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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pAAV Syn 2xLyn-ERex-Venus-bPAC(F198Y) Resource Report Resource Website |
RRID:Addgene_165491 | 2xLyn-ERex-Venus-bPAC(F198Y) | Synthetic | Ampicillin | PMID:34663304 | Backbone Marker:Stratagene; Backbone Size:4697; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:33 | 0 | ||
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pAAV-Syn-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y) Resource Report Resource Website |
RRID:Addgene_165492 | 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y) | Synthetic | Ampicillin | PMID:34663304 | This plasmid is identical to PACmn but with a point mutation in Venus to reduce fluorescence by about 90% to facilitate combining with fluorescent indicators etc. It was not used in the original publication. | Backbone Marker:Stratagene; Backbone Size:4697; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:27 | 0 | |
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pRha-ABE8e-NRCH Resource Report Resource Website |
RRID:Addgene_165417 | ABE8e-NRCH | Synthetic | Kanamycin | Backbone Marker:Atum; Backbone Size:2253; Vector Backbone:pD881-SR; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-01 01:07:26 | 0 | |||
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pBXPHM3-PCFT_NB Resource Report Resource Website |
RRID:Addgene_165415 | GgPCFT_NB | Gallus gallus | Ampicillin | PMID:34040256 | Backbone Size:6994; Vector Backbone:pBXNPHM3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:33 | 0 | ||
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pOpen-Pwopol Resource Report Resource Website |
RRID:Addgene_165562 | Pwo DNA Polymerase | Other | Ampicillin | DNA polymerase; additionally exhibits 3' to 5' exonuclease activity. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:28 | 0 | ||
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pOpen-XbaIR Resource Report Resource Website |
RRID:Addgene_165567 | XbaI | Other | Ampicillin | Type II restriction enzyme that recognizes the double-stranded sequence TCTAGA and cleaves after T-1. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:28 | 0 | ||
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pOpen-NcoIR Resource Report Resource Website |
RRID:Addgene_165568 | NcoI | Other | Ampicillin | Type II restriction enzyme that recognizes the double-stranded sequence CCATGG and cleaves after C-1. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:28 | 0 | ||
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dCas9-MSK1 Resource Report Resource Website |
RRID:Addgene_165601 | ribosomal protein S6 kinase A5 | Homo sapiens | Ampicillin | PMID:33563994 | Vector Backbone:LentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:28 | 0 | ||
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pOpen-RNAse A Resource Report Resource Website |
RRID:Addgene_165565 | RNAse A | Other | Ampicillin | Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA. Effectively cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide which forms a 2',3'-cyclic phosphate which is then hydrolyzed to the corresponding 3'-nucleoside phosphate. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:28 | 0 | ||
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pOpen-TthRT Resource Report Resource Website |
RRID:Addgene_165566 | Thermus thermophilus (Tth) RT | Other | Ampicillin | DNA polymerase that efficiently reverse-transcribes RNA in the presence of MnCl2. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:34 | 0 | ||
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pOpen-T4 g32 Resource Report Resource Website |
RRID:Addgene_165560 | T4 gene 32 | Other | Ampicillin | A single-stranded DNA binding protein required for bacteriophage T4 replication and repair; Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure, to increase yield and processivity of reverse transcriptase during RT-PCR, and to increase yield and specificity of PCR products from soil samples. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:34 | 0 | ||
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pGG184 Resource Report Resource Website |
RRID:Addgene_165604 | hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter | Kanamycin | PMID:33441553 | Vector Backbone:pGHUC_R25; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin | 'CAGCG' PAM replacing the 'CGGCG' PAM in pGHUC_R25 | 2026-08-01 01:07:28 | 0 | ||
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pGG198 Resource Report Resource Website |
RRID:Addgene_165605 | hEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter | Kanamycin | PMID:33441553 | Backbone Marker:Marcus Noyes Lab (Addgene #165604); Vector Backbone:pGG184; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin | Secondary protospacer ('CGGCG' PAM) inserted downstream of the HIS3/GFP promoter | 2026-08-01 01:07:28 | 0 | ||
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pOpen-Tthlig Resource Report Resource Website |
RRID:Addgene_165569 | Tth DNA Ligase | Other | Ampicillin | High fidelity version Taq DNA Ligase. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:34 | 0 | ||
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pGG369 Resource Report Resource Website 1+ mentions |
RRID:Addgene_165606 | ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA | Ampicillin | PMID:33441553 | Backbone Marker:Marcus Noyes Lab; Vector Backbone:pB1H2_UV2-ω-dCas9::K2_UV5-sgRNA; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin | Recoding of BsaI site in the AmpR cassette, two BsaI sites in place of the sgRNA's spacer, and KanR disruption in PID replaced with intact PID from Wt SpCas9 | 2026-08-01 01:07:34 | 1 | ||
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pOpen-T4 PGD Resource Report Resource Website |
RRID:Addgene_165550 | T4 PGD (T4 Endonuclease V) | Other | Ampicillin | T4 pyrimidine DNA glycosylase; bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities. The N-glycosylase activity releases cis-syn cyclobutane pyrimidine dimers, including T^T, T^C and C^C, generating an AP site. The AP lyase activity cleaves an AP site via beta-elimination, creating a 1 nucleotide DNA gap with 3'-alpha, beta-unsaturated aldehyde and 5'-phosphate termini. Also known as T4 Endonuclease V. This enzyme in combination with other enzymes from E. coli (UDG, fpg, Endonuclease IV, Endonuclease VIII), Bst-DNA pol and Taq/Tth-DNA Ligase forms the proprietary NEB Enzyme mix preCR. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:27 | 0 | ||
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pOpen-Endonuclease IV E. coli Resource Report Resource Website |
RRID:Addgene_165551 | Endonuclease IV E. coli | Other | Ampicillin | Used in the preCR mix from NEB; DNA AP endonuclease; Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini; Also has 3'-diesterase activity which can remove 3' phosphate, 3'-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:33 | 0 | ||
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pOpen-MMLV_RT (lack H) Resource Report Resource Website |
RRID:Addgene_165556 | Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) | Other | Ampicillin | Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:28 | 0 | ||
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pOpen-EcoRVM Resource Report Resource Website |
RRID:Addgene_165558 | M.EcoRV | Other | Ampicillin | Site-specific DNA methyltransferase; protects DNA from cleavage by EcoRV. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-01 01:07:34 | 0 | ||
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px330BRG1 gRNA Resource Report Resource Website |
RRID:Addgene_165585 | BRG1 | Mus musculus | Ampicillin | PMID:33334824 | Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | NA | 2026-08-01 01:07:28 | 0 |
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