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Species: Other
Genetic Insert: Contains Cas9
Vector Backbone Description: Vector Backbone:unknown; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26698661
Proper citation: RRID:Addgene_134247 Copy
Species: Mus musculus
Genetic Insert: Snrp40
Vector Backbone Description: Backbone Size:8800; Vector Backbone:pBOB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31427773
Proper citation: RRID:Addgene_134249 Copy
Species: Mus musculus
Genetic Insert: Snrp40
Vector Backbone Description: Backbone Size:8800; Vector Backbone:pBOB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31427773
Proper citation: RRID:Addgene_134250 Copy
Species: Mus musculus
Genetic Insert: Snrp40
Vector Backbone Description: Backbone Marker:BioSettia; Backbone Size:8800; Vector Backbone:pLV-EF1a -MCS-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31427773
Proper citation: RRID:Addgene_134253 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pYTKMV1; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31433622
Comments: The plasmid contains a small insertion 3' of the insert that results in the C-terminal NLS being unfused to the ORF.
Proper citation: RRID:Addgene_134420 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pYTKMV2; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31433622
Comments: The plasmid contains a small insertion 3' of the insert that results in the C-terminal NLS being unfused to the ORF.
Proper citation: RRID:Addgene_134424 Copy
Species: Synthetic
Genetic Insert: A1
Vector Backbone Description: Vector Backbone:pFA6a-natMX4; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32401365
Proper citation: RRID:Addgene_134428 Copy
Species: Synthetic
Genetic Insert: A1-FLAG
Vector Backbone Description: Vector Backbone:pFA6a-hphMX4; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32401365
Proper citation: RRID:Addgene_134429 Copy
Species: Synthetic
Genetic Insert: S6
Vector Backbone Description: Vector Backbone:pFA6a-kanMX6; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32401365
Proper citation: RRID:Addgene_134434 Copy
Species: Synthetic
Genetic Insert: S6
Vector Backbone Description: Vector Backbone:pFA6a-natMX4; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32401365
Proper citation: RRID:Addgene_134436 Copy
Species: Synthetic
Genetic Insert: S6-FLAG
Vector Backbone Description: Vector Backbone:pFA6a-hphMX4; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32401365
Proper citation: RRID:Addgene_134437 Copy
Species: Synthetic
Genetic Insert: S6-FLAG
Vector Backbone Description: Vector Backbone:pFA6a-kanMX6; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32401365
Proper citation: RRID:Addgene_134438 Copy
Species: Mus musculus
Genetic Insert: Cdk6
Vector Backbone Description: Backbone Marker:Sen Wu, Capecchi laboratory; Vector Backbone:pWS-TK6; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32183906
Comments: Homology arms subcloned from a clone of RPCI-24 C57BL/6J mouse genomic DNA library.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
Mice available from The Jackson Laboratory (https://www.jax.org/strain/036458).
Proper citation: RRID:Addgene_134321 Copy
Species: Synthetic
Genetic Insert: SRS
Vector Backbone Description: Vector Backbone:pFA6a-natMX4; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32401365
Proper citation: RRID:Addgene_134444 Copy
Species: Synthetic
Genetic Insert: SRS-FLAG
Vector Backbone Description: Vector Backbone:pFA6a-hphMX4; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32401365
Proper citation: RRID:Addgene_134445 Copy
Species: Synthetic
Genetic Insert: SRS-FLAG
Vector Backbone Description: Vector Backbone:pFA6a-natMX4; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32401365
Proper citation: RRID:Addgene_134448 Copy
Vector Backbone Description: Backbone Marker:Feng Zhang; Vector Backbone:pSpCas9(BB)-2A-GFP (PX458); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32150173
Comments: The sgRNAs can get introduced by golden gate cloning like described in Andersson-Rolf A et al.: Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids. Dev Biol. 2016. doi: 10.1016/j.ydbio.2016.10.016. For more information see protocol.
Proper citation: RRID:Addgene_134449 Copy
Species: Synthetic
Genetic Insert: human codon optimized AcrIIA17_Sga
Vector Backbone Description: Vector Backbone:JDS246 (Addgene plasmid #43861); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32218510
Proper citation: RRID:Addgene_134334 Copy
Species: Synthetic
Genetic Insert: human codon optimized AcrIIA19_Spse
Vector Backbone Description: Vector Backbone:JDS246 (Addgene plasmid #43861); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32218510
Proper citation: RRID:Addgene_134339 Copy
Species: Homo sapiens
Genetic Insert: dnCDK5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_1344 Copy
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