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Species: Viral
Genetic Insert: SV40L
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Terminator
Proper citation: RRID:Addgene_165798 Copy
Species: Synthetic
Genetic Insert: EBFP2
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: CDS
Proper citation: RRID:Addgene_165831 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: CDS-TL1
Proper citation: RRID:Addgene_165832 Copy
Species: Synthetic
Genetic Insert: Promoterless-2A
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Linker - Promoter Mimic
Proper citation: RRID:Addgene_165790 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: CDS-TL5
Proper citation: RRID:Addgene_165835 Copy
Species: E. Coli
Genetic Insert: nptII
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: CDS
Proper citation: RRID:Addgene_165836 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: CDS-TL1
Proper citation: RRID:Addgene_165834 Copy
Species: E. Coli
Genetic Insert: hph
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: CDS
Proper citation: RRID:Addgene_165839 Copy
Species: E. Coli
Genetic Insert: pac
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: CDS
Proper citation: RRID:Addgene_165837 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:6204; Vector Backbone:P[acman]; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Omega1
Proper citation: RRID:Addgene_165860 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:6204; Vector Backbone:P[acman]; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Omega2
Proper citation: RRID:Addgene_165861 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:1941; Vector Backbone:pR6Kg; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Alpha1
Proper citation: RRID:Addgene_165862 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10400; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This construct can be used to express a double HA-tagged protein of interest, either as a C-terminal or N-terminal addition.
Proper citation: RRID:Addgene_16586 Copy
Species: Homo sapiens
Genetic Insert: PTTG1
Vector Backbone Description: Backbone Size:3939; Vector Backbone:pFredB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: 5 kb 3' targeting element, should be linearized with SalI prior to transfection.
Proper citation: RRID:Addgene_16585 Copy
Species: P1 bacteriophage
Genetic Insert: cre recombinase
Vector Backbone Description: Backbone Size:7700; Vector Backbone:na; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments: Cre expression vector.
Proper citation: RRID:Addgene_16583 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3939; Vector Backbone:pFredB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Targeting vector derived from pBluescript-SK. Contains 3' neomycin gene and tk polyA signal. pFredA and pFredB make up the “two-vector” targeting system developed in an effort to reduce the background rate of Geneticin-resistant colonies arising from nonhomologous integration events.
Proper citation: RRID:Addgene_16582 Copy
Species: Homo sapiens
Genetic Insert: c-MYC binding sites
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Different combinations of oligonucleotide pairs (A+B, A+D, C+B, C+D) were annealed and converted to double-stranded fragments through one PCR cycle. These promoter fragments were subcloned into pBV-luc. Further polymerase-derived mutants were identified while sequencing the reporter constructs.
Proper citation: RRID:Addgene_16566 Copy
Species: Homo sapiens
Genetic Insert: c-MYC binding sites
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: To generate reporter constructs, the following oligonucleotides were used:
5'-CCGGTACCGG GTTGTGGCAG CCAGTCACCT GCCCGCCGCG TAGCCACACC TCTGCTCCTC AGAGCAATGT CAAGCGGTCA CCTGTGATAG CAACAGATCA CCTGGCTGCC ATCGCCCCTC-3' [Oligo C , for mutant MBS1-3];
5'-ATGAATTCCG GACGTTCTGG GCAGGTGACC GCCACCCATG CGCTGAGGGG CGGACAGGAG GTGCTTCGAC TGGGAGGAGG GCGAAGAGTG TAAGGGGGCG GAGGGGCGAT GGCAGCCAGG-3' [Oligo D, for mutant MBS4].
Different combinations of oligonucleotide pairs (A+B, A+D, C+B, C+D) were annealed and converted to double-stranded fragments through one PCR cycle. These promoter fragments were subcloned into pBV-luc. Further polymerase-derived mutants were identified while sequencing the reporter constructs.
Proper citation: RRID:Addgene_16565 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4699; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD130.04, Ligation number L4506.
Proper citation: RRID:Addgene_1658 Copy
Species: Homo sapiens
Genetic Insert: c-MYC binding sites
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: To generate reporter constructs, the following oligonucleotides were used:
5'-CCGGTACCGG GTTGTGGCAG CCAGTCACGT GCCCGCCGCG TAGCCACACC TCTGCTCCTC AGAGCAATGT CAAGCGGTCA CGTGTGATAG CAACAGATCA CGTGGCTGCC ATCGCCCCTC-3' [Oligo A, for wild-type c-MYC binding sites (MBS)1-3]; 5'-ATGAATTCCG GACGTTCTGG GCACGTGACC GCCACCCATG CGCTGAGGGG CGGACAGGAG GTGCTTCGAC TGGGAGGAGG GCGAAGAGTG TAAGGGGGCG GAGGGGCGAT GGCAGCC-3' [Oligo B, for wild-type MBS4].
Different combinations of oligonucleotide pairs (A+B, A+D, C+B, C+D) were annealed and converted to double-stranded fragments through one PCR cycle. These promoter fragments were subcloned into pBV-luc. Further polymerase-derived mutants were identified while sequencing the reporter constructs.
Proper citation: RRID:Addgene_16564 Copy
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