Plasmids   Select Another Resource Report Type

http://www.addgene.org/45896

Species: Homo sapiens
Genetic Insert: Nck-1 M3 mutant
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11959995
Comments: Wild type pEGFP-Nck1 (Addgene plasmid 45903) is described in JBC 26:26633-44 2006 (PMID 16835242). Mutations are described in the article linked to this plasmid (PMID 11959995) and then subcloned into the pEGFP-C1 vector using PCR amplification with primers containing XhoI and EcoRI sites.

Proper citation: RRID:Addgene_45896 Copy   

•   Source: Addgene

http://www.addgene.org/45778

Genetic Insert: Tetracycline repressor
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_45778 Copy   

•   Source: Addgene

http://www.addgene.org/45811

Species: Homo sapiens
Genetic Insert: hα1Ga
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pDsRed-Express-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: The Cav3.1 insert retains its STOP codon, so there is no expression of the Ds-Red. The 5'UTR is gone and contains a new SalI site. The 3' end of insert ends in the endogenous Kpn1 site. Identification of Cav3.1 is described in Nature. 1998 Feb 26;391(6670):896-900.

Proper citation: RRID:Addgene_45811 Copy   

•   Source: Addgene

http://www.addgene.org/45899

Species: Mus musculus
Genetic Insert: eIF2beta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3500; Vector Backbone:pZeoSV2; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:11959995
Comments: Two fragments were PCR amplified from GST-eIF2beta (Addgene plasmid 45900) and ligated together with vector to incorporate deletion. Xba1 site is used to ligate the 2 parts of eIF2beta.

Proper citation: RRID:Addgene_45899 Copy   

•   Source: Addgene

http://www.addgene.org/45770

Species: Homo sapiens
Genetic Insert: hE-cadherin/Δp120
Vector Backbone Description: Vector Backbone:pLKpac1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11381089
Comments: The three-alanine substitution present in this plasmid prevent the hE-cadherin gene product from interacting with p120 catenin. Alternate plasmid name: hE-cadherin-762AAA-pcDNA3

Proper citation: RRID:Addgene_45770 Copy   

•   Source: Addgene

http://www.addgene.org/45925

Species: Saccharomyces cerevisiae
Genetic Insert: yeast Vps23
Vector Backbone Description: Backbone Size:6103; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21505419

Proper citation: RRID:Addgene_45925 Copy   

•   Source: Addgene

http://www.addgene.org/45920

Species: Mus musculus
Genetic Insert: vesicle-associated membrane protein 7
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6600; Vector Backbone:pMRXIP GFP-Ci2; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23217709

Proper citation: RRID:Addgene_45920 Copy   

•   Source: Addgene

http://www.addgene.org/45919

Species: Homo sapiens
Genetic Insert: vesicle-associated membrane protein 8
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6600; Vector Backbone:pMRXIP GFP-Ci2; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23217709

Proper citation: RRID:Addgene_45919 Copy   

•   Source: Addgene

http://www.addgene.org/46041

Species: Mus musculus
Genetic Insert: nonmuscle myosin heavy chain II-C, isoform C1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16790446

Proper citation: RRID:Addgene_46041 Copy   

•   Source: Addgene

http://www.addgene.org/46044

Species: Mus musculus
Genetic Insert: nonmuscle myosin heavy chain II-C, isoform C1C2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19240025

Proper citation: RRID:Addgene_46044 Copy   

•   Source: Addgene

http://www.addgene.org/46042

Species: K. lactis
Genetic Insert: Atg1 EAT domain
Vector Backbone Description: Backbone Size:5550; Vector Backbone:pHIS-parallel.2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23219485

Proper citation: RRID:Addgene_46042 Copy   

•   Source: Addgene

http://www.addgene.org/45988

Vector Backbone Description: Backbone Size:6925; Vector Backbone:pOSIP; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:24050148
Comments: Reference: St-Pierre F, Cui L et al., "One-step cloning and chromosomal integration of DNA", ACS Synthetic Biology, http://pubs.acs.org/doi/abs/10.1021/sb400021j

Proper citation: RRID:Addgene_45988 Copy   

•   Source: Addgene

http://www.addgene.org/46035

Species: Homo sapiens
Genetic Insert: WRN K577M
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pBI; Vector Types:Mammalian Expression, TetOn, bidirectional promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15084309
Comments: Bidirectional vector expresses WRN and GFP at similar expression levels. In the associated article, cells were transiently transfected with this plasmid together with a tTA transactivator plasmid (Gossen, 1992). This is a mammalian expression vector for helicase-dead human WRN protein. It was constructed from pBI, a Clontech plasmid with a bi-directional promoter+ TRE backbone, modified by insertion of a polylinker containing BspD1 and Xhol sites followed by insertion of a myc-WRN K577M ORF- created by making K577M (A>T) mutation in pMM290 sequence. The S2G mutation was a result of the cloning strategy to add the myc tag. The WRN protein containing S2G was biochemically verified and is wild-type in terms of activities, as described in the associated publication.

Proper citation: RRID:Addgene_46035 Copy   

•   Source: Addgene

http://www.addgene.org/45982

Vector Backbone Description: Backbone Size:7145; Vector Backbone:pOSIP; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24050148
Comments: Reference: St-Pierre F, Cui L et al., "One-step cloning and chromosomal integration of DNA", ACS Synthetic Biology, http://pubs.acs.org/doi/abs/10.1021/sb400021j

Proper citation: RRID:Addgene_45982 Copy   

•   Source: Addgene

http://www.addgene.org/46030

Species: Homo sapiens
Genetic Insert: GATA4
Vector Backbone Description: Vector Backbone:tetO-Gateway; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23591016
Comments: Please note that Addgene's sequencing identified R43Q, G50S and N197S mutations in the GATA4 insert in this plasmid when compared to GenBank reference sequence NP_002043.2. These difference were present in the original GATA4 sample and the plasmid functions as described in the associated publication.

Proper citation: RRID:Addgene_46030 Copy   

•   Source: Addgene

http://www.addgene.org/46031

Species: Homo sapiens
Genetic Insert: MEF2C
Vector Backbone Description: Vector Backbone:tetO-Gateway; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23591016

Proper citation: RRID:Addgene_46031 Copy   

•   Source: Addgene

http://www.addgene.org/45978

Genetic Insert: flp
Vector Backbone Description: Vector Backbone:pKD46; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24050148
Comments: Can be used to remove the integration module (including the antibiotic resistance cassette) from pOSIP integrants. Reference: St-Pierre F, Cui L et al., "One-step cloning and chromosomal integration of DNA", ACS Synthetic Biology, http://pubs.acs.org/doi/abs/10.1021/sb400021j

Proper citation: RRID:Addgene_45978 Copy   

•   Source: Addgene

http://www.addgene.org/45977

Species: Rattus norvegicus
Genetic Insert: Parkin W403A-C431S
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23661642

Proper citation: RRID:Addgene_45977 Copy   

•   Source: Addgene

http://www.addgene.org/46026

Vector Backbone Description: Vector Backbone:FUdeltaGW-rtTA; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23591016
Comments: Please note that Addgene's sequencing results identified nucleotide mismatches at bp# 3473, 3937, 4087 & 4822 when compared to the full plasmid sequence. According to the depositing laboratory, these differences are not a concern for the function of the plasmid.

Proper citation: RRID:Addgene_46026 Copy   

•   Source: Addgene

http://www.addgene.org/45972

Species: Rattus norvegicus
Genetic Insert: Parkin 219-465
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23661642

Proper citation: RRID:Addgene_45972 Copy   

•   Source: Addgene