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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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CMV-Citron1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_134303 | Citron1 | Ampicillin | PMID:32875085 | Backbone Size:5300; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-19 02:09:18 | 3 | |||
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CMV-mito-Citron1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_134305 | Citron1 | Synthetic | Ampicillin | PMID:32875085 | Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-19 02:09:18 | 3 | ||
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pFudio-Cpg15-IRES2-TdTomatoW Resource Report Resource Website |
RRID:Addgene_134309 | cpg15 | Mus musculus | Ampicillin | PMID:31390571 | Backbone Size:9534; Vector Backbone:pFUW; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin | 2026-09-19 02:09:18 | 0 | ||
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pST1374 Resource Report Resource Website 1+ mentions |
RRID:Addgene_13426 | Ampicillin | PMID:17406455 | Human zinc finger nuclease cloning vector. Zinc fingers are cloned in between unique XbaI and BamHI sites in front of the FokI nuclease domain. | Backbone Size:5725; Vector Backbone:pST1374; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-19 02:09:17 | 9 | |||
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CAG pShuttle Resource Report Resource Website 1+ mentions |
RRID:Addgene_13427 | Kanamycin | pShuttle is used to make adenovirus in the AdEasy system. The CAG promoter was inserted for stronger expression and a MCS compatible with pBS. See author's map for more information. | Backbone Size:0; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin | 2026-09-19 02:09:18 | 2 | ||||
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pBOB-N-Flag-GK_v1 Resource Report Resource Website |
RRID:Addgene_134270 | Gk | Mus musculus | Ampicillin | PMID:28607088 | Backbone Size:8800; Vector Backbone:pBOB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | mouse Gk isoform 1; 524 amino acids | 2026-09-19 02:09:18 | 0 | |
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KJBAC1 strain Resource Report Resource Website |
RRID:Addgene_13424 | KJBAC1 Strain | Tetracycline | PMID:17406455 | E. coli bacterial strain that constitutively expresses high levels of lac repressor. Genotype: F- lacIq delta hisB463 delta(gpt-proAB-arg-lac) XIII zaj::Tn10. Use 12.5 ug/ml tetracycline for growth. | Backbone Size:0; Vector Backbone:na; Vector Types:; Bacterial Resistance:Tetracycline | 2026-09-19 02:09:17 | 0 | ||
|
pBAC-lacZ Resource Report Resource Website 1+ mentions |
RRID:Addgene_13422 | Chloramphenicol | PMID:17406455 | The complete assembled sequence for this plasmid may not be accurate. Addgene suggests sequence verifying the construct before starting your experiments. Bacterial cell-based two-hybrid (B2H) reporter vector. Use 12.5 ug/ml chloramphenicol for growth. The pBAC-lacZ plasmid is a mini-F' that can be replicated in standard E. coli strains, but because it is maintained as a single copy episome, it gives low DNA yields. pBAC-lacZ also contains a second, higher copy number origin of replication (oriV) that is only active in the presence of a trans-acting factor encoded by the trfA gene. Transformax EPI300 cells express trfA from an inducible promoter that we have found is inducible with arabinose. When the trfA gene is induced in Transformax EPI300 cells, the copy number of pBAC-lacZ plasmid is increased in the cells and reasonable yields of plasmid can be obtained using a standard miniprep procedure. Further notes on propagating this plasmid: grow an overnight culture (without arabinose) and then the next morning subculture 1:10 into medium that has a final concentration of arabinose of 1 mM. You then grow this culture for 5 to 6 hours at 37 C and then harvest the DNA. | Backbone Size:11151; Vector Backbone:pBAC-lacZ; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol | 2026-09-19 02:09:17 | 5 | |||
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pBOB-N-Flag-GK (281-473) Resource Report Resource Website |
RRID:Addgene_134273 | Gk | Mus musculus | Ampicillin | PMID:28607088 | Backbone Size:8800; Vector Backbone:pBOB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | mouse Gk amino acids 281-473 | 2026-09-19 02:09:18 | 0 | |
|
pAC-Kan-alphaGal4 Resource Report Resource Website |
