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Species: Homo sapiens
Genetic Insert: Kif18A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_23002 Copy
Species: Homo sapiens
Genetic Insert: Kif18A-mut
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_23003 Copy
Species:
Genetic Insert: Subunit 9 of the F0-ATPase
Vector Backbone Description: Backbone Size:5000; Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_23214 Copy
Species: Homo sapiens
Genetic Insert: USP15
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pcDNA-4C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_23216 Copy
Species: Mus musculus
Genetic Insert: Mfn1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pcDNA3.1(-)/Myc-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_23212 Copy
Species: Homo sapiens
Genetic Insert: Rnd3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_23229 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4604; Vector Backbone:pUHD-P3; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: This vector is designed for dual expression of a shRNA and the respective rescue cDNA to allow a one-step validation of shRNAs. The shRNA is expressed under the control of a mouse U6 RNA promoter, while the rescue cDNA is expressed under the control of a doxycycline-inducible promoter. The effects of gene knockdown by the shRNA are effectively under conditional control as cDNA expression can be regulated by the inducible promoter.
The shRNA sequence must be cloned into this plasmid using BbsI and XbaI sites BEFORE cloning the cDNA, otherwise the NcoI, XhoI, EcoRI and BamHI sites will not be unique for subcloning of the cDNA. Note that the cloning of the shRNA sequence into the plasmid will replace the EGFP in this plasmid.
Proper citation: RRID:Addgene_23104 Copy
Species: Cricetulus griseus (Chinese hamster)
Genetic Insert: Mitotic Centromere Associated Kinesin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4709; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_23107 Copy
Species: Homo sapiens
Genetic Insert: Rnd2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_23228 Copy
Species: Mus musculus
Genetic Insert: Bim EL (3SA)
Vector Backbone Description: Backbone Size:5057; Vector Backbone:pGSTag; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_23100 Copy
Species: artifical
Genetic Insert: SnAvi
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5050; Vector Backbone:pTriEx5; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_23221 Copy
Species: Mus musculus
Genetic Insert: Bim EL (TA)
Vector Backbone Description: Backbone Size:5057; Vector Backbone:pGSTag; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_23101 Copy
Species: Mus musculus
Genetic Insert: Bim EL (TD)
Vector Backbone Description: Backbone Size:5057; Vector Backbone:pGSTag; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_23102 Copy
Species: Caenorhabditis elegans
Genetic Insert: SnAvi
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9674; Vector Backbone:EGFP-N1; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments: this plasmid encodes the new described SnAvi tag and additionally the unc-119 rescue sequence from C. elegans (bp4-5705)
Proper citation: RRID:Addgene_23223 Copy
Species: Homo sapiens
Genetic Insert: RhoA
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_23224 Copy
Species: Top10 E.coli, Discosoma sp.
Genetic Insert: BirA-mCherry
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3992; Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_23220 Copy
Species: Helicobacter pylori
Genetic Insert: TNF-α-inducing protein
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_23159 Copy
Species: Helicobacter pylori
Genetic Insert: TNF-α-inducing protein
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_23157 Copy
Species: Helicobacter pylori
Genetic Insert: TNF-α-inducing protein
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_23158 Copy
Species: Mus musculus
Genetic Insert: mouse gammaF crystallin promoter:mCherry
Vector Backbone Description: Backbone Size:0; Vector Backbone:p817 (pBSII derivate); Vector Types:multicloning site flanked by I-SceI sites; Bacterial Resistance:Ampicillin
References:
Comments: A=>G and G=>A mismatches in gene do not have any functional significance.
Proper citation: RRID:Addgene_23154 Copy
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