Searching the RRID Resource Information Network
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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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px330 BRM gRNA Resource Report Resource Website |
RRID:Addgene_165586 | BRM | Mus musculus | Ampicillin | PMID:33334824 | Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | NA | 2026-08-01 01:07:34 | 0 | |
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px330 MAU2 gRNA Resource Report Resource Website |
RRID:Addgene_165589 | MAU2 | Mus musculus | Ampicillin | PMID:33334824 | Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | NA | 2026-08-01 01:07:34 | 0 | |
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px330 CBP gRNA Resource Report Resource Website |
RRID:Addgene_165587 | CBP | Mus musculus | Ampicillin | PMID:33334824 | Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | NA | 2026-08-01 01:07:28 | 0 | |
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px330 CTCF gRNA Resource Report Resource Website |
RRID:Addgene_165588 | CTCF | Mus musculus | Ampicillin | PMID:33334824 | Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | NA | 2026-08-01 01:07:28 | 0 | |
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pOpen-RNAse H Resource Report Resource Website |
RRID:Addgene_165575 | RNAse H | Other | Ampicillin | Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Participates in DNA replication; helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus. Along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis, and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA pre-primer. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:28 | 0 | ||
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pOpen-GBDpol (exo) Resource Report Resource Website |
RRID:Addgene_165572 | Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase | Other | Ampicillin | Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:28 | 0 | ||
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pNA0304 Resource Report Resource Website |
RRID:Addgene_165612 | SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer) | Ampicillin | PMID:33441553 | This plasmid was constructed by Dr. Agmon in the Boeke lab and first described in: Agmon, N. et al. Phylogenetic debugging of a complete human biosynthetic pathway transplanted into yeast. Nucleic Acids Res. 48, 486–499 (2020) | Backbone Marker:Church Lab (Addgene #43803); Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin | NotI site replaced the CAN1 spacer in parent vector (to facilitate insertion of new spacers via Gibson assembly) | 2026-08-01 01:07:28 | 0 | |
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pOpen-T3gene1 Resource Report Resource Website |
RRID:Addgene_165576 | T3 RNA Polymerase | Other | Ampicillin | DNA-dependent RNA polymerase derived from T3 bacteriophage that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:28 | 0 | ||
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pOpen-XbaIM Resource Report Resource Website |
RRID:Addgene_165577 | M.XbaI | Other | Ampicillin | Site-specific DNA methyltransferase; protects DNA from cleavage by XbaI. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:34 | 0 | ||
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pOpen-T4 PNK Resource Report Resource Website |
RRID:Addgene_165520 | T4 PNK | Other | Ampicillin | The most commonly used polynucleotide kinase. Used for: 5' phosphorylation of DNA/RNA for subsequent ligation; end labeling DNA or RNA for probes and DNA sequencing; removal of 3' phosphoryl groups. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:27 | 0 | ||
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pOpen-Tlipol (exo-) Resource Report Resource Website |
RRID:Addgene_165523 | Tli DNA polymerase (exo-) | Other | Ampicillin | DNA polymerase; lacks 3' to 5' exonuclease activity. Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the 'homing site' in gene that lack the intein. Comparable to VentR (exo-) DNA Polymerase at NEB. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:27 | 0 | ||
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pOpen-lambda red operon Resource Report Resource Website |
RRID:Addgene_165521 | lambda red operon | Other | Ampicillin | Operon containing Exo, Bet, and Gam. To use the lambda red recombineering system to modify your target DNA (including E. coli genome), electroporate a linear donor DNA substrate (either dsDNA or ssDNA) into E. coli expressing the lambda red enzymes. These enzymes then catalyze the homologous recombination of the substrate with the target DNA sequence (mutagenesis occurs in vivo). ~50 nucleotides of homology are required to the target site for recombination. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:27 | 0 | ||
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pIVEX2.3 TEM-5 Resource Report Resource Website |
RRID:Addgene_16561 | TEM5 | Homo sapiens | Ampicillin | PMID:11559528 | Backbone Marker:Boehringer; Backbone Size:3553; Vector Backbone:pIVEX2.3-MCS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Extracellular region | 2026-08-01 01:07:34 | 0 | |
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pGEX TEM-1 Resource Report Resource Website |
RRID:Addgene_16560 | TEM1 | Homo sapiens | Ampicillin | PMID:11559528 | Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Extracellular region | 2026-08-01 01:07:28 | 0 | |
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pOpen-Eco31IB Resource Report Resource Website |
RRID:Addgene_165527 | Eco31IB | Other | Ampicillin | Site-specific DNA methyltransferase 2; protects DNA from cleavage by Eco31I. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:27 | 0 | ||
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pOpen-SfiIR (cDNA) Resource Report Resource Website |
RRID:Addgene_165528 | SfiI (used to create cDNA libraries) | Other | Ampicillin | Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:33 | 0 | ||
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pOpen-Pfulig Resource Report Resource Website |
RRID:Addgene_165525 | Pfu DNA Ligase | Other | Ampicillin | DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. Catalyzes template-dependent ligation at temperatures of about 30-80 degrees C, and substantially retains its catalytic activity when subjected to temperatures of about 85 degrees C to about 100 degrees C. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:33 | 0 | ||
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pOpen-THEAQpolA Resource Report Resource Website |
RRID:Addgene_165512 | Taq DNA Polymerase | Other | Ampicillin | DNA polymerase used in (routine) PCR method because of its high thermostability, standard testing, screening and output testing. Has a relatively high error rate because it lacks 3'-5' exonuclease activity and proofreading functionality. 5'-3' flap endonuclease degrades displaced strand. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/ and https://freegenes.github.io/genes/BBF10K_003257.html. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:27 | 0 | ||
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pOpen-K12polLF Resource Report Resource Website |
RRID:Addgene_165511 | DNA Polymerase I, Large (Klenow) Fragment | Other | Ampicillin | DNA polymerase fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity for removal of precoding nucleotides and proofreading, but loses 5'-3' exonuclease activity. Usable for synthesis of double-stranded DNA from single-stranded templates, filling in of receded 3' ends of DNA fragments to make 5' overhang blunt, digesting away protruding 3' overhangs, preparation of radioactive DNA probes. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:27 | 0 | ||
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px330 RAD21 gRNA Resource Report Resource Website |
RRID:Addgene_165592 | RAD21 | Mus musculus | Ampicillin | PMID:33334824 | Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | NA | 2026-08-01 01:07:28 | 0 |
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