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Species: Homo sapiens
Genetic Insert: p53 binding site
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4900; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This reporter contains 3 copies of the wt p53-binding site from the 14-3-3 sigma promoter. The following oligonucleotide pairs were used: 5'-CCTGTAGCAT TAGCCCAGAC ATGTCCCTAC TCCGTAC-3' and 5'-GGAGTAGGGA CATGTCTGGG CTAATGCTAC AGGGTAC-3' for BDS-2.
Proper citation: RRID:Addgene_16515 Copy
Species: Homo sapiens
Genetic Insert: TCF4
Vector Backbone Description: Backbone Size:9200; Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: The TCF4 in this construct has a Q27R mutation. It is not known to affect the function.
According to the depositing scientist, other mutations may be present but they have no functional consequences on standard assays for Tcf4 activity.
Proper citation: RRID:Addgene_16514 Copy
Species: Homo sapiens
Genetic Insert: TCF4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:0; Vector Backbone:pcDNA?; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The TCF4 in this construct has a Q27R mutation. It is not known to affect the function.
Proper citation: RRID:Addgene_16512 Copy
Species: Homo sapiens
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: SW48 has a C-to-A missense mutation that changes Ser33 to Tyr.
Note that the NheI site is not intact and cannot be used for sub-cloning.
Proper citation: RRID:Addgene_16519 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: COV E
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165123 Copy
Species: SARS-CoV2
Genetic Insert: Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
Vector Backbone Description: Vector Backbone:pET-30a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_165088 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: ORF3a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165121 Copy
Species: SARS-CoV2
Genetic Insert: Severe acute respiratory syndrome coronavirus 2 ORF8
Vector Backbone Description: Vector Backbone:pET-30a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_165089 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: ORF9b
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165122 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: Nsp13 deltaN1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165127 Copy
Species: Synthetic
Genetic Insert: CT61 gene circuit
Vector Backbone Description: Backbone Size:2750; Vector Backbone:pSB3K3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please note that the GFP (D117G) and LuxR (S116A, V135I, M174I) sequence variants do not affect plasmid function.
Proper citation: RRID:Addgene_165403 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: Nsp4 deltaC
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165126 Copy
Species:
Genetic Insert: Nuclear Localization Signal
Vector Backbone Description: Backbone Size:4105; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Avian, Xenopus, Zebrafish; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165401 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: Nsp15
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165120 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:6594; Vector Types:Worm Expression, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD129.27, Ligation number L4437.
Proper citation: RRID:Addgene_1652 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2790; Vector Types:Worm Expression, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD129.36, Ligation number L4440. "Author's Map" courtesy of Dr. Guy Caldwell.
Proper citation: RRID:Addgene_1654 Copy
Species: Synthetic
Genetic Insert: CT67 gene circuit
Vector Backbone Description: Backbone Size:2750; Vector Backbone:pSB3K3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please note that the GFP (D117G) sequence variant does not affect plasmid function.
Proper citation: RRID:Addgene_165406 Copy
Species: Synthetic
Genetic Insert: CT82 gene circuit
Vector Backbone Description: Backbone Size:2750; Vector Backbone:pSB3K3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please note that the GFP (D117G) sequence variant does not affect plasmid function.
Proper citation: RRID:Addgene_165407 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: Orf9b L52D
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165129 Copy
Species: Synthetic
Genetic Insert: IC15 gene circuit
Vector Backbone Description: Backbone Size:2750; Vector Backbone:pSB3K3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please note that the GFP (D117G) and LuxR (S116A, V135I, M174I) sequence variants do not affect plasmid function.
Proper citation: RRID:Addgene_165408 Copy
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