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Species: Synthetic
Genetic Insert: flag-GFP 1-10 tetra Cys
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pRK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27295555
Proper citation: RRID:Addgene_78589 Copy
Species: Synthetic
Genetic Insert: J106-30hrpR-32hrpS-t-hrpL-30gfp-t
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030903
Proper citation: RRID:Addgene_78651 Copy
Species: Synthetic
Genetic Insert: J105-30gfp-t
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030903
Proper citation: RRID:Addgene_78644 Copy
Species: Synthetic
Genetic Insert: J106-30gfp-t
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030903
Proper citation: RRID:Addgene_78645 Copy
Species: Synthetic
Genetic Insert: J116-30gfp-t
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030903
Proper citation: RRID:Addgene_78642 Copy
Species: Synthetic
Genetic Insert: J114-30gfp-t
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030903
Proper citation: RRID:Addgene_78641 Copy
Species: Synthetic
Genetic Insert: J114-30hrpR-32hrpS-t-hrpL-30gfp-t
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030903
Proper citation: RRID:Addgene_78647 Copy
Species: Synthetic
Genetic Insert: Insulated J23100 Promoter
Vector Backbone Description: Backbone Size:2023; Vector Backbone:DVA; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28422998
Proper citation: RRID:Addgene_78666 Copy
Species: Synthetic
Genetic Insert: BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
Vector Backbone Description: Backbone Marker:Registry of Standardized Biological Parts; Backbone Size:2047; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27111289
Proper citation: RRID:Addgene_78691 Copy
Species: Synthetic
Genetic Insert: BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
Vector Backbone Description: Backbone Marker:Registry of Standardized Biological Parts; Backbone Size:2047; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27111289
Proper citation: RRID:Addgene_78693 Copy
Species: Synthetic
Genetic Insert: BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
Vector Backbone Description: Backbone Marker:Registry of Standardized Biological Parts; Backbone Size:2047; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27111289
Proper citation: RRID:Addgene_78692 Copy
Species: Synthetic
Genetic Insert: BBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
Vector Backbone Description: Backbone Marker:Registry of Standardized Biological Parts; Backbone Size:2047; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27111289
Proper citation: RRID:Addgene_78699 Copy
Species: Synthetic
Genetic Insert: cytGAP
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24501126
Comments: GAP is a fusion of two Aequorea victoria proteins, GFP and apo-aequorin, with a linker in between. The excitation spectrum of GAP maintained the two peaks of wtGFP, at 403 and 470 nm, and it was sensitive to Ca2+.
Proper citation: RRID:Addgene_78734 Copy
Species: Synthetic
Genetic Insert: BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
Vector Backbone Description: Backbone Marker:Registry of Standardized Biological Parts; Backbone Size:2047; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27111289
Proper citation: RRID:Addgene_78695 Copy
Species: Synthetic
Genetic Insert: BBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
Vector Backbone Description: Backbone Marker:Registry of Standardized Biological Parts; Backbone Size:2047; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27111289
Proper citation: RRID:Addgene_78697 Copy
Species: Synthetic
Genetic Insert: BBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
Vector Backbone Description: Backbone Marker:Registry of Standardized Biological Parts; Backbone Size:2047; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27111289
Proper citation: RRID:Addgene_78696 Copy
Species: Synthetic
Genetic Insert: tgoGAP1
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24501126
Proper citation: RRID:Addgene_78737 Copy
Species: Synthetic
Genetic Insert: Insulated pTet Promoter
Vector Backbone Description: Backbone Size:2023; Vector Backbone:DVA; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28422998
Proper citation: RRID:Addgene_78680 Copy
Species: Synthetic
Genetic Insert: LbCpf1 crRNA backbone, without spacer sequence
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 43860); Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27347757
Comments: Use BsmBI sites to insert crRNA spacer sequence into the vector. This plasmid will express gRNA in mammalian cells for Cpf1 from Lachnospiraceae bacterium ND2006. Recommended to use with SQT1665 (Addgene plasmid# 78744), which expresses Lachnospiraceae bacterium ND2006 Cpf1.
Please visit https://www.biorxiv.org/content/early/2016/06/09/057802 for bioRxiv preprint.
Proper citation: RRID:Addgene_78742 Copy
Species: Synthetic
Genetic Insert: Insulated pBAD Promoter
Vector Backbone Description: Backbone Size:2023; Vector Backbone:DVA; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28422998
Proper citation: RRID:Addgene_78677 Copy
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