Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Synthetic
Genetic Insert: eYFP and Blasticigin resistance cassette
Vector Backbone Description: Backbone Marker:Thermor Fisher / Fermentas; Backbone Size:2972; Vector Backbone:pJet1.2; Vector Types:blunt cloning kit; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52559 Copy
Species: Homo sapiens
Genetic Insert: Fast-GCaMP-EF05
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:7831; Vector Backbone:pJFRC-MUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52558 Copy
Species: Homo sapiens
Genetic Insert: HMGCLL1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52434 Copy
Species: Drosophila melanogaster
Genetic Insert: VGlut-Gal80
Vector Backbone Description: Backbone Size:9052; Vector Backbone:pBPGAL80Uw-6; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene's quality control sequencing has found a number of discrepancies with the provided full plasmid sequence. These differences are not thought to affect plasmid function.
Proper citation: RRID:Addgene_52555 Copy
Species: Bacterial origin
Genetic Insert: Kanamycin cassette
Vector Backbone Description: Backbone Size:7145; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: This is a second generation plasmid that increases the efficiency of sgRNA guide cloning over the original "pLKO.1-puro U6 sgRNA BspMI stuffer" vector.
"pLKO.1-puro U6 sgRNA BfuAI large stuffer" was created to simplify the cloning of the sgRNA guide cassette into pLKO.1-puro U6 sgRNA BspMI stuffer vector originally described in the manuscript “Kearns, NA, etal. Development 2014, 141(1):219-23”. The original vector has a small intervening sequence that presents two problems: 1) the efficiency of excision by BfuAI is moderate, and 2) the size of this spacer is similar to the guide sequence, which makes distinguishing positive clones from reclosures challenging. Consequently, we have inserted a Kanamycin cassette between these sites at a unique Bcl1 site. This element is more efficiently excised by BfuAI and makes distinguishing vectors containing the desired guide sequence easy to distinguish from the parent vector. The excision of the Kan cassette by BfuAI cuts this cassette into 3 fragments due to two internal BfuAI sites within the Kan cassette. Otherwise the cloning of the guides is carried out as described in the manuscript.
Proper citation: RRID:Addgene_52628 Copy
Species: Rattus norvegicus
Genetic Insert: GLUT4
Vector Backbone Description: Backbone Marker:Bogan lab, unpublished; Backbone Size:5783; Vector Backbone:pB; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid is also called pB-G7. We use FACS to select infected cells that express GFP. There is a short linker between GLUT4 and GFP.
Proper citation: RRID:Addgene_52872 Copy
Species: Homo sapiens
Genetic Insert: HPV8 L1
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6295; Vector Backbone:pVITRO1-neo-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_52598 Copy
Species: Homo sapiens
Genetic Insert: HPV43 L1
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6295; Vector Backbone:pVITRO1-neo-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_52596 Copy
Species: Synthetic
Genetic Insert: Right AAVS1 TALEN
Vector Backbone Description: Vector Backbone:pZT; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Kanamycin
References:
Comments: pZT-AAVS1-R1 TALEN was assembled using RVD monomers from Golden Gate TALEN kit 1.0 (TALEN Kit #1000000016) and a mammalian TALEN expression vector pZT.
Proper citation: RRID:Addgene_52638 Copy
Species: Rattus norvegicus
Genetic Insert: Kir4.1
Vector Backbone Description: Vector Backbone:pZac 2.1; Vector Types:Mammalian Expression, AAV, Adeno-Associated Virus (); Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52874 Copy
Species:
Genetic Insert: hEµMAR-SFFV; eGFP
Vector Backbone Description: Backbone Size:6724; Vector Backbone:LeGO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52633 Copy
Species: Homo sapiens
Genetic Insert: Fast-GCaMP-EF15
Vector Backbone Description: Backbone Marker:Millipore; Backbone Size:5369; Vector Backbone:pET28b; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_52641 Copy
Species: Homo sapiens
Genetic Insert: Fast-GCaMP-EF09
Vector Backbone Description: Backbone Marker:Millipore; Backbone Size:5369; Vector Backbone:pET28b; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_52640 Copy
Species: Homo sapiens
Genetic Insert: Fast-GCaMP-RS09
Vector Backbone Description: Backbone Marker:Millipore; Backbone Size:5369; Vector Backbone:pET28b; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_52649 Copy
Species: Homo sapiens
Genetic Insert: Fast-GCaMP-RS06
Vector Backbone Description: Backbone Marker:Millipore; Backbone Size:5369; Vector Backbone:pET28b; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_52647 Copy
Species: Discosoma
Genetic Insert: DsRed1-E5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3959; Vector Backbone:pDsRed2-Mito; Vector Types:DsRed; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_52659 Copy
Species: Synthetic
Genetic Insert: TEM-1 β-Lactamase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_52656 Copy
Species:
Genetic Insert: KI (37)-Oplac2 strain
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
References:
Comments: To test whether the mRNA secondary structure of our codon redesigned sequences affected expression, we substituted the first 37 nucleotides of Oplac2 with those of KIlac, producing and KI(37)-Oplac2.
Proper citation: RRID:Addgene_52704 Copy
Species:
Genetic Insert: degtag strain
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
References:
Comments: To generate the degtag strain, we introduced the ssrA tag to the C-terminus of LacZ, thus targeting the protein for degradation by the ClpXP and ClpAP proteases.
Proper citation: RRID:Addgene_52705 Copy
Species:
Genetic Insert: KI (37)-Oplac1 strain
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
References:
Comments: To test whether the mRNA secondary structure of our codon redesigned sequences affected expression, we substituted the first 37 nucleotides of OpLac1 with those of KIlac.
Proper citation: RRID:Addgene_52703 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within dkNET that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
