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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pCDF-mTET1
 
Resource Report
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1+ mentions
RRID:Addgene_81052 TET1 Mus musculus Streptomycin PMID:26932196 Backbone Marker:Novagen; Backbone Size:3780; Vector Backbone:pCDF-Duet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-01 01:19:03 1
pCDF-casBCDE(+6)
 
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RRID:Addgene_89728 CRISPR array Other Streptomycin PMID:27738137 Backbone Marker:PMID:26013814; Vector Backbone:pCDF-casBCDE; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin Derivative CRISPR array containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end. 2026-08-01 01:20:28 0
pAF-HdrB3
 
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RRID:Addgene_184908 Cas9-sgRNA-homology arms Synthetic Streptomycin PMID:37556825 Please visit https://www.biorxiv.org/content/10.1101/2022.03.14.484339v1 for bioRxiv preprint. Vector Backbone:pJRD215; Vector Types:Bacterial Expression, CRISPR, broad-host-range; Bacterial Resistance:Streptomycin 2026-08-01 01:22:10 0
pREDawn-StrR-MCS
 
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RRID:Addgene_188979 Streptomycin PMID:35998606 Please note that Addgene's NGS found a 65bp insertion in the ori region as compared to the Depositor's sequence. The depositor has confirmed that the plasmid functions as described in the associated publication. Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-01 01:22:23 0
NCH1exp
 
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RRID:Addgene_131017 NCH1 Zea mays (maize) Streptomycin PMID:33679862 Backbone Size:11546; Vector Backbone:pH7WG2; Vector Types:Plant Expression; Bacterial Resistance:Streptomycin 2026-08-01 01:21:18 0
pCDF-Leup
 
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RRID:Addgene_213154 Leupeptin Protease Inhibitor Pathway Xenorhabdus bovienii SS-2004 Streptomycin PMID:34581573 Backbone Marker:Novagen (EMD Millipore); Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-01 01:28:11 0
pRoRH
 
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RRID:Addgene_213133 Streptomycin PMID:39126322 Backbone Size:5267; Vector Backbone:CloDF13; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Streptomycin 2026-08-01 01:28:37 0
pAurora2
 
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RRID:Addgene_213134 Streptomycin PMID:39126322 Backbone Size:6291; Vector Backbone:CloDF13; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Streptomycin 2026-08-01 01:28:37 0
pS44i8GH-2
 
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RRID:Addgene_207526 oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP Streptomycin PMID:38180826 Backbone Marker:SEVA collection; Vector Backbone:pSEVA448; Vector Types:CRISPR, Pseudomonas vector; Bacterial Resistance:Streptomycin 2026-08-01 01:27:02 0
pS44i8GM
 
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RRID:Addgene_207527 oriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP Streptomycin PMID:38180826 Backbone Marker:SEVA collection; Vector Backbone:pSEVA448; Vector Types:CRISPR, Pseudomonas vector; Bacterial Resistance:Streptomycin 2026-08-01 01:27:02 0
pEditA-RY
 
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RRID:Addgene_207533 xylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA Streptomycin PMID:38180826 Backbone Marker:SEVA collection; Vector Backbone:pSEVA448; Vector Types:CRISPR, Pseudomonas vector; Bacterial Resistance:Streptomycin 2026-08-01 01:27:02 0
pEditC-RY
 
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RRID:Addgene_207534 xylS (cured of BsaI-sites), Pm→CBE:SpRY, PEM7→non-specific sgRNA Streptomycin PMID:38180826 Backbone Marker:SEVA collection; Vector Backbone:pSEVA448; Vector Types:CRISPR, Pseudomonas vector; Bacterial Resistance:Streptomycin 2026-08-01 01:27:02 0
pEditC
 
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RRID:Addgene_207532 xylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA Streptomycin PMID:38180826 Backbone Marker:SEVA collection; Vector Backbone:pSEVA448; Vector Types:CRISPR, Pseudomonas vector; Bacterial Resistance:Streptomycin 2026-08-01 01:27:02 0
pSEVA43-GG
 
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RRID:Addgene_208014 msfGFP Streptomycin PMID:37975669 Please visit https://doi.org/10.1101/2023.06.29.547033 for bioRxiv preprint. Backbone Marker:SEVA collection; Backbone Size:3182; Vector Backbone:pSEVA231; Vector Types:; Bacterial Resistance:Streptomycin 2026-08-01 01:27:02 0
pCasso
 
