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http://www.addgene.org/134303

Genetic Insert: Citron1
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32875085

Proper citation: RRID:Addgene_134303 Copy   

•   Source: Addgene

http://www.addgene.org/134305

Species: Synthetic
Genetic Insert: Citron1
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32875085

Proper citation: RRID:Addgene_134305 Copy   

•   Source: Addgene

http://www.addgene.org/134309

Species: Mus musculus
Genetic Insert: cpg15
Vector Backbone Description: Backbone Size:9534; Vector Backbone:pFUW; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31390571

Proper citation: RRID:Addgene_134309 Copy   

•   Source: Addgene

http://www.addgene.org/13426

Vector Backbone Description: Backbone Size:5725; Vector Backbone:pST1374; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17406455
Comments: Human zinc finger nuclease cloning vector. Zinc fingers are cloned in between unique XbaI and BamHI sites in front of the FokI nuclease domain.

Proper citation: RRID:Addgene_13426 Copy   

•   Source: Addgene

http://www.addgene.org/13427

Vector Backbone Description: Backbone Size:0; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Comments: pShuttle is used to make adenovirus in the AdEasy system. The CAG promoter was inserted for stronger expression and a MCS compatible with pBS. See author's map for more information.

Proper citation: RRID:Addgene_13427 Copy   

•   Source: Addgene

http://www.addgene.org/134270

Species: Mus musculus
Genetic Insert: Gk
Vector Backbone Description: Backbone Size:8800; Vector Backbone:pBOB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28607088

Proper citation: RRID:Addgene_134270 Copy   

•   Source: Addgene

http://www.addgene.org/13424

Genetic Insert: KJBAC1 Strain
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:17406455
Comments: E. coli bacterial strain that constitutively expresses high levels of lac repressor. Genotype: F- lacIq delta hisB463 delta(gpt-proAB-arg-lac) XIII zaj::Tn10. Use 12.5 ug/ml tetracycline for growth.

Proper citation: RRID:Addgene_13424 Copy   

•   Source: Addgene

http://www.addgene.org/13422

Vector Backbone Description: Backbone Size:11151; Vector Backbone:pBAC-lacZ; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17406455
Comments: The complete assembled sequence for this plasmid may not be accurate. Addgene suggests sequence verifying the construct before starting your experiments. Bacterial cell-based two-hybrid (B2H) reporter vector. Use 12.5 ug/ml chloramphenicol for growth. The pBAC-lacZ plasmid is a mini-F' that can be replicated in standard E. coli strains, but because it is maintained as a single copy episome, it gives low DNA yields. pBAC-lacZ also contains a second, higher copy number origin of replication (oriV) that is only active in the presence of a trans-acting factor encoded by the trfA gene. Transformax EPI300 cells express trfA from an inducible promoter that we have found is inducible with arabinose. When the trfA gene is induced in Transformax EPI300 cells, the copy number of pBAC-lacZ plasmid is increased in the cells and reasonable yields of plasmid can be obtained using a standard miniprep procedure. Further notes on propagating this plasmid: grow an overnight culture (without arabinose) and then the next morning subculture 1:10 into medium that has a final concentration of arabinose of 1 mM. You then grow this culture for 5 to 6 hours at 37 C and then harvest the DNA.

Proper citation: RRID:Addgene_13422 Copy   

•   Source: Addgene

http://www.addgene.org/134273

Species: Mus musculus
Genetic Insert: Gk
Vector Backbone Description: Backbone Size:8800; Vector Backbone:pBOB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28607088

Proper citation: RRID:Addgene_134273 Copy   

•   Source: Addgene

http://www.addgene.org/13421

Genetic Insert: RNAP alpha-Gal4
Vector Backbone Description: Backbone Size:3600; Vector Backbone:pACLU-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17406455
Comments: Expresses E. coli RNAP alpha subunit aa 1-248 fused to yeast Gal4 aa 58-97. This plasmid requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter.

Proper citation: RRID:Addgene_13421 Copy   

•   Source: Addgene

http://www.addgene.org/134277

Species: Mus musculus
Genetic Insert: Gk
Vector Backbone Description: Backbone Size:8800; Vector Backbone:pBOB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28607088

Proper citation: RRID:Addgene_134277 Copy   

•   Source: Addgene

http://www.addgene.org/134310

Species: Synthetic
Genetic Insert: kanR_gp41-1 N-intein + kanR_gp41-1 C-intein
Vector Backbone Description: Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31672972
Comments: pSiMPlk_N should be cotransformed with pSiMPlk_C (or vice versa) in E. coli to get a reconstituted functional APT thereby conferring resistance against kanamycin. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252). Please see the Sequences link for the individual plasmid sequences. The sample from Addgene will have both the plasmids pSiMPlk_N + pSiMPlk_C in it for the interested antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.

Proper citation: RRID:Addgene_134310 Copy   

•   Source: Addgene

http://www.addgene.org/134279

Species: Mus musculus
Genetic Insert: Gk5
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28607088

Proper citation: RRID:Addgene_134279 Copy   

•   Source: Addgene

http://www.addgene.org/134312

Species: Synthetic
Genetic Insert: cmR_gp41-1 N-intein + cmR_gp41-1 C-intein
Vector Backbone Description: Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31672972
Comments: pSiMPlc_N should be cotransformed with pSiMPlc_C (or vice versa) in E. Coli to get a reconstituted functional CAT thereby conferring resistance against chloramphenicol. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252). Please see the Sequences link for the individual plasmid sequences. The sample from Addgene will have both the plasmids pSiMPlc_N + pSiMPlc_C in it for the interested antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.

Proper citation: RRID:Addgene_134312 Copy   

•   Source: Addgene

http://www.addgene.org/13428

Vector Backbone Description: Backbone Size:0; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Comments: pShuttle is used to make adenovirus in the AdEasy system. The human U6 promoter allows for siRNA expression.

Proper citation: RRID:Addgene_13428 Copy   

•   Source: Addgene

http://www.addgene.org/134280

Species: Mus musculus
Genetic Insert: Gk5
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28607088

Proper citation: RRID:Addgene_134280 Copy   

•   Source: Addgene

http://www.addgene.org/134281

Species: Mus musculus
Genetic Insert: Gk5
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28607088

Proper citation: RRID:Addgene_134281 Copy   

•   Source: Addgene

http://www.addgene.org/134283

Species: Mus musculus
Genetic Insert: SREBP1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28607088

Proper citation: RRID:Addgene_134283 Copy   

•   Source: Addgene

http://www.addgene.org/134284

Species: Mus musculus
Genetic Insert: SREBP2
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28607088

Proper citation: RRID:Addgene_134284 Copy   

•   Source: Addgene

http://www.addgene.org/134287

Species: Mus musculus
Genetic Insert: SREBP2
Vector Backbone Description: Backbone Marker:BioSettia; Backbone Size:8900; Vector Backbone:pLV-EF1a -MCS-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28607088

Proper citation: RRID:Addgene_134287 Copy   

•   Source: Addgene