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http://www.addgene.org/165491

Species: Synthetic
Genetic Insert: 2xLyn-ERex-Venus-bPAC(F198Y)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4697; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_165491 Copy   

•   Source: Addgene

http://www.addgene.org/165492

Species: Synthetic
Genetic Insert: 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4697; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid is identical to PACmn but with a point mutation in Venus to reduce fluorescence by about 90% to facilitate combining with fluorescent indicators etc. It was not used in the original publication.

Proper citation: RRID:Addgene_165492 Copy   

•   Source: Addgene

http://www.addgene.org/165417

Species: Synthetic
Genetic Insert: ABE8e-NRCH
Vector Backbone Description: Backbone Marker:Atum; Backbone Size:2253; Vector Backbone:pD881-SR; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_165417 Copy   

•   Source: Addgene

http://www.addgene.org/165415

Species: Gallus gallus
Genetic Insert: GgPCFT_NB
Vector Backbone Description: Backbone Size:6994; Vector Backbone:pBXNPHM3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_165415 Copy   

•   Source: Addgene

http://www.addgene.org/165562

Species: Other
Genetic Insert: Pwo DNA Polymerase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: DNA polymerase; additionally exhibits 3' to 5' exonuclease activity. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165562 Copy   

•   Source: Addgene

http://www.addgene.org/165567

Species: Other
Genetic Insert: XbaI
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Type II restriction enzyme that recognizes the double-stranded sequence TCTAGA and cleaves after T-1. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165567 Copy   

•   Source: Addgene

http://www.addgene.org/165568

Species: Other
Genetic Insert: NcoI
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Type II restriction enzyme that recognizes the double-stranded sequence CCATGG and cleaves after C-1. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165568 Copy   

•   Source: Addgene

http://www.addgene.org/165601

Species: Homo sapiens
Genetic Insert: ribosomal protein S6 kinase A5
Vector Backbone Description: Vector Backbone:LentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_165601 Copy   

•   Source: Addgene

http://www.addgene.org/165565

Species: Other
Genetic Insert: RNAse A
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA. Effectively cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide which forms a 2',3'-cyclic phosphate which is then hydrolyzed to the corresponding 3'-nucleoside phosphate. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165565 Copy   

•   Source: Addgene

http://www.addgene.org/165566

Species: Other
Genetic Insert: Thermus thermophilus (Tth) RT
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: DNA polymerase that efficiently reverse-transcribes RNA in the presence of MnCl2. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165566 Copy   

•   Source: Addgene

http://www.addgene.org/165560

Species: Other
Genetic Insert: T4 gene 32
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: A single-stranded DNA binding protein required for bacteriophage T4 replication and repair; Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure, to increase yield and processivity of reverse transcriptase during RT-PCR, and to increase yield and specificity of PCR products from soil samples. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165560 Copy   

•   Source: Addgene

http://www.addgene.org/165604

Species:
Genetic Insert: hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
Vector Backbone Description: Vector Backbone:pGHUC_R25; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_165604 Copy   

•   Source: Addgene

http://www.addgene.org/165605

Species:
Genetic Insert: hEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
Vector Backbone Description: Backbone Marker:Marcus Noyes Lab (Addgene #165604); Vector Backbone:pGG184; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_165605 Copy   

•   Source: Addgene

http://www.addgene.org/165569

Species: Other
Genetic Insert: Tth DNA Ligase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: High fidelity version Taq DNA Ligase. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165569 Copy   

•   Source: Addgene

http://www.addgene.org/165606

Species:
Genetic Insert: ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
Vector Backbone Description: Backbone Marker:Marcus Noyes Lab; Vector Backbone:pB1H2_UV2-ω-dCas9::K2_UV5-sgRNA; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_165606 Copy   

•   Source: Addgene

http://www.addgene.org/165550

Species: Other
Genetic Insert: T4 PGD (T4 Endonuclease V)
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: T4 pyrimidine DNA glycosylase; bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities. The N-glycosylase activity releases cis-syn cyclobutane pyrimidine dimers, including T^T, T^C and C^C, generating an AP site. The AP lyase activity cleaves an AP site via beta-elimination, creating a 1 nucleotide DNA gap with 3'-alpha, beta-unsaturated aldehyde and 5'-phosphate termini. Also known as T4 Endonuclease V. This enzyme in combination with other enzymes from E. coli (UDG, fpg, Endonuclease IV, Endonuclease VIII), Bst-DNA pol and Taq/Tth-DNA Ligase forms the proprietary NEB Enzyme mix preCR. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165550 Copy   

•   Source: Addgene

http://www.addgene.org/165551

Species: Other
Genetic Insert: Endonuclease IV E. coli
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Used in the preCR mix from NEB; DNA AP endonuclease; Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini; Also has 3'-diesterase activity which can remove 3' phosphate, 3'-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165551 Copy   

•   Source: Addgene

http://www.addgene.org/165556

Species: Other
Genetic Insert: Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165556 Copy   

•   Source: Addgene

http://www.addgene.org/165558

Species: Other
Genetic Insert: M.EcoRV
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Site-specific DNA methyltransferase; protects DNA from cleavage by EcoRV. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165558 Copy   

•   Source: Addgene

http://www.addgene.org/165585

Species: Mus musculus
Genetic Insert: BRG1
Vector Backbone Description: Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_165585 Copy   

•   Source: Addgene