RRID:Addgene_13421 | RNAP alpha-Gal4 | Kanamycin | PMID:17406455 | Expresses E. coli RNAP alpha subunit aa 1-248 fused to yeast Gal4 aa 58-97. This plasmid requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter. | Backbone Size:3600; Vector Backbone:pACLU-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-09-19 02:09:17 | 0 | ||
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pBOB-N-3HA-GK_v2 Resource Report Resource Website |
RRID:Addgene_134277 | Gk | Mus musculus | Ampicillin | PMID:28607088 | Backbone Size:8800; Vector Backbone:pBOB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 553 amino acids, isoform 2 | 2026-09-19 02:09:18 | 0 | |
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pSiMPlk_N + pSiMPlk_C Resource Report Resource Website |
RRID:Addgene_134310 | kanR_gp41-1 N-intein + kanR_gp41-1 C-intein | Synthetic | Kanamycin | PMID:31672972 | pSiMPlk_N should be cotransformed with pSiMPlk_C (or vice versa) in E. coli to get a reconstituted functional APT thereby conferring resistance against kanamycin. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252). Please see the Sequences link for the individual plasmid sequences. The sample from Addgene will have both the plasmids pSiMPlk_N + pSiMPlk_C in it for the interested antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones. | Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-09-19 02:09:18 | 0 | |
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pcDNA6-GK5_v1 Resource Report Resource Website |
RRID:Addgene_134279 | Gk5 | Mus musculus | Ampicillin | PMID:28607088 | Backbone Size:5000; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | mouse Gk5 isoform 1; 516 amino acids | 2026-09-19 02:09:18 | 0 | |
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pSiMPlc_N + pSiMPlc_C Resource Report Resource Website |
RRID:Addgene_134312 | cmR_gp41-1 N-intein + cmR_gp41-1 C-intein | Synthetic | Chloramphenicol | PMID:31672972 | pSiMPlc_N should be cotransformed with pSiMPlc_C (or vice versa) in E. Coli to get a reconstituted functional CAT thereby conferring resistance against chloramphenicol. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252). Please see the Sequences link for the individual plasmid sequences. The sample from Addgene will have both the plasmids pSiMPlc_N + pSiMPlc_C in it for the interested antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones. | Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-09-19 02:09:18 | 0 | |
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U6 pShuttle Resource Report Resource Website 1+ mentions |
RRID:Addgene_13428 | Kanamycin | pShuttle is used to make adenovirus in the AdEasy system. The human U6 promoter allows for siRNA expression. | Backbone Size:0; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin | 2026-09-19 02:09:18 | 2 | ||||
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pcDNA6-GK5_v2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_134280 | Gk5 | Mus musculus | Ampicillin | PMID:28607088 | Backbone Size:5000; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | mouse Gk5 isoform 2; 534 amino acids | 2026-09-19 02:09:18 | 1 | |
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pcDNA6-C-Flag-GK5_v1 Resource Report Resource Website |
RRID:Addgene_134281 | Gk5 | Mus musculus | Ampicillin | PMID:28607088 | Backbone Size:5000; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | mouse Gk5 isoform 1; 516 amino acids | 2026-09-19 02:09:18 | 0 | |
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pcDNA6-N-Flag-SREBP1 Resource Report Resource Website |
RRID:Addgene_134283 | SREBP1 | Mus musculus | Ampicillin | PMID:28607088 | Backbone Size:5000; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | mouse SREBP1 isoform a precursor | 2026-09-19 02:09:18 | 0 | |
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pcDNA6-N-Flag-SREBP2 Resource Report Resource Website |
RRID:Addgene_134284 | SREBP2 | Mus musculus | Ampicillin | PMID:28607088 | Backbone Size:5000; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | mouse SREBP2 precursor | 2026-09-19 02:09:18 | 0 | |
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pLV-puro-N-3HA-SREBP2-C-Flag Resource Report Resource Website |
RRID:Addgene_134287 | SREBP2 | Mus musculus | Ampicillin | PMID:28607088 | Backbone Marker:BioSettia; Backbone Size:8900; Vector Backbone:pLV-EF1a -MCS-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | mouse SREBP2 precursor | 2026-09-19 02:09:18 | 0 |
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