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Species: Homo sapiens
Genetic Insert: CENP-L and His-CENP-N
Vector Backbone Description: Vector Backbone:pACEBAC1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:26698661
Proper citation: RRID:Addgene_134205 Copy
Species: Mus musculus
Genetic Insert: Gk5
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pMBP-parallel1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28607088
Proper citation: RRID:Addgene_134290 Copy
Species: Mus musculus
Genetic Insert: Gk5
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pMBP-parallel1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28607088
Proper citation: RRID:Addgene_134293 Copy
Species: Homo sapiens
Genetic Insert: CENP-H/K/M untagged + his-I
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:26698661
Proper citation: RRID:Addgene_134210 Copy
Species: Other
Genetic Insert: mOrange
Vector Backbone Description: Vector Backbone:pFUW-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23770078
Proper citation: RRID:Addgene_134298 Copy
Species: Homo sapiens
Genetic Insert: CENP-N-his-CENP-L
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:26698661
Proper citation: RRID:Addgene_134211 Copy
Species: Homo sapiens
Genetic Insert: CENP HIKM
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:26698661
Proper citation: RRID:Addgene_134219 Copy
Species: Homo sapiens
Genetic Insert: P25
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_1343 Copy
Species: Homo sapiens
Genetic Insert: GST-CENP-C 1-509
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:26698661
Proper citation: RRID:Addgene_134221 Copy
Species: Homo sapiens
Genetic Insert: GST-CENP-C 510-end
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:26698661
Proper citation: RRID:Addgene_134222 Copy
Species: Homo sapiens
Genetic Insert: GST-CENP-C 235-509
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:26698661
Proper citation: RRID:Addgene_134224 Copy
Species: Homo sapiens
Genetic Insert: mCherry-HJURP-LacI 1-304
Vector Backbone Description: Vector Backbone:pkg196; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_134227 Copy
Species: Homo sapiens
Genetic Insert: DK360 tdTomato (long SKAP)
Vector Backbone Description: Backbone Marker:Erik Dent (Addgene plasmid # 50708); Vector Backbone:EB3-tdTomato; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_134229 Copy
Species: Patiria miniata (Starfish)
Genetic Insert: Miniata CENP-A n-terminus (nt 1-204, aa 1-68)
Vector Backbone Description: Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31422918
Proper citation: RRID:Addgene_134233 Copy
Species: Patiria miniata (Starfish)
Genetic Insert: GST-CENP-C
Vector Backbone Description: Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31422918
Proper citation: RRID:Addgene_134235 Copy
Species: Homo sapiens
Genetic Insert: Human HJURP sgRNA
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini (Addgene plasmid # 71409) ; Vector Backbone:pLENTI-sgRNA; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31422918
Proper citation: RRID:Addgene_134238 Copy
Species: Homo sapiens
Genetic Insert: CENP A 3' CRISPR construct
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 42230); Vector Backbone:px330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31422918
Proper citation: RRID:Addgene_134240 Copy
Species: Homo sapiens
Genetic Insert: p85 alpha (delta BH)
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16880400
Comments: see author's map for more information.
Proper citation: RRID:Addgene_13431 Copy
Species: Homo sapiens
Genetic Insert: gRNA targeting PLK1 3'
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 42230); Vector Backbone:px330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31422918
Proper citation: RRID:Addgene_134243 Copy
Species: Homo sapiens
Genetic Insert: Human H3.1A + eGFP/HALO retroviral tagging
Vector Backbone Description: Vector Backbone:pBABE-Blast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31422918
Proper citation: RRID:Addgene_134244 Copy
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