Plasmids   Select Another Resource Report Type

http://www.addgene.org/165586

Species: Mus musculus
Genetic Insert: BRM
Vector Backbone Description: Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_165586 Copy   

•   Source: Addgene

http://www.addgene.org/165589

Species: Mus musculus
Genetic Insert: MAU2
Vector Backbone Description: Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_165589 Copy   

•   Source: Addgene

http://www.addgene.org/165587

Species: Mus musculus
Genetic Insert: CBP
Vector Backbone Description: Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_165587 Copy   

•   Source: Addgene

http://www.addgene.org/165588

Species: Mus musculus
Genetic Insert: CTCF
Vector Backbone Description: Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_165588 Copy   

•   Source: Addgene

http://www.addgene.org/165575

Species: Other
Genetic Insert: RNAse H
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Participates in DNA replication; helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus. Along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis, and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA pre-primer. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165575 Copy   

•   Source: Addgene

http://www.addgene.org/165572

Species: Other
Genetic Insert: Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165572 Copy   

•   Source: Addgene

http://www.addgene.org/165612

Species:
Genetic Insert: SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
Vector Backbone Description: Backbone Marker:Church Lab (Addgene #43803); Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid was constructed by Dr. Agmon in the Boeke lab and first described in: Agmon, N. et al. Phylogenetic debugging of a complete human biosynthetic pathway transplanted into yeast. Nucleic Acids Res. 48, 486–499 (2020)

Proper citation: RRID:Addgene_165612 Copy   

•   Source: Addgene

http://www.addgene.org/165576

Species: Other
Genetic Insert: T3 RNA Polymerase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: DNA-dependent RNA polymerase derived from T3 bacteriophage that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165576 Copy   

•   Source: Addgene

http://www.addgene.org/165577

Species: Other
Genetic Insert: M.XbaI
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Site-specific DNA methyltransferase; protects DNA from cleavage by XbaI. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165577 Copy   

•   Source: Addgene

http://www.addgene.org/165520

Species: Other
Genetic Insert: T4 PNK
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: The most commonly used polynucleotide kinase. Used for: 5' phosphorylation of DNA/RNA for subsequent ligation; end labeling DNA or RNA for probes and DNA sequencing; removal of 3' phosphoryl groups. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165520 Copy   

•   Source: Addgene

http://www.addgene.org/165523

Species: Other
Genetic Insert: Tli DNA polymerase (exo-)
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: DNA polymerase; lacks 3' to 5' exonuclease activity. Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the 'homing site' in gene that lack the intein. Comparable to VentR (exo-) DNA Polymerase at NEB. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165523 Copy   

•   Source: Addgene

http://www.addgene.org/165521

Species: Other
Genetic Insert: lambda red operon
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Operon containing Exo, Bet, and Gam. To use the lambda red recombineering system to modify your target DNA (including E. coli genome), electroporate a linear donor DNA substrate (either dsDNA or ssDNA) into E. coli expressing the lambda red enzymes. These enzymes then catalyze the homologous recombination of the substrate with the target DNA sequence (mutagenesis occurs in vivo). ~50 nucleotides of homology are required to the target site for recombination. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165521 Copy   

•   Source: Addgene

http://www.addgene.org/16561

Species: Homo sapiens
Genetic Insert: TEM5
Vector Backbone Description: Backbone Marker:Boehringer; Backbone Size:3553; Vector Backbone:pIVEX2.3-MCS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_16561 Copy   

•   Source: Addgene

http://www.addgene.org/16560

Species: Homo sapiens
Genetic Insert: TEM1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_16560 Copy   

•   Source: Addgene

http://www.addgene.org/165527

Species: Other
Genetic Insert: Eco31IB
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Site-specific DNA methyltransferase 2; protects DNA from cleavage by Eco31I. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165527 Copy   

•   Source: Addgene

http://www.addgene.org/165528

Species: Other
Genetic Insert: SfiI (used to create cDNA libraries)
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165528 Copy   

•   Source: Addgene

http://www.addgene.org/165525

Species: Other
Genetic Insert: Pfu DNA Ligase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. Catalyzes template-dependent ligation at temperatures of about 30-80 degrees C, and substantially retains its catalytic activity when subjected to temperatures of about 85 degrees C to about 100 degrees C. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165525 Copy   

•   Source: Addgene

http://www.addgene.org/165512

Species: Other
Genetic Insert: Taq DNA Polymerase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: DNA polymerase used in (routine) PCR method because of its high thermostability, standard testing, screening and output testing. Has a relatively high error rate because it lacks 3'-5' exonuclease activity and proofreading functionality. 5'-3' flap endonuclease degrades displaced strand. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/ and https://freegenes.github.io/genes/BBF10K_003257.html.

Proper citation: RRID:Addgene_165512 Copy   

•   Source: Addgene

http://www.addgene.org/165511

Species: Other
Genetic Insert: DNA Polymerase I, Large (Klenow) Fragment
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: DNA polymerase fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity for removal of precoding nucleotides and proofreading, but loses 5'-3' exonuclease activity. Usable for synthesis of double-stranded DNA from single-stranded templates, filling in of receded 3' ends of DNA fragments to make 5' overhang blunt, digesting away protruding 3' overhangs, preparation of radioactive DNA probes. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165511 Copy   

•   Source: Addgene

http://www.addgene.org/165592

Species: Mus musculus
Genetic Insert: RAD21
Vector Backbone Description: Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_165592 Copy   

•   Source: Addgene