Resource Report
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1+ mentions
RRID:Addgene_207530 cytidine base editing; oriV(pRO1600/ColE1), xylS, Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sgRNA Streptomycin PMID:38180826 Backbone Marker:SEVA collection; Vector Backbone:pSEVA448; Vector Types:CRISPR, Pseudomonas vector; Bacterial Resistance:Streptomycin 2026-08-01 01:27:31 1
pEPKKΩ1SP0678
 
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RRID:Addgene_187605 35s:TMVΩ:CUP2:GAl4:nos Synthetic Streptomycin PMID:37032497 + CBSmin35s:AAtrΔ11:35s + CBSmin35s: ATF1-2:mas + CBSmin35s:HarFar:g7 Please visit https://www.biorxiv.org/content/10.1101/2022.06.15.496242 for bioRxiv preprint. Vector Backbone:pDGB3_omega1; Vector Types:Plant Expression; Bacterial Resistance:Streptomycin 2026-08-01 01:27:34 0
HB101 endA::frt
 
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RRID:Addgene_45473 endA::frt Streptomycin PMID:21306445 endA was deleted for cleaner and higher yield plasmid preps. Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin major endonuclease endA deleted 2026-08-01 01:13:29 0
pTDpelB-CTwinStrep
 
Resource Report
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RRID:Addgene_45941 Streptomycin PMID:23687945 This plasmid was tested in the Gram-negative soil bacterium Pseudomonas putida KT2440 and Escherichia coli K12 and is especially suited for protein production, affinity purification, protein complex copurification with SPINE (Strep Protein Interaction Experiments) or (co-)localization studies. Due to the broad host range of the RK2 origin of replication, the plasmid facilitates experimental verification of hypothetical proteins and protein production yield assessment in different expression hosts possibly including new isolates. The Supplementary Table S1 in the following publication lists approximately 30 strains in which the RK2 origin of replication should be functional. Silva-Rocha et al., The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes. Nucleic Acids Research 2013, 41:D666-675. http://nar.oxfordjournals.org/content/41/D1/D666.long Backbone Marker:SEVA (de Lorenzo Lab); Vector Backbone:pSEVA424; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-01 01:13:40 0
pTD-C_sfYFPTwinStrep
 
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RRID:Addgene_45943 synthetic sfYFP (codon usage adapted to P.putida KT2440) Aequorea victoria Streptomycin PMID:23687945 This plasmid was tested in the Gram-negative soil bacterium Pseudomonas putida KT2440 and Escherichia coli K12 and is especially suited for protein production, affinity purification, protein complex copurification with SPINE (Strep Protein Interaction Experiments) or (co-)localization studies. Due to the broad host range of the RK2 origin of replication, the plasmid facilitates experimental verification of hypothetical proteins and protein production yield assessment in different expression hosts possibly including new isolates. The Supplementary Table S1 in the following publication lists approximately 30 strains in which the RK2 origin of replication should be functional. Silva-Rocha et al., The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes. Nucleic Acids Research 2013, 41:D666-675. http://nar.oxfordjournals.org/content/41/D1/D666.long Backbone Marker:Dammeyer et al. 2013; Vector Backbone:pTD-CTwinStrep; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-01 01:13:32 0
pTD-C_eYFPTwinStrep
 
Resource Report
Resource Website
RRID:Addgene_45942 synthetic eYFP (codon usage adapted to E.coli K12) Aequorea victoria Streptomycin PMID:23687945 This plasmid was tested in the Gram-negative soil bacterium Pseudomonas putida KT2440 and Escherichia coli K12 and is especially suited for protein production, affinity purification, protein complex copurification with SPINE (Strep Protein Interaction Experiments) or (co-)localization studies. Due to the broad host range of the RK2 origin of replication, the plasmid facilitates experimental verification of hypothetical proteins and protein production yield assessment in different expression hosts possibly including new isolates. The Supplementary Table S1 in the following publication lists approximately 30 strains in which the RK2 origin of replication should be functional. Silva-Rocha et al., The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes. Nucleic Acids Research 2013, 41:D666-675. http://nar.oxfordjournals.org/content/41/D1/D666.long Backbone Marker:Dammeyer et al. 2013; Vector Backbone:pTD-CTwinStrep; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-01 01:13:32 0

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