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Species: Rattus norvegicus
Genetic Insert: mEGFP(A206K)-paCaMKII(K42M)
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Only tested in HeLa cells, but not in neurons.
Proper citation: RRID:Addgene_165440 Copy
Species: Other
Genetic Insert: EGFP-NLS
Vector Backbone Description: Backbone Marker:Roger Tsien; Vector Backbone:AAV-flex-ReaChR-citrine (Addgene 50955); Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165441 Copy
Species: Rattus norvegicus
Genetic Insert: tdTomato-P2A-paCaMKII
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165431 Copy
Species: Rattus norvegicus
Genetic Insert: tdTomato-P2A-paCaMKII(T286A)
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165432 Copy
Species: Rattus norvegicus
Genetic Insert: FHS-paCaMKII(SD)
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165430 Copy
Species: Homo sapiens
Genetic Insert: ShadowY-RBD-P2A-Clover(T153M/F223R)-RhoA
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165435 Copy
Species: Rattus norvegicus
Genetic Insert: tdTomato-P2A-paCaMKII(K42M)
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165433 Copy
Species: Homo sapiens
Genetic Insert: ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165434 Copy
Species: Rattus norvegicus
Genetic Insert: mEGFP(A206K)-paCaMKII
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Only tested in HeLa cells, but not in neurons.
Proper citation: RRID:Addgene_165438 Copy
Species: Homo sapiens
Genetic Insert: Heterochromatin protein 1 binding protein 3
Vector Backbone Description: Backbone Marker:Stratagene /Agilent; Backbone Size:5200; Vector Backbone:pCMV-3Tag-8 modified MCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Backbone contains new multiple cloning site and c-terminal HA epitope instead of FLAGs. Construction: XhoI-ACC-[HP1BP3 CDS 1650 bp, no stop]-Noti-a-Clai-HA epitope tag-ctt-STOP. T3 forward primer is too close for sequencing of initiation sequence.
Proper citation: RRID:Addgene_165468 Copy
Species: Other
Genetic Insert: 9 degrees N-7 DNA Polymerase chain terminating
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: 9 Degrees North DNA polymerase variant with an enhanced ability to incorporate modified substrates such as dideoxynucleotides, ribonucleotides and acyclonucleotides. Comparable to Therminator DNA Pol at NEB.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165502 Copy
Species: Homo sapiens
Genetic Insert: TANGO1S
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES_neo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165461 Copy
Species: Other
Genetic Insert: DNA Ligase IV
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Ligase used in efficiently joining single-strand breaks in a double-strand polydeoxynucleotide in an ATP-dependent reaction. Also involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165503 Copy
Species: Other
Genetic Insert: HindIII
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Type II restriction enzyme that recognizes the double-stranded sequence AAGCTT and cleaves after A-1.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165508 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC19; Vector Types:Bacterial vector for DNA production; Bacterial Resistance:Ampicillin
References:
Comments: Please cite the following article in any publications where this construct is utilized:
doi: 10.1093/nar/gkab070
Z. Adhireksan, D. Sharma, P.L. Lee, Q. Bao, S. Padavattan, W.K. Shum, G.E. Davey & C.A. Davey. (2021). Engineering Nucleosomes for Generating Diverse Chromatin Assemblies. Nucleic Acids Res.
Proper citation: RRID:Addgene_165452 Copy
Species: Other
Genetic Insert: CRE
Vector Backbone Description: Backbone Size:10785; Vector Backbone:AAVS1-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165457 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC19; Vector Types:Bacterial vector for DNA production; Bacterial Resistance:Ampicillin
References:
Comments: Please cite the following article in any publications where this construct is utilized:
doi: 10.1093/nar/gkab070
Z. Adhireksan, D. Sharma, P.L. Lee, Q. Bao, S. Padavattan, W.K. Shum, G.E. Davey & C.A. Davey. (2021). Engineering Nucleosomes for Generating Diverse Chromatin Assemblies. Nucleic Acids Res.
Proper citation: RRID:Addgene_165455 Copy
Species: Homo sapiens
Genetic Insert: ZEB2
Vector Backbone Description: Backbone Size:11520; Vector Backbone:AAVS1-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165456 Copy
Species:
Genetic Insert: Mammalian codon-optimized Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFlag
Vector Backbone Description: Backbone Marker:Joung Lab (Addgene Plasmid #65771); Vector Backbone:MSP469; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165486 Copy
Species: Synthetic
Genetic Insert: Venus-bPAC(F198Y)-myc
Vector Backbone Description: Backbone Size:3100; Vector Backbone:pGEM HE; Vector Types:Bacterial Expression, cDNA expression, Xenopus Oocyte; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165487 Copy
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