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Species: Synthetic
Genetic Insert: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
Vector Backbone Description: Vector Backbone:pMOD_C0000; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91086 Copy
Species: Synthetic
Genetic Insert: Esp3I ccdb cassette for single gRNA spacer cloning
Vector Backbone Description: Vector Backbone:pMOD_C0000; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91085 Copy
Species: Synthetic
Genetic Insert: TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning
Vector Backbone Description: Vector Backbone:pMOD_B2000; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91080 Copy
Species: Synthetic
Genetic Insert: Esp3I ccdb cassette for single gRNA spacer cloning
Vector Backbone Description: Vector Backbone:pMOD_B2000; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Comments: Please note: This plasmid may be prone to multimerization. This is not expected to impact the end function of the plasmid. Please refer to the Addgene Blog post on multimers for more information: https://blog.addgene.org/plasmids-101-dimers-and-multimers
Proper citation: RRID:Addgene_91075 Copy
Species: Synthetic
Genetic Insert: Esp3I ccdb cassette for single gRNA spacer cloning
Vector Backbone Description: Vector Backbone:pMOD_B2000; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91076 Copy
Species: Synthetic
Genetic Insert: SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers
Vector Backbone Description: Vector Backbone:pMOD_B2000; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91071 Copy
Species: Synthetic
Genetic Insert: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
Vector Backbone Description: Vector Backbone:pMOD_B2000; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91070 Copy
Species: Synthetic
Genetic Insert: 35S:AtCas9 + AtU6:gRNA
Vector Backbone Description: Vector Backbone:pTRANS_100; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91207 Copy
Species: Synthetic
Genetic Insert: nAtCas9_D10A+gRNActrl+donor
Vector Backbone Description: Vector Backbone:pTRANS_201; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91187 Copy
Species: Synthetic
Genetic Insert: TALENs targeting tomato ANT1
Vector Backbone Description: Vector Backbone:pTC110; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91178 Copy
Species: Synthetic
Genetic Insert: Codon optimized dCas9-Mxi1
Vector Backbone Description: Backbone Size:2623; Vector Backbone:pUC19; Vector Types:Yeast Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28832943
Proper citation: RRID:Addgene_91248 Copy
Species: Synthetic
Genetic Insert: Codon optimized dCas9-Mxi1
Vector Backbone Description: Backbone Size:2623; Vector Backbone:pUC19; Vector Types:Yeast Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28832943
Proper citation: RRID:Addgene_91249 Copy
Species: Synthetic
Genetic Insert: SV40-CMV-SaCas9-3xNLS
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26721686
Proper citation: RRID:Addgene_78601 Copy
Species: Synthetic
Genetic Insert: U6-SpAi9L-U6SpDMDL
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR blunt II TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26721686
Proper citation: RRID:Addgene_78608 Copy
Species: Synthetic
Genetic Insert: U6-SpAi9R-U6-SpDMDR
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR blunt II TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26721686
Proper citation: RRID:Addgene_78609 Copy
Species: Synthetic
Genetic Insert: gRNAs for SaCas9 targeting Ai9 locus
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26721686
Proper citation: RRID:Addgene_78604 Copy
Species: Synthetic
Genetic Insert: CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26721686
Comments: gRNA targeting sequences:
CAGTAATGTGTCATACCTTC;
ATATAATAGAAATTATTCAT
Proper citation: RRID:Addgene_78606 Copy
Species: Synthetic
Genetic Insert: J101-rbs32-arsR-t-ParsR
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030903
Proper citation: RRID:Addgene_78635 Copy
Species: Synthetic
Genetic Insert: 30hrpR-32hrpS-t-hrpL-30gfp-t
Vector Backbone Description: Backbone Marker:Wang Lab; Vector Backbone:pBW100ParsR; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030903
Proper citation: RRID:Addgene_78637 Copy
Species: Synthetic
Genetic Insert: araC-PBAD
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030903
Proper citation: RRID:Addgene_78639 Copy
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