synthesized 2 complementary oligos containing Nhe I and Age I sticky ends and Sbf I and Kpn I sites in between. Hybridization of the 2 oligos to each other should produce Nhe I and Age I sticky ends. After hybridization, this synthetic insert (which is non-phosphorylated on its 5' termini) was cloned into Nhe I/ Age I cut KG#94. Transformed into XL1-Blue electrocompetent cells. Miniprepped 4 clones and tested for the Sbf I site by cutting with Sbf I. Chose one clone that has the Sbf I site with correct band size (and only a single band) and made a glycerol stock. Features of the expression construct: This is a C. elegans expression vector with the unc-17 3.2 Kb promoter + a large MCS for cloning in cDNAs. Includes cha-1/unc-17 upstream region from Sna-1 (=Bst1107 I = GTA/TAC) down to first half of the 66 bp first exon (which is common to unc-17 and cha-1 transcripts but is not translated). Includes beta site so that expression occurs in most cholinergic cells. Expression still missing from VC's and some cholinergic head and tail neurons. In this construct the GFP/ 3' control region of RM#349p is replaced with the MCS II + 3' control region of pPD96.52. This includes the unc-54 3' end including the poly A addition signal, and 3 introns (1 in 5' UTR, 1 just upstream of the unc-54 3' end, and 1 inserted in the unc-54 3' end). These introns help provide more uniform and robust expression, especially if you are expressing a cDNA with no introns as opposed to a gene with introns. Note: this vector can be converted to a vector that fuses GFP, YFP, or CFP onto the C-terminus of the protein as follows: Remove the ~1000 bp (Kpn I or Age I) + (Spe I or BsiW I [expensive, 55 C cutter with 50% activity at 37 C; heat inactivate 80 C] or Apa I) fragment containing the unc-54 3’ control region from this plasmid and replace it with the ~1800 bp like-digested fragment containing 3-intron S65C GFP (see pPD94.81 in Fire Lab plasmids), YFP (see pPD136.64 in Fire Lab plasmids) or CFP (see pPD136.61 in Fire Lab plasmids) + the unc-54 3’ UTR and control region. Note if Spe I is used to cut these Fire Lab vectors, you get 3 bands: 1100, 1800, and 4000 (total size 6.9 Kb). The 1800 bp band is the XFP-containing band. For C-terminal fusions, remember to leave the stop codon off of the upstream protein. For the above-mentioned Fire Lab vectors, the reading frame for the downstream XFP relative to the Kpn I and Age I sites is as follows (triplets are the reading frame codons): Kpn I G GTA CCG GT Age I Alternatively, use Gibson Assembly/ NEBuilder to insert any genetically encoded fluorescent protein at the N- or C-terminus of your protein. | Backbone Size:2632; Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-07-25 12:35:03 | 0 | |
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pLenti6.3-OGDH_wobble Resource Report Resource Website |
RRID:Addgene_110938 | OGDH wobble cDNA | Homo sapiens | Ampicillin | PMID:28396387 | Backbone Marker:Invitrogen; Backbone Size:9387; Vector Backbone:pLenti6.3/V5-DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 15 silent wobble mutations corresponding to three shRNA sites | 2026-07-25 12:35:03 | 0 | |
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pLKO-puro-tetON-shGFP Resource Report Resource Website |
RRID:Addgene_110939 | shGFP | Ampicillin | PMID:28396387 | Recombination can be minimized by using NEB Stable E. coli strain grown at 30C to amplify the plasmid DNA. Even so, low recombination rate may still happen and when you eventually amplify the DNA you may need to screen through several colonies and perform diagnostic digests to identify the correct ones. | Backbone Marker:Dmitri Wiederschain; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin | 2026-07-25 12:35:04 | 0 |
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