Plasmids   Select Another Resource Report Type

http://www.addgene.org/165591

Species: Mus musculus
Genetic Insert: P300
Vector Backbone Description: Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_165591 Copy   

•   Source: Addgene

http://www.addgene.org/165515

Species: Other
Genetic Insert: M.EcoRI
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Methylase that recognizes the double-stranded sequence GAATTC, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the EcoRI endonuclease. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165515 Copy   

•   Source: Addgene

http://www.addgene.org/165518

Species: Other
Genetic Insert: M.NotI
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Site-specific DNA methyltransferase; protects DNA from cleavage by NotI. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165518 Copy   

•   Source: Addgene

http://www.addgene.org/165546

Species: Other
Genetic Insert: Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165546 Copy   

•   Source: Addgene

http://www.addgene.org/165549

Species: Other
Genetic Insert: Extreme Thermostable Single-Stranded DNA Binding Protein
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificity of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165549 Copy   

•   Source: Addgene

http://www.addgene.org/165548

Species: Other
Genetic Insert: EcoRV
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Type II restriction enzyme that recognizes the double-stranded sequence GATATC and cleaves after T-1. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165548 Copy   

•   Source: Addgene

http://www.addgene.org/165531

Species: Other
Genetic Insert: UDG
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Used in PreCR mix from NEB, Uracil DNA glycosylase; Monofunctional DNA glycosylase that catalyzes the hydrolysis of the N-glycosidic bond from deoxyuridine to release uracil; Active on ss and dsDNA; Heat inactivation not possible. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165531 Copy   

•   Source: Addgene

http://www.addgene.org/16559

Species: Homo sapiens
Genetic Insert: TCF-4 binding sites
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: TCF-4 mutant binding sequence - CCTTTGGC

Proper citation: RRID:Addgene_16559 Copy   

•   Source: Addgene

http://www.addgene.org/165534

Species: Other
Genetic Insert: T7 DNA Polymerase (unmodified)
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: DNA Polymerase that catalyzes the replication of T7 phage DNA during infection. The protein dimer has two catalytic activities: DNA polymerase activity and strong 3'-5' exonuclease. The high fidelity and rapid extension rate of the enzyme make it particularly useful in copying long stretches of DNA template. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165534 Copy   

•   Source: Addgene

http://www.addgene.org/16557

Species: Homo sapiens
Genetic Insert: MADMYC
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: MADMYC: the amino terminal transactivation domain of c-Myc (aa 1-263) was removed and replaced with the amino terminal transcriptional repression domain (aa 1-38, the Sin3-interaction region) of Mad.

Proper citation: RRID:Addgene_16557 Copy   

•   Source: Addgene

http://www.addgene.org/16554

Species: Homo sapiens
Genetic Insert: CDX2
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:7462; Vector Backbone:pHRCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
References:
Comments: RKO (colorectal cancer cell line) mutation: 4 base-pair deletion (AGGCAGG to AGG) within the sequence corresponding to helix 3 of the CDX2 homeodomain.

Proper citation: RRID:Addgene_16554 Copy   

•   Source: Addgene

http://www.addgene.org/16553

Species: Homo sapiens
Genetic Insert: CDX2
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:7462; Vector Backbone:pHRCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_16553 Copy   

•   Source: Addgene

http://www.addgene.org/16552

Species: Homo sapiens
Genetic Insert: 14-3-3 sigma
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:7462; Vector Backbone:pHRCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_16552 Copy   

•   Source: Addgene

http://www.addgene.org/165538

Species: Other
Genetic Insert: M.SfiI
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Site-specific DNA methyltransferase; protects DNA from cleavage by SfiI. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165538 Copy   

•   Source: Addgene

http://www.addgene.org/165843

Species: Viral
Genetic Insert: _C31 attB
Vector Backbone Description: Backbone Size:2171; Vector Backbone:pColE1_Omega1; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: attB docking site

Proper citation: RRID:Addgene_165843 Copy   

•   Source: Addgene

http://www.addgene.org/165840

Species: E. Coli
Genetic Insert: ble
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: CDS

Proper citation: RRID:Addgene_165840 Copy   

•   Source: Addgene

http://www.addgene.org/165841

Species: Viral
Genetic Insert: SR39TK
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: CDS

Proper citation: RRID:Addgene_165841 Copy   

•   Source: Addgene

http://www.addgene.org/165794

Species: Synthetic
Genetic Insert: CP6 Promoter
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Bacterial Promoter

Proper citation: RRID:Addgene_165794 Copy   

•   Source: Addgene

http://www.addgene.org/165795

Species: Synthetic
Genetic Insert: CP6 promoter
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Bacterial Promoter

Proper citation: RRID:Addgene_165795 Copy   

•   Source: Addgene

http://www.addgene.org/165792

Species: Drosophila melanogaster
Genetic Insert: R70B04
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Enhancer

Proper citation: RRID:Addgene_165792 Copy   

•   Source: Addgene